Single-step affinity purification for fungal proteomics

Eukaryotic Cell

Volumn 9, Issue 5, 2010, Pages 831-833

  Liu, Hui Lin ^a,b   Osmani, Aysha H ^a   Ukil, Leena ^a,c   Son, Sunghun ^a   Markossian, Sarine ^a   Shen, Kuo Fang ^a   Govindaraghavan, Meera ^a   Varadaraj, Archana ^a   Hashmi, Shahr B ^a   de Souza, Colin P ^a   Osmani, Stephen A ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

^c GENOME INSTITUTE OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; ASPERGILLUS NIDULANS; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEOMICS; SACCHAROMYCES CEREVISIAE;

ASPERGILLUS NIDULANS; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FUNGAL PROTEINS; PROTEOMICS; SACCHAROMYCES CEREVISIAE;

EMERICELLA NIDULANS;

EID: 77953484190     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00032-10     Document Type: Article

References (12)

1. Kalman, S. M., K. Linderstrom-Lang, M. Ottesen, and F. M. Richards. 1955. Degradation of ribonuclease by subtilisin. Biochim. Biophys. Acta 16:297-299.
2. Kim, J. S., and R. T. Raines. 1993. Ribonuclease S-peptide as a carrier in fusion proteins. Protein Sci. 2:348-356.
3. Lee, S. B., and J. W. Taylor. 1990. Isolation of DNA from fungal mycelia and single spores, p. 282-287. In D. H. G. Michael A. Innis, J. J. Sninsky, and T. J. White (ed.), PCR protocols: a guide to methods and applications. Academic Press, Inc., Harcourt Brace Jovanovich, New York, NY.
4. Liu, H.-L., C. P. C. De Souza, A. H. Osmani, and S. A. Osmani. 2009. The three fungal transmembrane nuclear pore complex proteins of Aspergillus nidulans are dispensable in the presence of an intact An-Nup84-120 complex. Mol. Biol. Cell 20:616-630.
5. Nayak, T., E. Szewczyk, C. E. Oakley, A. Osmani, L. Ukil, S. L. Murray, M. J. Hynes, S. A. Osmani, and B. R. Oakley. 2006. A versatile and efficient gene-targeting system for Aspergillus nidulans. Genetics 172:1557-1566.
6. Osherov, N., and G. S. May. 1998. Optimization of protein extraction from Aspergillus nidulans for gel electrophoresis. Fungal Genet. Newsl. 45:38-40. http://www.fgsc.net/fgn45/45osherov.html.
7. Osmani, A. H., J. Davies, H. L. Liu, A. Nile, and S. A. Osmani. 2006. Systematic deletion and mitotic localization of the nuclear pore complex proteins of Aspergillus nidulans. Mol. Biol. Cell 17:4946-4961.
8. Richards, F. M. 1955. On an active intermediate produced during the digestion of ribonuclease by subtilisin. C. R. Trav. Lab. Carlsberg Chim. 29:329-346.
9. Richards, F. M. 1955. Titration of amino groups released during the digestion of ribonuclease by subtilisin. C. R. Trav. Lab. Carlsberg Chim. 29:322-328.
10. Son, S., and S. A. Osmani. 2009. Analysis of all protein phosphatase genes in Aspergillus nidulans identifies a new mitotic regulator, Fcp1. Eukaryot. Cell 8:573-585.
11. Ukil, L., A. Varadaraj, M. Govindaraghavan, H. L. Liu, and S. A. Osmani. 2008. Copy number suppressors of the Aspergillus nidulans nimA1 mitotic kinase display distinctive and highly dynamic cell cycle-regulated locations. Eukaryot. Cell 7:2087-2099.
12. Yang, L., L. Ukil, A. Osmani, F. Nahm, J. Davies, C. P. De Souza, X. Dou, A. Perez-Balaguer, and S. A. Osmani. 2004. Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans. Eukaryot. Cell 3:1359-1362.