Rapid multiple immunocytochemical staining method using microwave irradiation for intraoperative cytology

Acta Cytologica

Volumn 54, Issue 3, 2010, Pages 283-290

  Furuhata, Atsushi ^a   Sueyoshi, Noriyoshi ^a   Kurihara, Hidetake ^b   Sakamoto, Kazuhiro ^b   Kamano, Toshiki ^b   Hino, Okio ^b  

^a JUNTENDO UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b JUNTENDO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Adenocarcinoma; Immunocytochemistry; Intraoperative diagnosis; Microwaves; Washing cytology

Indexed keywords

ANTIBODY; BEREP4 ANTIBODY; CARCINOEMBRYONIC ANTIGEN; MOC 31 ANTIBODY; UNCLASSIFIED DRUG;

ADENOCARCINOMA; ARTICLE; CANCER PATIENT; CARCINOMA CELL; CLINICAL ASSESSMENT; COLORECTAL CANCER; CONCENTRATION (PARAMETERS); CYTOLOGY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; INTRAOPERATIVE PERIOD; MICROWAVE IRRADIATION; PERITONEUM; PRIORITY JOURNAL; RETROSPECTIVE STUDY; ROOM TEMPERATURE; SAMPLE; TIME;

EID: 77953184915     PISSN: 00015547     EISSN: 19382650     Source Type: Journal    
DOI: 10.1159/000325036     Document Type: Article

References (18)

1. Nomoto S, Nakao A, Takeuchi Y, Nonami T, Harada A, Ichihara T, Takagi H: Intraoperative peritoneal washing cytology with the rapid immunoperoxidase method using microwave irradiation. J Surg Oncol 1995;60:30-34
2. Selvaggi SM: Diagnostic pitfalls of peritoneal washing cytology and the role of cell blocks in their diagnosis. Diagn Cytopathol 2003;28:335-341
3. Nance KV, Silverman JF: Immunocytochemical panel for the identification of malignant cells in serous effusions. Am J Clin Pathol 1991;95:867-874
4. Muñoz TE, Giberson RT, Demaree R, Day JR: Microwave-assisted immunostaining: A new approach yields fast and consistent results. J Neurosci Methods 2004;137:133-139
5. Ruitenbeek T, Gouw ASH, Poppenma S: Immunocytology of body cavity fluids: MOC-31, a monoclonal antibody discriminating between mesotherial and epitherial cells. Arch Pathol Lab Med 1994;118:265-269 (Pubitemid 24093652)
6. Hecht JL, Pinkus JL, Pinkus GS: Monoclonal antibody MOC-31 reactivity as a marker for adenocarcinoma in cytologic preparations. Cancer Cytol 2006;108:56-59
7. Latza U, Niedobitek G, Schwarting R, Nakarda H, Stein H: Ber-EP4: New monoclonal antibody which distinguishes epithelia from mesothelia. J Clin Pathol 1990;43:213-219
8. Lozano MD, Panizo A, Tolodo GR, Sola JJ, Pardo-Mindán J: Immunocytochemistry in the differential diagnosis of serous effusions: A comparative evaluation of eight monoclonal antibodies in Papanicolaou stained smears. Cancer (Cancer Cytopathol) 2001;93:68-72
9. Broll R, Weschta M, Windhoevel U, Berndt S, Schwandner O, Roblick U, Schiedeck THK, Schimmelpenning H, Bruch HP, Duchrow M: Prognostic significance of free gastrointestinal tumor cells in peritoneal lavage detected by immunocytochemistry and polymerase chain reaction. Langenbeck's Arch Surg 2001;386:285-292
10. Van Der Loos CM, Becker AE, Van Den Oord JJ: Practical suggestions for successful immunoenzyme double-staining experiments. Histochem J 1993;25:1-13
11. Nakane PK: Simultaneous localization of multiple tissue antigens using the peroxidase labeled antibody methods: A study on pituitary gland of the rat. J Histochem Cytochem 1968;16:557-560
12. Wood GS, Warnke R: Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem 1981;29:1196-1204
13. Kumada T, Tsuneyama K, Hatta H, Ishizawa S, Takano Y: Improved 1-h rapid immunostaining method using intermittent microwave irradiation: Practicability based on 5 years application in Toyama Medical and Pharmaceutical University Hospital. Mod Pathol 2004;17:1141-1149
14. Boon ME, Kok LP: Microwaves for immunohistochemistry. Micron 1994;25:151-170
15. Lechago J, Sun NCJ, Weinstein WM: Simultaneous visualization of two antigens in the same tissue section by combining immunoperoxidase with immunofluorescence techniques. J Histochem Cytochem 1979;27:1221-1225 (Pubitemid 10224077)
16. Kobayashi K, Nakanishi H, Inada K, Fujimitsu Y, Yamachika T, Shirai T, Tatematsu M: Growth characteristics in the initial stage of micrometastasis formation by bacterial LacZ genetagged rat prostatic adenocarcinoma cells. Jpn J Cancer Res 1996;87:1227-1234
17. 125I-5-Iodo-2′-deoxyuridine. J Natl Cancer Inst 1970;45:773-782
18. Guller U, Zajac P, Schnider A, Bösch B, Vorburger S, Zuber M, Spagnoli GC, Oertli D, Maurer R, Metzger U, Harder F, Heberer M, Marti WR: Disseminated single tumor cells as detected by real-time quantitative polymerase chain reaction represent a prognostic factor in patients undergoing surgery for colorectal cancer. Ann Surg 2002;236:768-776