Application of heat-induced antigen retrieval on whole mount immuno-staining for adult mouse cerebellum

Current Neurobiology

Volumn 1, Issue 1, 2010, Pages 21-24

  Sawada, Kazuhiko ^a   Sun, Xue Zhi ^b  

^a TSUKUBA INTERNATIONAL UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

Author keywords

Antigen retrieval; Brain; Cerebellum; Immunohistochemistry; Whole mount

Indexed keywords

ANTIGEN; HEAT SHOCK PROTEIN 25; UNCLASSIFIED DRUG; ZEBRIN II;

ANIMAL TISSUE; ANTIGEN RETRIEVAL; ARTICLE; CEREBELLUM; CONTROLLED STUDY; DENDRITE; FREEZE THAWING; HEAT TREATMENT; IMMUNOHISTOCHEMISTRY; MALE; MOUSE; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; PURKINJE CELL;

EID: 77953618006     PISSN: 09759042     EISSN: 09761705     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Dent JA, Paulson AG, Klymkowsky MW. Whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus. Development 1989; 105: 61-74.
2. Davis CA, Holmyard DP, Millen KJ, Joyner AL. Examining pattern formation in mouse, chicken and frog embryos with an En-specific antiserum. Development 1991; 111: 287-298.
3. Luqué JM, Adams WB, Nicholls JG. Procedures for whole mount immunohistochemistry and in situ hybridization of immature mammalian CNS. Brain Res Brain Res Protocols 1998; 2: 165-173.
4. Piekut DT, Casey SM. Penetration of immunoreagents in Vibratome-sectioned brain: a light and electron microscopic study. J Histochem Cytochem. 1983; 31: 669-674.
5. Sillitoe RV, Hawkes R. Whole-mount immunohistocheistry: a high-throughput screen for patterning defects in the mouse cerebellum. J Histochem Cytochem 2002; 50: 235-244.
6. Sawada K, Fukui Y, Hawkes R. Spatial distribution of corticotropin-releasing factor immunopositive climbing fibers in the mouse cerebellum: analysis by whole mount immunohistochemistry. Brain Res 2008; 1222: 106-117.
7. Shi SR, Cote RJ, Taylor CR. Antigen retrieval techniques Current perspectives. J Histochem Cytochem 2001; 49: 931-937.
8. Brochu G, Maler L, Hawkes R. Zebrin II: a polypeptide antigen expressed selectively by Purkinje cells reveals compartments in rats and fish cerebellum. J Comp Neurol 1990; 291: 538-552.
9. Ahn AH, Dziennis S, Hawkes R, Herrup K. The cloning of zebrin II reveals its identity with aldolase C. Development 1994; 120: 2081-2090.
10. Hawkes R, Herrup K. Aldolase C/zebrin II and the regionalization of the cerebellum. J Mol Neurobiol 1996; 6: 147-158.
11. Armstrong CL, Krueger-Naug AM, Currie RW, Hawkes R. Constitutive expression of the 25-kDa heat shock protein Hsp25 reveals novel parasagittal bands of Purkinje cells in the adult mouse cerebellar cortex. J Comp Neurol 2000; 416: 383-397.
12. Sarna JR, Marzban H, Watanabe M, Hawkes R. Complementary stripes of phospholipase Cß3 and Cß4 expression by Purkinje cell subsets in the mouse cerebellum. J Comp Neurol 2006; 496: 303-313.