A simple approach for preparing real-time PCR positive reaction controls for rare or emerging viruses

Journal of Clinical Virology

Volumn 48, Issue 3, 2010, Pages 193-197

  Whiley, David M ^a,b   Faux, Cassandra E ^a,b   Bialasiewicz, Seweryn ^a,b   Gould, Allan R ^a   Lambert, Stephen B ^a,b   Nissen, Michael D ^a,b,c   Sloots, Theo P ^a,b,c  

^a Queensland Children's Medical Research Institute   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c Pathology Queensland Central   (Australia)

Author keywords

Control; Influenza; PCR; Positive; Real time; Synthetic

Indexed keywords

VIRUS DNA; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; EQUINE HERPES VIRUS; HUMAN METAPNEUMOVIRUS; INFLUENZA VIRUS A H1N1; INTERMETHOD COMPARISON; NONHUMAN; PARAINFLUENZA VIRUS 2; PRIORITY JOURNAL; QUALITY CONTROL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS IDENTIFICATION;

COMMUNICABLE DISEASES, EMERGING; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; METAPNEUMOVIRUS; PARAINFLUENZA VIRUS 2, HUMAN; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; VARICELLOVIRUS; VIROLOGY; VIRUS DISEASES; VIRUSES;

EID: 77953120104     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2010.03.022     Document Type: Article

References (5)

1. Smith G., Smith I., Harrower B., Warrilow D., and Bletchly C. A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents. J Virol Methods 135 (2006) 229-234
2. Whiley D.M., Bialasiewicz S., Bletchly C., Faux C.E., Harrower B., Gould A.R., et al. Detection of novel influenza A(H1N1) virus by real-time RT-PCR. J Clin Virol 45 (2009) 203-204
3. Maertzdorf J., Wang C.K., Brown J.B., Quinto J.D., Chu M., de Graaf M., et al. Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages. J Clin Microbiol 42 (2004) 981-986
4. Lambert S.B., Whiley D.M., O'Neill N.T., Andrews E.C., Canavan F.M., Bletchly C., et al. Comparing nose-throat swabs and nasopharyngeal aspirates collected from children with symptoms for respiratory virus identification using real-time polymerase chain reaction. Pediatrics 122 (2008) e615-e620
5. Bialasiewicz S., Whiley D.M., Buhrer-Skinner M., Bautista C., Barker K., Aitken S., et al. A novel gel-based method for self-collection and ambient temperature postal transport of urine for PCR detection of Chlamydia trachomatis. Sex Transm Infect 85 (2009) 102-105