Development of cell-based assays for cytokine receptor signaling, using an AlphaScreen SureFire assay format

Analytical Biochemistry

Volumn 403, Issue 1-2, 2010, Pages 94-101

  Osmond, Ronald Ian William ^a   Das, Subhobrata ^a   Crouch, Michael Francis ^a  

^a TGR BioSciences   (Australia)

Author keywords

Cytokines; Homogeneous assay; Phosphorylation; STAT signaling

Indexed keywords

BIOACTIVITY; BLOOD; CELL CULTURE; CELLS; PHOSPHORYLATION; PROTEINS; SIGNALING;

CELLULAR ENVIRONMENT; CYTOKINES; ENDOGENOUS RECEPTORS; GROWTH FACTOR RECEPTORS; HOMOGENEOUS ASSAYS; RECEPTOR-MEDIATED SIGNALING; RESEARCH APPLICATIONS; SIGNAL TRANSDUCERS AND ACTIVATORS OF TRANSCRIPTIONS;

CELL SIGNALING;

ALPHA INTERFERON RECEPTOR; CYTOKINE RECEPTOR; GAMMA INTERFERON RECEPTOR; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR RECEPTOR; INTERLEUKIN 6 RECEPTOR; JANUS KINASE; STAT PROTEIN; STAT1 PROTEIN; STAT3 PROTEIN; STAT5 PROTEIN;

ACUTE MONOCYTIC LEUKEMIA; ARTICLE; CELL ASSAY; CONTROLLED STUDY; DRUG SCREENING; ERYTHROLEUKEMIA CELL; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; MEDICAL RESEARCH; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; WESTERN BLOTTING; BIOCHEMISTRY; JURKAT CELL LINE; METABOLISM; PHOSPHORYLATION; PROCEDURES; TUMOR CELL LINE;

BIOCHEMISTRY; BLOTTING, WESTERN; CELL LINE, TUMOR; HUMANS; JURKAT CELLS; PHOSPHORYLATION; RECEPTORS, CYTOKINE; SIGNAL TRANSDUCTION; STAT TRANSCRIPTION FACTORS; STAT1 TRANSCRIPTION FACTOR; STAT3 TRANSCRIPTION FACTOR; STAT5 TRANSCRIPTION FACTOR;

EID: 77953356208     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.04.007     Document Type: Article

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