Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically

Biotechnology and Bioengineering

Volumn 106, Issue 2, 2010, Pages 319-325

  Love, Kerry Routenberg ^a   Panagiotou, Vasiliki ^a   Jiang, Bo ^b   Stadheim, Terrance A ^b   Love, J Christopher ^a,c  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b GlycoFi Incorporated   (United States)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

Clonal selection; Heterologous protein secretion; Microengraving; Pichia pastoris; Single cell; Yeast

Indexed keywords

ANALYTICAL METHOD; BIOTHERAPEUTICS; CELL CYCLE; CLONAL SELECTION; HETEROLOGOUS PROTEIN SECRETION; HETEROLOGOUS PROTEINS; METHYLOTROPHIC YEASTS; P. PASTORIS; PICHIA PASTORIS; SINGLE CELLS; SINGLECELL ANALYSIS; STOCHASTIC MANNER;

PROTEINS; YEAST;

CLONE CELLS;

IMMUNOGLOBULIN FC FRAGMENT;

ARTICLE; CELL ASSAY; CELL CYCLE; FUNGAL CELL; NONHUMAN; PICHIA PASTORIS; PROTEIN POLYMORPHISM; PROTEIN SECRETION;

CELL CULTURE TECHNIQUES; COMPUTER SIMULATION; EQUIPMENT DESIGN; GENE EXPRESSION PROFILING; MODELS, BIOLOGICAL; PICHIA; PROTEIN ARRAY ANALYSIS; RECOMBINANT PROTEINS; STOCHASTIC PROCESSES; SYSTEMS INTEGRATION;

PICHIA PASTORIS;

EID: 77953060680     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22688     Document Type: Article

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