Effects of high intensity exercise on isoelectric profiles and SDS-PAGE mobility of erythropoietin

International Journal of Sports Medicine

Volumn 31, Issue 6, 2010, Pages 367-371

  Voss, S ^a   Ludke A ^a   Romberg, S ^a   Schanzer E ^a   Flenker, U ^a   Demarees, M ^a   Achtzehn, S ^a   Mester, J ^a   Schanzer W ^a  

^a GERMAN SPORT UNIVERSITY COLOGNE   (Germany)

Author keywords

Doping; Electrophoresis; Electrophoretic mobility; EPO; High intensity exercise; IEF profiles

Indexed keywords

ERYTHROPOIETIN;

ADULT; ARTICLE; BLOOD; DOPING; EXERCISE; EXERCISE TEST; FEMALE; GERMANY; HUMAN; ISOELECTRIC FOCUSING; MALE; METHODOLOGY; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; URINE;

ADULT; DOPING IN SPORTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROPOIETIN; EXERCISE TEST; FEMALE; GERMANY; HUMANS; ISOELECTRIC FOCUSING; MALE; PHYSICAL EXERTION;

EID: 77952962122     PISSN: 01724622     EISSN: 14393964     Source Type: Journal    
DOI: 10.1055/s-0030-1249083     Document Type: Article

References (16)

1. Bajla I, Holländer I, Minichmayr M, Gmeiner G, Reichel Ch. GASepo-a software solution for quantitative analysis of digital images in Epo doping control. Comput Methods Programs Biomed 2005 80 246-270
2. Belalcazar V, Gutiérrez Gallego R, Llop E, Segura J, Pascual JA. Assessing the instability of the isoelectric focusing patterns of erythropoietin in urine. Electrophoresis 2006 27 4387-4395
3. Beullens M, Delanghe JR, Bollen M. False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise. Blood 2006 15 4711-4713
4. Catlin D, Green G, Sekera M, Scott P, Starcevic B. False-positive Epo test concerns unfounded. Blood 2006 1 1778
5. Delanghe JR, Bollen M, Beullens M. Testing for recombinant erythropoietin. Am J Hematol 2008 83 237-241
6. Harriss DJ, Atkinson G. International Journal of Sports Medicine Ethical Standards in Sport and Exercise Science Research. Int J Sports Med 2009 30 701-702
7. http://news.bbc.co.uk/1/hi/sci/tech/7517332.stm 21.01.2009
8. Kohler M, Ayotte C, Desharnais P, Flenker U, Lüdke S, Thevis M, Völker-Schänzer E, Schänzer W. Discrimination of recombinant and endogenous urinary erythropoietin by calculating relative mobility values from SDS gels. Int J Sports Med 2008 29 1-6
9. Lamon S, Martin L, Robinson N, Saugy M, de Ceaurritz J, Lasne F. Effects of exercise on the isoelectric patterns of erythropoietin. Clin J Sport Med 2009 19 311-315
10. Lasne F, de Ceaurriz J. Recombinant erythropoietin in urine. Nature 2000 8 635
11. Lasne F. No doubt about the validity of the urine test for detection of recombinant erythropoietin. Blood 2006 1 1778-1779
12. rd Cologne Workshop on Dope Analysis. Cologne Sportverlag Strauss 2005 297-304
13. Poortmans JR, Rampaer L, Wolfs JC. Renal protein excretion after exercise in man. Eur J Appl Physiol 1989 58 476-480
14. Reichel C. Identification of zinc-alpha-2-glycoprotein binding to clone AE7A5 antihuman EPO antibody by means of nano-HPLC and high-resolution high-mass accuracy ESI-MS/MS. J Mass Spectrom 2008 43 916-923
15. Reichel C, Kulovics R, Jordan V, Watzinger M, Geisendorfer T. SDS-PAGE of recombinant and endogenous erythropoietins: benefits and limitations of the method for application in doping control. Drug Test Analysis 2009 1 43-50
16. WADA Technical Document TD2009EPO. http://www.wada-ama.org/rtecontent/ document/TD2009EPO-Sept2009-EN.pdf