Designing laboratory exercises for the undergraduate molecular biology/biochemistry student: Techniques and ethical implications involved in personalized medicine

Biochemistry and Molecular Biology Education

Volumn 38, Issue 3, 2010, Pages 180-187

  Weinlander, Kenneth M ^a   Hall, David J ^a  

^a LAWRENCE UNIVERSITY   (United States)

Author keywords

Ethics; Laboratory; Method; Per3; Personalized medicine

Indexed keywords

EID: 77953345284     PISSN: 14708175     EISSN: 15393429     Source Type: Journal    
DOI: 10.1002/bmb.20366     Document Type: Article

References (23)

1. P. Barry (2009) Seeking genetic fate, Sci. News 176, 16-21.
2. Personalized Medicine Coalition (PMC)-personalized medicine 101 (the overview). Available at:http://www.personalizedmedicinecoalition.org/sciencepolicy/personalmed-101_overview.php (revised 2009). Accessed June 30, 2009.
3. D. J. Lanska (2000) George Huntington (1850-1926) and hereditary chorea, J. Hist. Neurosci. 9, 76-89.
4. M. Frommer, L. E. McDonald, D. S. Millar, C. M. Collis, F. Watt, G. W. Grigg, P. L. Molloy, C. L. Paul (1992) A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands, Proc. Natl. Acad. Sci. USA 89, 1827-1831.
5. S. Herrero, J. L. Ivorra, M. Garcia-Sago, C. Martinez-Cortina (2008) Biochemistry and molecular biology techniques for person characterization, Biochem. Mol. Biol. Educ. 36, 347-353.
6. A. O. S. Lima, S. P. S Garcěs (2006) Intrageneric primer design: Bringing bioinformatics tools to the class, Biochem. Mol. Biol. Educ. 34, 332-337.
7. S. Imperial, B. Albert (2005) Determination of the Rh factor: A practical illustrating the use of the polymerase chan reaction, Biochem. Mol. Biol. Educ. 33, 50-53.
8. D. L. Frost, S. T. Pert (2003) DNA isolation from a dried blood ample, PCR amplification, and population analysis: Making the most of commercially available kits, Biochem. Mol. Biol. Educ. 31, 418-421.
9. PubMed Home. Available at: http://www.ncbi.nlm.nih.gov/pubmed/ (revised 2009). Accessed July 10, 2009.
10. GenBank Overview. Available at: http://www.ncbi.nlm.nih.gov/Gen bank/ (revised 2009). Accessed July 10, 2009.
11. HapMap Homepage. Available at: http://www.hapmap.org/ (revised 2009). Accessed July10,2009.
12. Online Mendelian Inheritance in Man. Available at: http://www.ncbi.nlm.nih.gov/omim. Accessed July 10, 2009.
13. Wolfram{pipe}Alpha. Available at: http://www.wolframalpha.com/ (revised 2009). Accessed July 10, 2009.
14. S. Arvidsson, M. Kwasniewski, D. M. Riano-Pachon, B. Mueller-Roeber (2008) QuantPrime-a flexible tool for reliable high-through-put primer design for quantitative PCR, BMC Bioinformatics 9, 465.
15. A. Untergasser, H. Nifveen, X. Rao, T. Bisseling, R. Geurts, J.A.M. Leunissen (2007) Primer3Plus, an enhanced web interface to Primer3, Nucleic Acids Res. 35, 71-74.
16. Primer Design Guide for PCR: Learn Designing Primers for PCR. Available at: http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html (revised 2009). Accessed July 10, 2009.
17. PCR Primer Design and PCR Setup. Available at: http://www.humgen.nl/primer_design.html (revised 2008). Accessed July 11, 2009.
18. Bio Basic Inc. DNA Sequencing Services. Available at: http:// www.biobasic.com/productIntro.jsp?pid=11 (revised 2009). Accessed July 11, 2009.
19. N. A. Nadkarni, M. E. Weale, M. von Schantz, M. G. Thomas (2005) Evolution of a length polymorphism in the human PER3 gene, a component of the circadian system, J. Biol. Rhythms 20, 490-499.
20. S. N. Archer, D. L. Robilliard, D. J. Skene, M. Smits, A. Williams, J. Arendt, M. G. von Schantz (2003) A length polymorphism in the circadian clock gene Per3 is linked to delayed sleep phase syndrome and extreme diurnal preference, Sleep 26, 413-415.
21. S. N. Archer, D. J. Dijk, J. Ellis, K. H. Jones, D. J. Skene, M. G. von Schantz (2007) Age-related change in the association between a polymorphism in the PER3 gene and preferred timing of sleep and waking activities, J. Sleep Res. 16, 12-16.
22. S. T. Chen, K. B. Choo, M. F. Hou, K. T. Yeh, S. J. Kuo, J. G. Chang (2005) Deregulated expression of the PER1, PER2 and PER3 genes in breast cancers, Carcinogenesis 26, 1241-1246.
23. Y. Zhu, H. N. Brown, Y. Zhang, R. G. Stevens, T. Zheng (2005) Period3 structural variation: A circadian biomarker associated with breast cancer in young women, Cancer Epidemiol. Biomarkers Prev. 14, 268-270.