Broad-host-range plasmids for red fluorescent protein labeling of gram-negative bacteria for use in the zebrafish model system

Applied and Environmental Microbiology

Volumn 76, Issue 11, 2010, Pages 3467-3474

  Singer, John T ^a   Phennicie, Ryan T ^a   Sullivan, Matthew J ^a,b   Porter, Laura A ^a,c   Shaffer, Valerie J ^a,d   Kim, Carol H ^a  

^a UNIVERSITY OF MAINE   (United States)

^b Quincy College   (United States)

^c UNIVERSITY OF VIRGINIA   (United States)

^d JACKSON LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTATION; DANIO RERIO; DNA FRAGMENT; DNA SEQUENCE ANALYSIS; GRAM-NEGATIVE BACTERIA; GREEN FLUORESCENT PROTEIN; HOST CELLS; HUMAN PATHOGENS; IMMUNE CELLS; INNATE IMMUNE SYSTEMS; MODEL SYSTEM; P.AERUGINOSA; PIGMENT PRODUCTION; PSEUDOMONAS AERUGINOSA; REAL TIME INTERACTIONS; RED FLUORESCENT PROTEINS; TAC PROMOTER; ZEBRAFISH;

BACTERIA; BACTERIOLOGY; CLONING; DNA; ESCHERICHIA COLI; FLUORESCENCE; FRUITS; GENES; LABELING; PAINT; PATHOGENS; PROTEINS;

DNA SEQUENCES;

BACTERIAL DNA; PHOTOPROTEIN; RED FLUORESCENT PROTEIN;

BACTERIUM; CYPRINID; HOST RANGE; IMMUNE SYSTEM; MOLECULAR ANALYSIS; PHENOTYPE; PLASMID; REAL TIME;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; DISEASE MODEL; DNA SEQUENCE; GENE VECTOR; GENETICS; GRAM NEGATIVE BACTERIUM; HOST PATHOGEN INTERACTION; METABOLISM; METHODOLOGY; MICROBIOLOGY; MOLECULAR BIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PLASMID; STAINING; ZEBRA FISH; PROCEDURES;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; DISEASE MODELS, ANIMAL; DNA, BACTERIAL; GENETIC VECTORS; GRAM-NEGATIVE BACTERIA; HOST-PATHOGEN INTERACTIONS; LUMINESCENT PROTEINS; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; PLASMIDS; SEQUENCE ANALYSIS, DNA; STAINING AND LABELING; ZEBRAFISH;

BACTERIA (MICROORGANISMS); DANIO RERIO; ESCHERICHIA COLI; LISTONELLA ANGUILLARUM; LYCOPERSICON ESCULENTUM; NEGIBACTERIA; PSEUDOMONAS AERUGINOSA;

EID: 77953041738     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01679-09     Document Type: Article

References (31)

1. Bagdasarian, M. M., E. Amann, R. Lurz, B. Ruckert, and M. Bagdasarian. 1983. Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Gene 26:273-282.
2. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
3. Boyer, H. W., and D. Rolland-Dussoix. 1969. A complementation analysis of restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41: 459-472.
4. Clatworthy, A. E., J. S.-W. Lee, M. Leibman, Z. Kostun, A. J. Davidson, and D. T. Hung. 2009. Pseudomonas aeruginosa infection of zebrafish involves both host and pathogen determinants. Infect. Immun. 77:1293-1303.
5. Cohen, S. N., A. C. Y. Chang, and L. Hsu. 1972. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. USA 69:2110-2114.
6. Crosa,J.H.,L.L.Hodges,andM.H.Schiewe.1980. Curingofaplasmidiscorrelatedwithanattenuationofvirulenceinthemarinefishpathogen Vibrioanguillarum.Infect.Immun.27:897-902.(Pubitemid10147228)
7. Crosa, J. H., M. H. Schiewe, and S. Falkow. 1977. Evidence for plasmid contribution to the fish pathogen Vibrio anguillarum. Infect. Immun. 18:509-513.
8. De Boer, H. A., L. J. Comstock, and M. Vasser. 1983. The tac promoter: a functional hybrid derived from the trp and lac promoters. Proc. Natl. Acad. Sci. USA 80:21-25.
9. Ditta, G., S. Stanfield, D. Corbin, and D. R. Helinski. 1980. Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77:7347-7351. (Pubitemid 11189470)
10. Farabaugh, P. J. 1978. Sequence of the iacI gene. Nature 274:765-769.
11. Figurski, D. H., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76:1648-1652.
12. Fürste, J. P., W. Pansegrau, R. Frank, H. Blocker, P. Scholz, M. Bagdasarian, and E. Lanka. 1986. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48:119-131.
13. Herbomel,P.,B.Thisse,andC.Thisse.1999. Ontogenyandbehaviourofearlymacrophagesinthezebrafishembryo.Development126: 3735-3745.(Pubitemid29440473)
14. Karsi, A., S. Menanteua-Ledouble, and M. L. Lawrence. 2006. Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring. FEMS Microbiol. Lett. 260:216-223.
15. Lawson, N. D., and B. M. Weinstein. 2002. In vivo imaging of embryonic vascular development using transgenic zebrafish. Dev. Biol. 248:307-318.
16. Lin, B., S. Chen, Z. Cao, Y. Lin, D. Mo, H. Zhang, J. Gu, M. Dong, Z. Liu, and A. Xu. 2007. Acute phase response in zebrafish upon Aeromonas salmonicida and Staphylococcus aureus infection: striking similarities and obvious differences with mammals. Mol. Immunol. 44:295-301.
17. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
18. Neely, M. N., J. D. Pfeifer, and M. Caparon. 2002. Streptococcus- zebrafish model of bacterial pathogenesis. Infect. Immun. 70:3904-3914.
19. O'Farrell, P. H., E. Kutter, and M. Nakanishi. 1980. A restriction map of the bacteriophage T4 genome. Mol. Gen. Genet. 179:421-435.
20. O'Toole, R., J. Von Hofsten, R. Rosqvist, P. E. Olsson, and H. Wolf-Watz. 2004. Visualisation of zebrafish infection by GFP-labelled Vibrio anguillarum. Microb. Pathog. 37:41-46.
21. Pressley, M. E., P. E. Phelan III, P. E. Witten, M. T. Mellon, and C. H. Kim. 2005. Pathogenesis and inflammatory response to Edwardsiella tarda infection in the zebrafish. Dev. Comp. Immunol. 29:501-513.
22. Renshaw, S. A., C. A. Loynes, D. M. I. Trushell, S. Elworthy, P. W. Ingham, and M. K. B. Whyte. 2006. A transgenic zebrafish model of neutrophilic inflammation. Blood 108:3976-3978.
23. Russo, R., C. A. Shoemaker, V. S. Panagala, and P. H. Klesius. 2009. In vitro and in vivo interaction of macrophages from vaccinated and non-vaccinated channel catfish (Ictaluris punctatus) to Edwardsiella ictaluri. Fish Shellfish Immunol. 26:543-552.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Shaner, N. C., R. E. Campbell, P. A. Steinbach, B. N. G. Giepmans, A. E. Palmer, and R. Y. Tsien. 2004. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22:1567-1572.
26. Singer, J. T., W. Choe, K. A. Schmidt, and R. A. Makula. 1992. Virulence plasmid pJM1 prevents the conjugal entry of plasmid DNA into the marine fish pathogen Vibrio anguillarum 775. J. Gen. Microbiol. 138:2485-2490.
27. Singer, J. T., and S. Earley. 1989. Identification of polypeptides encoded by cloned pJM1 iron uptake DNA isolated from Vibrio anguillarum 775. J. Bacteriol. 171:2293-2302.
28. Singer, J. T., J. J. van Tuijl, and W. R. Finnerty. 1986. Transformation and mobilization of cloning vectors in Acinetobacter spp. J. Bacteriol. 165:301-303.
29. Southward, C. M., and M. G. Surette. 2002. The dynamic microbe: green fluorescent protein brings bacteria to light. Mol. Microbiol. 45:1191-1196.
30. van der Sar, A. M., R. J. Musters, F. J. van Eeden, B. J. Appelmelk, C. M. Vandenbroucke-Grauls, and W. Bitter. 2003. Zebrafish embryos as a model host for the real time analysis of Salmonella typhimurium infections. Cell. Microbiol. 5:601-611.
31. Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259-268. (Pubitemid 13201666)