Indole-based assay to assess the effect of ethanol on Pseudomonas putida F1 dioxygenase activity

Biodegradation

Volumn 21, Issue 3, 2010, Pages 425-430

  da Silva, Márcio Luis Busi ^a   Alvarez, Pedro J J ^b  

^a Desembargador Vitor Lima   (Brazil)

^b Rice University   (United States)

Author keywords

Bioremediation; Ethanol; Indigo production; Metabolic flux dilution; Toluene dioxygenase

Indexed keywords

A-CARBON; AEROBIC BIODEGRADATION; CATABOLITE REPRESSION; DIOXYGENASES; ETHANOL CONCENTRATIONS; METABOLIC FLUX; METABOLIC FLUX DILUTION; NATURALLY OCCURRING; PRODUCTION RATES; PSEUDOMONAS PUTIDA F1; REFORMULATED GASOLINE; SIMPLE APPROACH; SPECTROPHOTOMETRIC ASSAYS; SUBSTRATE RANGE; TOLUENE DIOXYGENASE; TOLUENE MIXTURES;

AROMATIC HYDROCARBONS; BENZENE; BIODEGRADATION; BIOREMEDIATION; BIOTECHNOLOGY; ETHANOL; ETHYLBENZENE; GASOLINE; GROUNDWATER; GROUNDWATER POLLUTION; METABOLISM; MICROBIOLOGY; POLLUTION; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY; XYLENE;

TOLUENE;

ALCOHOL; INDIGO; INDOLE; INDOLE DERIVATIVE; OXYGENASE; TOLUENE; TOLUENE DIOXYGENASE;

BACTERIUM; BIOREMEDIATION; DYE; ENZYME ACTIVITY; ETHANOL; GROUNDWATER POLLUTION; MICROBIAL ACTIVITY; OXIC CONDITIONS; SPECTROPHOTOMETRY; TOLUENE;

AEROBIC METABOLISM; ARTICLE; BIOREMEDIATION; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ASSAY; ENZYMOLOGY; METABOLISM; PSEUDOMONAS PUTIDA; SPECTROPHOTOMETRY;

AEROBIOSIS; BIODEGRADATION, ENVIRONMENTAL; ENZYME ASSAYS; ETHANOL; INDOLES; OXYGENASES; PSEUDOMONAS PUTIDA; SPECTROPHOTOMETRY; TOLUENE;

PSEUDOMONAS PUTIDA;

EID: 77952957233     PISSN: 09239820     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10532-009-9312-9     Document Type: Article

References (30)

1. Alvarez PJJ, Vogel TM (1991) Substrate interactions of benzene, toluene, and para-xylene during microbial-degradation by pure cultures and mixed culture aquifer slurries. Appl Environ Microbiol 57(10): 2981-2985.
2. Button DK (1985) Kinetics of nutrient-limited transport and microbial-growth. Microbiol Rev 49(3): 270-297.
3. Corseuil HX, Hunt CS, Dos Santos RCF, Alvarez PJJ (1998) The influence of the gasoline oxygenate ethanol on aerobic and anaerobic BTX biodegradation. Water Res 32(7): 2065-2072.
4. Costura RK, Alvarez PJJ (2000) Expression and longevity of toluene dioxygenase in Pseudomonas putida F1 induced at different dissolved oxygen concentrations. Water Res 34(11): 3014-3018.
5. Dorn JG, Frye RJ, Maier RM (2003) Effect of temperature, pH, and initial cell number on luxCDABE and nah gene expression during naphthalene and salicylate catabolism in the bioreporter organism Pseudomonas putida RB1353. Appl Environ Microbiol 69(4): 2209-2216.
6. Duetz WA, Dejong C, Williams PA, Vanandel JG (1994a) Competition in chemostat culture between Pseudomonas strains that use different pathways for the degradation of toluene. Appl Environ Microbiol 60(8): 2858-2863.
7. Duetz WA, Marques S, Dejong C, Ramos JL, Vanandel JG (1994b) Inducibility of the Tol catabolic pathway in Pseudomonas putida (Pww0) growing on succinate in continuous-culture-evidence of carbon catabolite repression control. J Bacteriol 176(8): 2354-2361.
8. Egli T (1995) The ecological and physiological significance of the growth of heterotrophic microorganisms with mixtures of substrates. Adv Microb Ecol 14: 305-386.
9. Egli T, Käppeli O, Fiechter A (1982) Regulatory flexibility of methylotrophic yeasts in chemostat cultures: simultaneous assimilation of glucose and methanol at a fixed dilution rate. Arch Microbiol 131: 1-7.
10. Egli T, Lindley ND, Quayle JR (1983) Regulation of enzyme synthesis and variation of residual methanol concentration during carbon-limited growth of Kloeckera sp. 2201 on mixtures of methanol and glucose. J Gen Microbiol 129: 1269-1281.
11. Egli T, Lendenmann U, Snozzi M (1993) Kinetics of microbial growth with mixtures of carbon sources. Antonie van Leeuwenhoek 63: 289-298.
12. Ensley BD, Gibson DT (1983) Naphthalene dioxygenase-purification and properties of a terminal oxygenase component. J Bacteriol 155(2): 505-511.
13. Finette BA, Subramanian V, Gibson DT (1984) Isolation and characterization of Pseudomonas putida Ppf1 mutants defective in the toluene dioxygenase enzyme-system. J Bacteriol 160(3): 1003-1009.
14. Gibson DT, Parales RE (2000) Aromatic hydrocarbon dioxygenases in environmental biotechnology. Curr Opin Biotechnol 11(3): 236-243.
15. Heitzer A, Applegate B, Kehrmeyer S, Pinkart H, Webb OF, Phelps TJ, White DC, Sayler GS (1998) Physiological considerations of environmental applications of lux reporter fusions. J Microbiol Methods 33(1): 45-57.
16. Hunt CS, Cronkhite LA, Corseuil HX, Alvarez PJJ (1997) Effect of ethanol on anaerobic toluene degradation in aquifer microcosms. Abstr Pap Am Chem Soc 213: 243.
17. Lau PCK, Bergeron H, Labbe D, Wang Y, Brousseau R, Gibson DT (1994) Sequence and expression of the tod gih genes involved in the last 3 steps of toluene degradation by Pseudomonas putida F1. Gene 146(1): 7-13.
18. Lovanh N, Alvarez PJJ (2004) Effect of ethanol, acetate, and phenol on toluene degradation activity and tod-lux expression in Pseudomonas putida TOD102: an evaluation of the metabolic flux dilution model. Biotechnol Bioeng 86(7): 801-808.
19. Lovanh N, Hunt CS, Alvarez PJJ (2002) Effect of ethanol on BTEX biodegradation kinetics: aerobic continuous culture experiments. Water Res 36(15): 3739-3746.
20. Madigan MT, Martinko JM, Parker J (1997) Brock biology of microorganisms. Prentice Hall, United States.
21. Mermod N, Ramos JL, Lehrbach PR, Timmis KN (1986) Vector for regulated expression of cloned genes in a wide range of gram-negative bacteria. J Bacteriol 167(2): 447-454.
22. Murdock D, Ensley BD, Serdar C, Thalen M (1993) Construction of metabolic operons catalyzing the denovo biosynthesis of indigo in Escherichia coli. Biotechnology 11(3): 381-386.
23. Neilson JW, Pierce SA, Maier RM (1999) Factors influencing expression of luxCDABE and nah genes in Pseudomonas putida RB1353 (NAH7, pUTK9) in dynamic systems. Appl Environ Microbiol 65(8): 3473-3482.
24. O'Connor KE, Dobson ADW, Hartmans S (1997) Indigo formation by microorganisms expressing styrene monooxygenase activity. Appl Environ Microbiol 63(11): 4287-4291.
25. Robertson BR, Button DK (1987) Toluene induction and uptake kinetics and their inclusion in the specific affinity relationship for describing rates of hydrocarbon metabolism. Appl Environ Microbiol 53(9): 2193-2205.
26. Ruiz-Aguilar GML, Fernandez-Sanchez JM, Kane SR, Kim D, Alvarez PJJ (2002) Effect of ethanol and methyl-tert-butyl ether on monoaromatic hydrocarbon biodegradation: response variability for different aquifer materials under various electron-accepting conditions. Environ Toxicol Chem 21(12): 2631-2639.
27. Wackett LP, Kwart LD, Gibson DT (1988) Benzylic monooxygenation catalyzed by toluene dioxygenase from Pseudomonas putida. Biochemistry 27(4): 1360-1367.
28. Woo HJ, Sanseverino J, Cox CD, Robinson KG, Sayler GS (2000) The measurement of toluene dioxygenase activity in biofilm culture of Pseudomonas putida F1. J Microbiol Methods 40(2): 181-191.
29. Zylstra GJ, McCombie WR, Gibson DT, Finette BA (1988) Toluene degradation by Pseudomonas putida F1-genetic organization of the tod operon. Appl Environ Microbiol 54(6): 1498-1503.
30. Zylstra GJ, Wackett LP, Gibson DT (1989) Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes. Appl Environ Microbiol 55(12): 3162-3166.