Purification, cloning, and overexpression of an alcohol dehydrogenase from Nocardia globerula reducing aliphatic ketones and bulky ketoesters

Applied Microbiology and Biotechnology

Volumn 86, Issue 6, 2010, Pages 1813-1820

  Parkot, Julia ^a,c   Gröger, Harald ^b,d   Hummel, Werner ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b EVONIK INDUSTRIES AG   (Germany)

^c Jennewein Biotechnologie GmbH   (Germany)

^d UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

3 Hydroxyacyl CoA dehydrogenase; Alcohol dehydrogenase; Biocatalysis; Nocardia globerula

Indexed keywords

ACETOPHENONES; ALCOHOL DEHYDROGENASE; ALIPHATIC KETONES; BACTERIAL SOURCE; BIOCATALYSIS; CHIRAL CHEMICALS; GEL FILTRATION; GENOME SEQUENCING; KETO ESTER; NOCARDIA; OVER-EXPRESSION; TETRAMERS; THIOESTERS; WILD TYPES;

CLONING; ENANTIOSELECTIVITY; ESCHERICHIA COLI; ESTERS; GELS; KETONES; MAMMALS; PURIFICATION;

ENZYMES;

3 OXOBUTANOIC ACID TERT BUTYLTHIOESTER; 4 CHLORO 3 HYDROXY BUTANOATE; ACETOPHENONE; ALCOHOL DEHYDROGENASE; ALIPHATIC KETONE; BUTYRIC ACID; ESTER DERIVATIVE; KETOESTER; UNCLASSIFIED DRUG;

ALCOHOL; BACTERIUM; BIOTRANSFORMATION; CATALYSIS; ENZYME ACTIVITY; ESTER; GENE EXPRESSION; GENOME; KETONE; MAMMAL;

ARTICLE; BIOCATALYST; ENANTIOSELECTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; GEL FILTRATION; GENE SEQUENCE; MOLECULAR CLONING; NOCARDIA; NOCARDIA GLOBERULA; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION;

ALCOHOL DEHYDROGENASE; AMINO ACID SEQUENCE; BASE SEQUENCE; BIOCATALYSIS; BIOTRANSFORMATION; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; ESTERS; KETONES; MOLECULAR SEQUENCE DATA; NOCARDIA; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; NOCARDIA GLOBERULA;

EID: 77952879641     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2385-x     Document Type: Article

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