Isolation and functional characterization of α-smooth muscle actin expressing cardiomyocytes from embryonic stem cells

Cellular Physiology and Biochemistry

Volumn 25, Issue 6, 2010, Pages 595-604

  Potta, Shiva Prasad ^a   Liang, Huamin ^a   Winkler, Johannes ^a   Doss, Michael Xavier ^a,b   Chen, Shuhua ^a   Wagh, Vilas ^a   Pfannkuche, Kurt ^a   Hescheler, Jürgen ^a   Sachinidis, Agapios ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

^b MASONIC MEDICAL RESEARCH LABORATORY   (United States)

Author keywords

Acta2; Cardiomyocytes; ES cells; Lineage selection; Patch clamp electrophysiology

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; ENHANCED GREEN FLUORESCENT PROTEIN; MYOSIN HEAVY CHAIN ALPHA; ACTIN; ACYLTRANSFERASE; GREEN FLUORESCENT PROTEIN; PUROMYCIN N-ACETYLTRANSFERASE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL FUNCTION; CELL ISOLATION; CELL POPULATION; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; FEMALE; HEART ATRIUM; HEART DEVELOPMENT; HEART MUSCLE CELL; HEART VENTRICLE; IN VITRO STUDY; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SMOOTH MUSCLE FIBER; ANIMAL; CELL LINE; CELL SEPARATION; CYTOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PATCH CLAMP TECHNIQUE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SMOOTH MUSCLE; TRANSGENE;

MAMMALIA;

ACETYLTRANSFERASES; ACTINS; ANIMALS; CELL LINE; CELL SEPARATION; EMBRYONIC STEM CELLS; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; MICE; MUSCLE, SMOOTH; MYOCYTES, CARDIAC; PATCH-CLAMP TECHNIQUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSGENES;

EID: 77952976590     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000315078     Document Type: Article

References (27)

1. Vandekerckhove J, K Weber: Mammalian cytoplasmic actins are the products of at least two genes and differ in primary structure in at least 25 identified positions from skeletal muscle actins. Proc Natl Acad Sci USA 1978;75:1106-1110.
2. Kashina AS: Differential arginylation of actin isoforms: the mystery of the actin N-terminus. Trends Cell Biol 2006;16:610-615.
3. Vandekerckhove J, K Weber: At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol 1978;126:783-802.
4. Chaponnier C, M Goethals, PA Janmey, F Gabbiani, G Gabbiani, J Vandekerckhove: The specific NH2-terminal sequence Ac-EEED of alpha-smooth muscle actin plays a role in polymerization in vitro and in vivo. J Cell Biol 1995;130:887-895.
5. Skalli O, P Ropraz, A Trzeciak, G Benzonana, D Gillessen, G Gabbiani: A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 1986;103:2787-2796.
6. Ruzicka DL, RJ Schwartz: Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation. J Cell Biol 1988;107:2575-2586.
7. Woodcock-Mitchell J, JJ Mitchell, RB Low, M Kieny, P Sengel, L Rubbia, O Skalli, B Jackson, G Gabbiani: Alpha-smooth muscle actin is transiently expressed in embryonic rat cardiac and skeletal muscles. Differentiation 1988;39:161-166.
8. Ya J, MW Markman, GT Wagenaar, PJ Blommaart, AF Moorman, WH Lamers: Expression of the smooth-muscle proteins alpha-smooth-muscle actin and calponin, and of the intermediate filament protein desmin are parameters of cardiomyocyte maturation in the prenatal rat heart. Anat Rec 1997;249:495-505.
9. Kumar A, K Crawford, L Close, M Madison, J Lorenz, T Doetschman, S Pawlowski, J Duffy, J Neumann, J Robbins, GP Boivin, BA O'Toole, JL Lessard: Rescue of cardiac alpha-actin-deficient mice by enteric smooth muscle gamma-actin. Proc Natl Acad Sci USA 1997;94:4406-4411.
10. Doss MX, J Winkler, S Chen, R Hippler-Altenburg, I Sotiriadou, M Halbach, K Pfannkuche, H Liang, H Schulz, O Hummel, N Hubner, R Rottscheidt, J Hescheler, A Sachinidis: Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes. Genome Biol 2007;8:R56.
11. Sachinidis A, BK Fleischmann, E Kolossov, M Wartenberg, H Sauer, J Hescheler: Cardiac specific differentiation of mouse embryonic stem cells. Cardiovasc Res 2003;58:278-291.
12. Potta SP, H Liang, K Pfannkuche, J Winkler, S Chen, MX Doss, K Obernier, N Kamisetti, H Schulz, N Hubner, J Hescheler, A Sachinidis: Functional characterization and transcriptome analysis of embryonic stem cell-derived contractile smooth muscle cells. Hypertension 2009;53:196-204.
13. Fleischmann M, W Bloch, E Kolossov, C Andressen, M Muller, G Brem, J Hescheler, K Addicks, BK Fleischmann: Cardiac specific expression of the green fluorescent protein during early murine embryonic development. FEBS Lett 1998;440:370-376.
14. Horn R, A Marty: Muscarinic activation of ionic currents measured by a new whole-cell recording method. J Gen Physiol 1988;92:145-159.
15. Clement S, M Stouffs, E Bettiol, S Kampf, KH Krause, C Chaponnier, M Jaconi: Expression and function of alpha-smooth muscle actin during embryonic-stem-cell-derived cardiomyocyte differentiation. J Cell Sci 2007;120:229-238.
16. Kattman SJ, ED Adler, GM Keller: Specification of multipotential cardiovascular progenitor cells during embryonic stem cell differentiation and embryonic development. Trends Cardiovasc Med 2007;17:240-246.
17. Colas JF, A Lawson, GC Schoenwolf: Evidence that translation of smooth muscle alpha-actin mRNA is delayed in the chick promyocardium until fusion of the bilateral heart-forming regions. Dev Dyn 2000;218:316-330.
18. Kalajzic Z, H Li, LP Wang, X Jiang, K Lamothe, DJ Adams, HL Aguila, DW Rowe, I Kalajzic: Use of an alpha-smooth muscle actin GFP reporter to identify an osteoprogenitor population. Bone 2008;43:501-510.
19. Eppenberger-Eberhardt M, I Flamme, V Kurer, HM Eppenberger: Reexpression of alpha-smooth muscle actin isoform in cultured adult rat cardiomyocytes. Dev Biol 1990;139:269-278.
20. Leslie KO, DJ Taatjes, J Schwarz, M vonTurkovich, RB Low: Cardiac myofibroblasts express alpha smooth muscle actin during right ventricular pressure overload in the rabbit. Am J Pathol 1991;139:207-216.
21. Adachi S, H Ito, M Tamamori, M Tanaka, F Marumo, M Hiroe: Skeletal and smooth muscle alpha-actin mRNA in endomyocardial biopsy samples of dilated cardiomyopathy patients. Life Sci 1998;63:1779-1791.
22. Schram G, M Pourrier, P Melnyk, S Nattel: Differential distribution of cardiac ion channel expression as a basis for regional specialization in electrical function. Circ Res 2002;90:939-950.
23. Tolsa JF, M Marino, AC Peyter, JL Beny: Role of membrane potential in endothelium-dependent relaxation of isolated mouse main pulmonary artery. J Cardiovasc Pharmacol 2006;47:501-507.
24. Hashitani H, AF Brading: Ionic basis for the regulation of spontaneous excitation in detrusor smooth muscle cells of the guinea-pig urinary bladder. Br J Pharmacol 2003;140:159-169.
25. Hotta A, Y Kito, H Suzuki: The effects of flufenamic acid on spontaneous activity of smooth muscle tissue isolated from the guinea-pig stomach antrum. J Smooth Muscle Res 2005;41:207-220.
26. Marks TN, SW Jones: Calcium currents in the A7r5 smooth muscle-derived cell line. An allosteric model for calcium channel activation and dihydropyridine agonist action. J Gen Physiol 1992;99:367-390.
27. Halbach M, K Pfannkuche, F Pillekamp, A Ziomka, T Hannes, M Reppel, J Hescheler, J Muller-Ehmsen: Electrophysiological maturation and integration of murine fetal cardiomyocytes after transplantation. Circ Res 2007;101:484-492.