In vitro effects of PDGF isoforms (AA, BB, AB and CC) on migration and proliferation of SaOS-2 osteoblasts and on migration of human osteoblasts

International Journal of Biomedical Science

Volumn 5, Issue 4, 2009, Pages 380-389

  Colciago, Alessandra ^a   Celotti, Fabio ^a   Casati, Lavinia ^a   Giancola, Rinaldo ^b   Castano, Stefano M ^b   Antonini, Guido ^b   Sacchi, Maria Cristina ^c   Negri Cesi, Paola ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b SAN CARLO BORROMEO HOSPITAL   (Italy)

^c C Arrigo Children's Hospital   (Italy)

Author keywords

Migration; Osteoblasts; PDGF isoforms; PRP; SaOS 2 cells

Indexed keywords

RECOMBINANT PLATELET DERIVED GROWTH FACTOR; RECOMBINANT PLATELET DERIVED GROWTH FACTOR AA; RECOMBINANT PLATELET DERIVED GROWTH FACTOR AB; RECOMBINANT PLATELET DERIVED GROWTH FACTOR BB; RECOMBINANT PLATELET DERIVED GROWTH FACTOR CC; UNCLASSIFIED DRUG;

ARTICLE; CELL ACTIVATION; CELL ASSAY; CELL CULTURE; CELL MIGRATION; CELL PROLIFERATION; CELL STIMULATION; CHEMOTAXIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; HUMAN; HUMAN CELL; IN VITRO STUDY; MITOGENESIS; OSTEOBLAST; OSTEOSARCOMA; REAL TIME POLYMERASE CHAIN REACTION; THROMBOCYTE RICH PLASMA;

EID: 77953459998     PISSN: 15509702     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Celotti F, Colciago A, Negri-Cesi P, et al. Effect of platelet-rich plasma on migration and proliferation of SaOS-2 osteoblasts: role of plateletderived growth factor and transforming growth factor-beta. Wound Repair Regen. 2006; 14:195-202.
2. Weibrich G, Kleis WK, Hafner G, et al. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 2002; 30: 97-102.
3. Frechette JP, Martineau I, Gagnon G. Platelet-rich plasmas: growth factor content and roles in wound healing. J Dent Res 2005; 84: 434-439.
4. Lacoste E, Martineau I, Gagnon G. Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release. J Periodontol 2003; 74: 1498-1507.
5. Fang L, Yan Y, Komuves LG, et al. PDGF C is a selective alpha platelet-derived growth factor receptor agonist that is highly expressed in platelet alpha granules and vascular smooth muscle. Arterioscler Thromb Vasc Biol 2004; 24: 787-792.
6. Li X, Eriksson U. Novel PDGF family members: PDGF-C and PDGF-D. Cytokine Growth Factor Rev 2003; 14: 91-98.
7. Borzini P, Mazzucco L. Platelet gels and releasates. Curr Opin Hematol 2005; 12: 473-79.
8. Yu Y, Harris RI, Yang JL, Anderson HC, Walsh WR. Differential expression of osteogenic factors associated with osteoinductivity of human osteosarcoma cell lines. J Biomed Mater Res A 2004; 70: 122-128.
9. McCarthy HS, Williams JH, Davie MW, Marshall MJ. Platelet-derived growth factor stimulates osteoprotegerin production in osteoblastic cells.. J Cell Physiol. 2009; 218(2):350-354.
10. Stabellini G, Vertemati M, Locci P, et al. In vitro human osteoblast and extracellular matrix changes after transforming growth factor beta 1 treatment. Pathology 2005; 37: 347-354.
11. Weibrich G, Kleis WK, Hitzler WE, Hafner G. Comparison of the platelet concentrate collection system with the plasma-rich-in-growth-factors kit to produce platelet-rich plasma: a technical report. Int J Oral Maxillofac Implants 2005; 20: 118-123.
12. Tonelli P, Mannelli D, Brancato L, Cinotti S, Morfini M. Counting of platelet derived growth factor and transforming growth factor-beta in platelet-rich-plasma used in jaw bone regeneration. Minerva Stomatol 2005; 54: 23-34.
13. Dugrillon A, Eichler H, Kern S, Kluter H. Autologous concentrated platelet-rich plasma (cPRP) for local application in bone regeneration. Int J Oral Maxillofac Surg 2002; 31: 615-619.
14. Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg 2004; 114: 1502-1508.
15. Fiedler J, Etzel N, Brenner RE. To go or not to go: Migration of human mesenchymal progenitor cells stimulated by isoforms of PDGF. J Cell Biochem 2004; 93: 990-8.
16. Caceres M, Hidalgo R, Sanz A, Martinez J, et al. Effect of Platelet-Rich Plasma on Cell Adhesion, Cell Migration, and Myofibroblastic Differentiation in Human Gingival Fibroblasts. J Periodontol 2008; 79: 714-720.
17. Goodkin DA, Pierce GF. Role of platelet derived growth factor in osteoblast function and bone synthesis: conflicting evidence. Wound Repair Regen 1993; 1: 203-212.
18. Uramoto H, Hackzell A, Wetterskog D, et al. pRb, Myc and p53 are critically involved in SV40 large T antigen repression of PDGF betareceptor transcription. J Cell Sci 2004; 117: 3855-3865.
19. Schmid C, Ghirlanda-Keller C, Zapf J. Effects of IGF-I and -II, IGF binding protein-3 (IGFBP-3), and transforming growth factor-beta (TGF-beta) on growth and apoptosis of human osteosarcoma Saos-2/ B-10 cells: lack of IGF-independent IGFBP-3 effects. Eur J Endocrinol 2001; 145: 213-221.
20. Ogino Y, Ayukawa Y, Kukita T, Koyano K. The contribution of platelet-derived growth factor, transforming growth factor-beta1, and insulin-like growth factor-I in platelet-rich plasma to the proliferation of osteoblast-like cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101: 724-729.