Quantification of five putative periodontal pathogens in female patients with and without chronic periodontitis by real-time polymerase chain reaction

Anaerobe

Volumn 16, Issue 3, 2010, Pages 234-239

  Braga, Renato R R ^a   Carvalho, Maria Auxiliadora R ^a   Bruña Romero, Oscar ^a   Teixeira, Rodrigo E ^a   Costa, José Eustáquio ^a   Mendes, Edilberto N ^a   Farias, Luiz M ^a   Magalhães, Paula P ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

Chronic periodontitis; Oral microbiota; Periodontal diseases; Periodontal pathogens; Real time PCR

Indexed keywords

BACTERIAL DNA;

ADULT; AGGREGATIBACTER ACTINOMYCETEMCOMITANS; ARTICLE; BACTERIAL COUNT; BACTERIAL FLORA; CHRONIC PERIODONTITIS; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE ASSOCIATION; EIKENELLA CORRODENS; FEMALE; FUSOBACTERIUM NUCLEATUM; HUMAN; LOWEST INCOME GROUP; NONHUMAN; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MIDDLE AGED; PERIODONTAL POCKET; POLYMERASE CHAIN REACTION;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ADULT; CHRONIC PERIODONTITIS; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; EIKENELLA CORRODENS; FEMALE; FUSOBACTERIUM NUCLEATUM; HUMANS; MIDDLE AGED; PERIODONTAL POCKET; POLYMERASE CHAIN REACTION; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA; AGGREGATIBACTER ACTINOMYCETEMCOMITANS;

EIKENELLA CORRODENS; FUSOBACTERIUM NUCLEATUM; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA;

EID: 77953233154     PISSN: 10759964     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2010.02.007     Document Type: Article

References (29)

1. Albandar J.M., Rams T.E. Global epidemiology of periodontal diseases: an overview. Periodontology 2000, 2002(29):7-10.
2. Mombelli A., Casagni F., Madianos P.N. Can presence or absence of periodontal pathogens distinguish between subjects with chronic and aggressive periodontitis? A systematic review. J Clin Periodontol 2002, 29(Suppl. 3):10-21.
3. Nonnenmacher C., Dalpke A., Mutters R., Heeg K. Quantitative detection of periodontopathogens by real-time PCR. J Microbiol Methods 2004, 59:117-125.
4. Pihlstrom B.J., Michalowicz B.S., Johnson N.W. Periodontal diseases. Lancet 2005, 366:1809-1820.
5. Armitage G.C. Periodontal diagnoses and classification of periodontal diseases. Periodontology 2000, 2004(34):9-21.
6. Boutaga K., van Winkelhoff A.J., Vandenbroucke-Grauls C.M., Savelkoul P.H. The additional value of real-time PCR in the quantitative detection of periodontal pathogens. J Clin Periodontol 2006, 33:427-433.
7. Nonnenmacher C., Dalpke A., Rochon J., Flores-de-Jacoby L., Mutters R., Heeg K. Real-time polymerase chain reaction for detection and quantification of bacteria in periodontal patients. J Periodontol 2005, 76:1542-1549.
8. Darveau R.P., Tanner A., Page R.C. The microbial challenge in periodontitis. Periodontol 2000, 1997(14):12-32.
9. Haffajee A.D., Bogren A., Hasturk H., Feres M., Lopez N.J., Socransky S.S. Subgingival microbiota of chronic periodontitis subjects from different geographic locations. J Clin Periodontol 2004, 31:996-1002.
10. Joshi V.M., Vandana K.L. The detection of eight putative periodontal pathogens in adult and rapidly progressive periodontitis patients: an institutional study. Indian J Dent Res 2007, 18:6-10.
11. Fox J.G., Dewhirst F.E., Fraser G.J., Paster B.J., Shames B., Murphy J.C. Intracellular Campylobacter-like organism from ferrets and hamsters with proliferative bowel disease is a Desulfovibrio sp. J Clin Microbiol 1994, 32:1229-1237.
12. Kato H., Yoshida A., Awano S., Ansai T., Takehara T. Quantitative detection of volatile sulfur compound-producing microorganisms in oral specimens using real-time PCR. Oral Dis 2005, 11:67-71.
13. Kozarov E., Sweier D., Shelburne C., Progulske-Fox A., Lopatin D. Detection of bacterial DNA in atheromatous plaques by quantitative PCR. Microbes Infect 2006, 8:687-693.
14. Maeda H., Fujimoto C., Haruki Y., Maeda T., Kokeguchi S., Petelin M., et al. Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria. FEMS Immunol Med Microbiol 2003, 39:81-86.
15. Oral pathogen sequence databases http://www.oralgen.lanl.gov.
16. Aimetti M., Romano F., Nessi F. Microbiologic analysis of periodontal pockets and carotid atheromatous plaques in advanced chronic periodontitis patients. J Periodontol 2007, 78:1718-1723.
17. Doĝan B., Antinheimo J., Çetiner D., Bodur A., Emingil G., Buduneli E., et al. Subgingival microflora in Turkish patients with periodontitis. J Periodontol 2003, 74:803-814.
18. Ishihara K., Nabuchi A., Ito R., Miyachi K., Kuramitsu H.K., Okuda K. Correlation between detection rates of periodontopathic bacterial DNA in carotid coronary stenotic artery plaque and in dental plaque samples. J Clin Microbiol 2004, 42:1313-1315.
19. Lafaurie G.I., Contreras A., Barón A., Botero J., Mayorga-Fayad I., Jaramillo A., et al. Demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in Colombia: a multicenter study. J Periodontol 2007, 78:629-639.
20. Ledder R.G., Gilbert P., Huws S.A., Aarons L., Ashley M.P., Hull P.S., et al. Molecular analysis of the subgingival microbiota in health and disease. Appl Environ Microbiol 2007, 73:516-523.
21. Tanner A.C.R., Kent R., Kanasi E., Lu S.C., Paster B.J., Sonis S.T., et al. Clinical characteristics and microbiota of progressing slight chronic periodontitis in adults. J Clin Periodontol 2007, 34:917-930.
22. Ximenez-Fyvie L.A., Almaguer-Flores A., Jacobo-Soto V., Lara-Cordoba M., Sanchez-Vargas L.O., Alcantara-Maruri E. Description of the subgingival microbiota of periodontally untreated mexican subjects: chronic periodontitis and periodontal health. J Periodontol 2006, 77:460-471.
23. Cortelli J.R., Cortelli S.C., Jordan S., Haraszthy V.I., Zambon J.J. Prevalence of periodontal pathogens in Brazilians with aggressive or chronic periodontitis. J Clin Peridontol 2005, 32:860-866.
24. Cortelli J.R., Aquino D.R., Cortelli S.C., Carvalho-Filho J., Roman-Torres C.V.G., Costa F.O. A double-blind randomized clinical trial of subgingival minocycline for chronic periodontitis. J Oral Sci 2008, 50:259-265.
25. Vieira E.M., Raslan S.A., Wahasugui T.C., Avila-Campos M.J., Marvulle V. Gaetti-Jardim Júnior E. Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from Umutina Reservation, Mato Grosso, Brazil. J Appl Oral Sci 2009, 17:440-445.
26. Riep B., Edesi-Neuß L., Claessen F., Skarabis H., Ehmke B., Flemmig T.F., et al. Are putative periodontal pathogens reliable diagnostic markers?. J Clin Microbiol 2009, 47:1705-1711.
27. Colombo A.P.V., Teles R., Torres M.C., Souto R.M., Mendes M.C.S., Rosalem W., et al. Subgingival microbiota of Brazilian subjects with untreated chronic periodontitis. J Periodontol 2002, 73:360-369.
28. Kuboniwa M., Amano A., Kimura K.R., Sekine S., Kato S., Yamamoto Y., et al. Quantitative detection of periodontal pathogens using real-time polymerase chain reaction with TaqMan probes. Oral Microbiol Immunol 2004, 19:168-176.
29. Kawada M., Yoshida A., Suzuki N., Nakano Y., Saito T., Oho T., et al. Prevalence of Porphyromonas gingivalis in relation to periodontal status assessed by real-time PCR. Oral Microbiol Immunol 2004, 19:289-292.