Development of a specific TaqMan® real-time PCR assay for quantification of Fusarium graminearum clade 7 and comparison of fungal biomass determined by PCR with deoxynivalenol content in wheat and barley

International Journal of Food Microbiology

Volumn 141, Issue 1-2, 2010, Pages 45-50

  Demeke, Tigst ^a   Gräfenhan, Tom ^b   Clear, Randy M ^a   Phan, Anh ^a   Ratnayaka, Indira ^a   Chapados, Julie ^b   Patrick, Susan K ^a   Gaba, Don ^a   Lévesque, C André ^b   Seifert, Keith A ^b  

^a CANADIAN GRAIN COMMISSION   (Canada)

^b OTTAWA RESEARCH AND DEVELOPMENT CENTRE   (Canada)

Author keywords

DON; Fusarium damaged kernel; Picogram DNA; Real time PCR

Indexed keywords

FUNGAL DNA; FUNGAL PROTEIN; MATING TYPE PROTEIN; UNCLASSIFIED DRUG; VOMITOXIN; TRICHOTHECENE DERIVATIVE;

ARTICLE; BARLEY; CANADA; CLADISTICS; CONTROLLED STUDY; DNA SEQUENCE; FUNGAL BIOMASS; FUSARIUM GRAMINEARUM; FUSARIUM HEAD BLIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; WHEAT; BIOMASS; FUSARIUM; GENETIC POLYMORPHISM; GENETICS; GENOTYPE; METHODOLOGY; MICROBIOLOGY; PHYLOGENY; PLANT DISEASE; PLANT SEED; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; VALIDATION STUDY; ACCURACY; COMPARATIVE STUDY; GENE SEQUENCE; GENETIC VARIABILITY; PHYLOGENETIC TREE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SINGLE NUCLEOTIDE POLYMORPHISM;

BASE SEQUENCE; BIOMASS; DNA, FUNGAL; FUNGAL PROTEINS; FUSARIUM; GENOTYPE; HORDEUM; PHYLOGENY; PLANT DISEASES; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; REPRODUCIBILITY OF RESULTS; SEEDS; SEQUENCE ANALYSIS, DNA; TRICHOTHECENES; TRITICUM;

FUSARIUM; GIBBERELLA ZEAE; HORDEUM; TRITICUM AESTIVUM;

EID: 77953540067     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2010.04.020     Document Type: Article

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