The effect of sericin from various extraction methods on cell viability and collagen production

International Journal of Molecular Sciences

Volumn 11, Issue 5, 2010, Pages 2200-2211

  Aramwit, Pornanong ^a   Kanokpanont, Sorada ^a   Nakpheng, Titpawan ^b   Srichana, Teerapol ^b  

^a CHULALONGKORN UNIVERSITY   (Thailand)

^b PRINCE OF SONGKLA UNIVERSITY   (Thailand)

Author keywords

Cell viability; Collagen; Concentration; Extraction; Sericin

Indexed keywords

COLLAGEN; SERICIN; UREA;

ANIMAL CELL; ARTICLE; CELL VIABILITY; COLLAGEN SYNTHESIS; CONTROLLED STUDY; CYTOTOXICITY; DRUG ISOLATION; DRUG MECHANISM; FIBROBLAST; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PARTICLE SIZE; ZETA POTENTIAL; ANIMAL; BIOSYNTHESIS; CELL LINE; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; DOSE RESPONSE; DRUG EFFECTS; ISOLATION AND PURIFICATION; METABOLISM;

ANIMALS; CELL LINE; CELL SURVIVAL; COLLAGEN; DOSE-RESPONSE RELATIONSHIP, DRUG; FIBROBLASTS; MICE; SERICINS; UREA;

EID: 77953513736     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms11052200     Document Type: Article

References (20)

1. Aramwit, P.; Kanokpanont, S.; De-Eknamkul, W.; Kamei, K.; Srichana, T. The effect of sericin with variable amino-acid content from different silk strains on the production of collagen and nitric oxide. J. Biomater. Sci. Polym. Ed. 2009, 20, 1295-1306.
2. Aramwit, P.; Sangcakul, A. The effects of sericin cream on wound healing in rats. Biosci Biotechnol. Biochem. 2007, 71, 2473-2477.
3. Terada, S.; Nishimura, T.; Sasaki, M.; Yamada, H.; Miki, M. Sericin, a protein derived from silkworms, accelerates the proliferation of several mammalian cell lines including a hybridoma. Cytotechnology 2002, 40, 3-12.
4. Tsubouchi, K.; Igarashi, Y.; Takasu, Y.; Yamada, H. Sericin enhances attachment of cultured human skin fibroblasts. Biosci. Biotechnol. Biochem. 2005, 69, 403-405.
5. Terada, S.; Sasaki, M.; Yanagihara, K.; Yamada, H. Preparation of silk protein sericin as mitogenic factor for better mammalian cell culture. J. Biosci. Bioeng. 2005, 100, 667-671.
6. Takahashi, M.; Tsujimoto, K.; Yamada, H.; Takagi, H.; Nakamori, S. The silk protein, sericin, protects against cell death caused by acute serum deprivation in insect cell culture. Biotechnol. Lett. 2003, 25, 1805-1809.
7. Sasaki, M.; Kato, Y.; Yamada, H.; Terada, S. Development of a novel serum-free freezing medium for mammalian cells using the silk protein sericin. Biotechnol. Appl. Biochem. 2005, 42, 183-188.
8. Kurioka, A.; Kurioka, F.; Yamazaki, M. Characterization of sericin powder prepared from citric acid-degraded sericin polypeptides of the silkworm, Bombyx Mori. Biosci. Biotechnol. Biochem. 2004, 68, 774-780.
9. Ogawa, A.; Terada, S.; Kanayama, T.; Miki, M.; Morikawa, M.; Kimura, T.; Yamaguchi, A.; Sasaki, M.; Yamada, H. Improvement of islet culture with sericin. J. Biosci. Bioeng. 2004, 98, 217-219.
10. Li, Y.G.; Ji, D.F.; Lin, T.B.; Zhong, S.; Hu, G.Y.; Chen, S. Protective effect of sericin eptide against alcohol-induced gastric injury in mice. Chin. Med. J. (Engl.) 2008, 121, 2083-2087.
11. Mason, R. Fabrics for atopic dermatitis. J. Fam. Health Care 2008, 18, 63-65.
12. Koller, D.Y.; Halmerbauer, G.; Bock, A.; Engstler, G. Action of a silk fabric treated with EGIS in children with atopic dermatitis: A 3-month trial. Pediatr. Allergy Immunol. 2007, 18, 335-338.
13. Sprague, K.U. The Bombyx mori silk proteins: Characterization of large polypeptides. Biochemistry 1975, 14, 925-931.
14. Zurovec, M.; Yang, C.; Kodrik, D.; Sehnal, F. Identification of a novel type of silk protein and regulation of its expression. J. Biol. Chem. 1998, 273, 15423-15428.
15. Nirmala, X.; Kodrik, D.; Zurovec, M.; Sehnal, F. Insect silk contains both a Kunitz-type and aunique Kazal-type proteinase inhibitor. Eur. J. Biochem. 2001, 268, 2064-2073.
16. Zeta Potential of Colloids in Water and Waste Water, ASTM Standard D 4187-82; American Society for Testing and Materials: West Conshohocken, PA, USA, 1985.
17. Franz, M.G.; Kuhn, M.A.; Nguyen, K.; Wang, X.; Ko, F.; Wright, T.E.; Robson, M.C. Transforming growth factor beta(2) lowers the incidence of incisional hernias. J. Surg. Res. 2001, 97, 109-116.
18. Henry, G.; Garner, W.L. Inflammatory mediators in wound healing. Surg. Clin. North. Am. 2003, 83, 483-507.
19. Takasu, Y.; Yamada, H.; Tsubouchi, K. Isolation of three main sericin components from the cocoon of the silkworm, Bombyx mori. Biosci. Biotechnol. Biochem. 2002, 66, 2715-2718.
20. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63.