Synaptonemal complex protein SYCP3 exists in two isoforms showing different conservation in mammalian evolution

Cytogenetic and Genome Research

Volumn 128, Issue 1-3, 2010, Pages 162-168

  Alsheimer, M ^a   Baier, A ^a,c   Schramm, S ^a   Schutz W ^b   Benavente, Ricardo ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c ZURICH UNIVERSITY OF APPLIED SCIENCES   (Switzerland)

Author keywords

Isoforms; Mammals; Meiosis; SYCP3; Synaptonemal complex

Indexed keywords

CELL PROTEIN; COMPLEMENTARY DNA; ISOPROTEIN; PROTEIN SYCP3; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; GENETIC CONSERVATION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN VARIANT; RAT; SEQUENCE ALIGNMENT; START CODON; SYNAPTONEMAL COMPLEX;

ANIMALS; BASE SEQUENCE; CERCOPITHECUS AETHIOPS; COS CELLS; EVOLUTION, MOLECULAR; MALE; MAMMALS; MICE; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PROTEIN ISOFORMS; RATS; SEQUENCE ALIGNMENT; SYNAPTONEMAL COMPLEX;

CRICETINAE; MAMMALIA; RATTUS; VERTEBRATA;

EID: 77953285894     PISSN: 14248581     EISSN: None     Source Type: Journal    
DOI: 10.1159/000303341     Document Type: Article

References (36)

1. Baier A, Alsheimer M, Volff JN, Benavente R: Synaptonemal complex protein SYCP3 of the rat: evolutionarily conserved domains and the assembly of higher order structures. Sex Dev 1: 161-168 (2007a).
2. Baier A, Alsheimer M, Benavente R: Synaptonemal complex protein SYCP3: conserved polymerization properties among vertebrates. Biochim Biophys Acta 1774: 595-602 (2007b).
3. Bolcun-Filas E, Costa Y, Speed R, Taggart M, Benavente R, et al.: SYCP2 is required for synaptonemal complex assembly, DSB repair and homologous recombination. J Cell Biol 176: 741-747 (2007).
4. Bolcun-Filas E, Hall E, Speed R, Taggart M, Grey C, et al.: Mutation of the mouse Syce1 gene disrupts synapsis and suggests a link between synaptonemal complex structural components and DNA repair. PLoS Genet 5:e1000393 (2009).
5. Botelho RJ, DiNicolo L, Tsao N, Karaiskakis A, Tarsounas M, et al.: The genomic structure of SYCP3 , a meiosis-specific gene encoding a protein of the chromosome core. Biochim Biophys Acta 1518: 294-299 (2001).
6. Costa Y, Cooke HJ: Dissecting the mammalian synaptonemal complex using targeted mutations. Chromosome Res 15: 579-589 (2007).
7. Costa Y, Speed R, Öllinger R, Alsheimer M, Semple CA, et al.: Two novel proteins recruited by synaptonemal complex protein 1 (SYCP1) are at the centre of meiosis. J Cell Sci 118: 2755-2762 (2005).
8. Cox J, Mann M: MaxQuant enables high peptide identification rates, individualized p.p.b.- range mass accuracies and proteome-wide protein quantification. Nat Biotechnol 26: 1367-1372 (2008).
9. Cox J, Matic I, Hilger M, Nagaraj N, Selbach M, et al.: A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics. Nat Protoc 4: 698-705 (2009).
10. de Vries FA, de Boer E, van den Bosch M, Baarends WM, Ooms M, et al.: Mouse Sycp1 functions in synaptonemal complex assembly, meiotic recombination, and XY body formation. Genes Dev 19: 1376-1389 (2005).
11. Dobson MJ, Pearlman RE, Karaiskakis A, Spyropoulos B, Moens PB: Synaptonemal complex proteins: occurrence, epitope mapping and chromosome disjunction. J Cell Sci 107: 2749-2760 (1994).
12. Hamer G, Gell K, Kouznetsova A, Novak I, Benavente R, Höög C: Characterization of a novel meiosis-specific protein within the central element of the synaptonemal complex. J Cell Sci 119: 4025-4032 (2006).
13. Hamer G, Wang H, Bolcun-Filas E, Cooke HJ, Benavente R, Höög C: Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex. J Cell Sci 121: 2445-2451 (2008).
14. Heyting C, Moens PB, van Raamsdonk W, Dietrich AJJ, Vink ACG, Redeker EJW: Identification of two major components of the lateral elements of synaptonemal complexes of the rat. Eur J Cell Biol 43: 148-154 (1987).
15. Ishihama Y, Rappsilber J, Andersen JS, Mann M: Microcolumns with self-assembled particle frits for proteomics. J Chromatogr A 979: 233-239 (2002).
16. Iwai T, Yoshii A, Yokota T, Sakai C, Hori H, et al.: Structural components of the synaptonemal complex, SYCP1 and SYCP3, in the medaka fish Oryzias latipes . Exp Cell Res 312: 2528-2537 (2006).
17. Kozak M: The scanning model for translation: an update. J Cell Biol 108: 229-241 (1989).
18. Lammers JH, Offenberg HH, van Aalderen M, Vink AC, Dietrich AJ, Heyting C: The gene encoding a major component of the lateral elements of synaptonemal complexes of the rat is related to X-linked lymphocyte-regulated genes. Mol Cell Biol 14: 1137-1146 (1994).
19. Lammers JH, van Aalderen M, Peters AH, van Pelt AA, Gaemers IC, et al.: A change in the phosphorylation pattern of the 30000-33000 Mr synaptonemal complex proteins of the rat between early and mid-pachytene. Chromosoma 104: 154-163 (1995).
20. Liebe B, Alsheimer M, Höög C, Benavente R, Scherthan H: Telomere attachment, meiotic chromosome condensation, pairing and bouquet stage duration are modified in spermatocytes lacking axial elements. Mol Biol Cell 15: 827-837 (2004).
21. Meuwissen RL, Offenberg HH, Dietrich AJ, Riesewijk A, van Iersel M, et al.: A coiled-coil related protein specific for synapsed regions of meiotic prophase chromosomes. EMBO J 11: 5091-5100 (1992).
22. Miyamoto T, Hasuike MP, Yogev L, Maduro MR, Ishikawa M, et al.: Azoospermia in patients heterozygous for a mutation in SYCP3 . Lancet 362: 1714-1719 (2003).
23. Offenberg HH, Schalk JA, Meuwissen RL, van Aalderen M, Kester HA, et al.: SCP2: a major protein component of the axial elements of synaptonemal complexes of the rat. Nucleic Acids Res 26: 2572-2579 (1998). (Pubitemid 28297367)
24. Öllinger R, Alsheimer M, Benavente R: Mammalian protein SCP1 forms synaptonemal complex- like structures in the absence of meiotic chromosomes. Mol Biol Cell 16: 212-217 (2005).
25. Olsen JV, de Godoy LM, Li G, Macek B, Mortensen P, et al.: Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap. Mol Cell Proteomics 4: 2010-2021 (2005).
26. Page SL, Hawley RS: The genetics and molecular biology of the synaptonemal complex. Annu Rev Cell Dev Biol 20: 525-558 (2004).
27. Perkins DN, Pappin DJ, Creasy DM, Cottrell JS: Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20: 3551-3567 (1999).
28. Rappsilber J, Ishihama Y, Mann M: Stop and go extraction tips for matrix-assisted laser desorption/ ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics. Anal Chem 75: 663-670 (2003).
29. Rappsilber J, Mann M, Ishihama Y: Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat Protoc 2: 1896- 1906 (2007).
30. Schalk JA, Dietrich AJ, Vink AC, Offenberg HH, van Aalderen M, Heyting C: Localization of SCP2 and SCP3 protein molecules within synaptonemal complexes of the rat. Chromosoma 107: 540-548 (1998).
31. Winkel K, Alsheimer M, Öllinger R, Benavente R: Protein SYCP2 provides a link between transverse filaments and lateral elements of mammalian synaptonemal complexes. Chromosoma 118: 259-267 (2009).
32. Yang F, De La Fuente R, Leu NA, Baumann C, McLaughlin KJ, Wong PJ: Mouse SYCP2 is required for synaptonemal complex assembly and chromosomal synapsis during male meiosis. J Cell Biol 173: 497-507 (2006).
33. Yuan L, Pelttari J, Brundell E, Björkroth B, Zhao J, et al.: The synaptonemal complex protein SCP3 can form multistranded, cross-striated fibers in vivo. J Cell Biol 142: 331-339 (1998).
34. Yuan L, Liu JG, Zhao J, Brundell E, Daneholt B, Höög C: The murine SCP3 gene is required for synaptonemal complex assembly, chromosome synapsis, and male fertility. Mol Cell 5: 73-83 (2000).
35. Zheng YH, Rengaraj D, Choi JW, Park KJ, Lee SI, Han JY: Expression pattern of meiosis associated SYCP family members during germ line development in chickens. Reproduction 138: 483-492 (2009).
36. Zickler D, Kleckner N: Meiotic chromosomes: integrating structure and function. Annu Rev Genet 33: 603-754 (1999).