Identification of cross-contaminated animal cells by PCR and isoenzyme analysis

Cytotechnology

Volumn 61, Issue 3, 2009, Pages 81-92

  Ramya, R ^a   Nagarajan, T ^a   Sivakumar, V ^a   Senthilkumar, R L ^a   Bala Obulapathi, B ^a   Thiagarajan, D ^a   Srinivasan, V A ^a  

^a Indian Immunologicals Limited   (India)

Author keywords

Cross contamination; Cyt b gene; Isoenzyme analysis; Nested PCR

Indexed keywords

CYTOCHROME B; MITOCHONDRIAL DNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; ISOENZYME ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION;

ANIMALIA;

EID: 77952878680     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-009-9245-5     Document Type: Article

References (32)

1. S Coecke M Balls G Bowe J Davis G Gstraunthaler T Hartung R Hay OW Merten A Price L Schechtman G Stacey W Stokes 2005 Guidance on good cell culture practice. A report of the second ECVAM task force on good cell culture practice Altern Lab Anim 33 261 287 1:CAS:528:DC%2BD2MXmsVart7o%3D
2. WG Dirks RAF MacLeod K Jaeger H Milch HG Drexler 1999 First searchable data base for DNA profile of human cell lines; sequential use of fingerprint techniques for authentication Cell Mol Biol 45 841 853 1:CAS:528: DyaK1MXmvFenu7w%3D
3. MNE Forsell Y Li M Sundback K Svehla P Liljestorm JR Masera R Wyatt GBK Hedestam 2005 Biochemical and immunogenic characterization of soluble human immunodeficiency virus type 1 envelope glycoprotein trimers expressed by Semliki Forest virus J Virol 79 10902 10914 10.1128/JVI.79.17.10902-10914.2005 1:CAS:528:DC%2BD2MXps1Gls7w%3D (Pubitemid 41170632)
4. RI Freshney 2002 Cell line provenance Cytotechnology 39 55 67 10.1023/A;1022949730029 10.1023/A:1022949730029 1:CAS:528:DC%2BD3sXisVWiur4%3D
5. Freshney RI (2006) Culture of cells for tissue engineering. Wiley, Hoboken
6. SM Gartler 1967 Genetic markers as tracers in cell culture Natl Cancer Inst Monogr 26 167 195 1:STN:280:DyaF1c%2FptVGnsQ%3D%3D
7. DM Halton WD Peterson Jr B Hukku 1983 Cell culture quality control by rapid isoenzymatic characterization In Vitro 19 16 24 10.1007/BF02617989 1:CAS:528:DyaL3sXht1ansrk%3D
8. Hay RJ (2000) Animal cell culture, a practical approach. IRL Press, Oxford
9. Hay RJ (2002) Methods of tissue engineering. Academic Press, New York
10. AJ Jeffreys V Wilson SL Thein 1985 Individual-specific 'fingerprints' of human DNA Nature 316 76 79 10.1038/316076a0 10.1038/316076a0 1:CAS:528:DyaL2MXkvF2ltrc%3D (Pubitemid 15013718)
11. Keith WN (2000) Animal cell culture, a practical approach. IRL Press, Oxford
12. MY Liu SC Lin H Liu F Candal A Vafai 2003 Identification and authentication of animal cell culture by polymerase chain reaction amplification and DNA sequencing In Vitro Cell Dev Biol Anim 39 424 427 10.1290/1543- 706X(2003)039<0424:IAAOAC>2.0.CO;2 1:CAS:528:DC%2BD2cXkslOmsro%3D (Pubitemid 38657499)
13. M Lopez-Andreo L Lugo A Garrido-Pertierra MI Prieto A Puyet 2005 Identification and quantitation of species in complex DNA mixtures by real time polymerase chain reaction Anal Biochem 339 73 82 10.1016/j.ab.2004.11.045 10.1016/j.ab.2004.11.045 1:CAS:528:DC%2BD2MXit1eqsLw%3D (Pubitemid 40342681)
14. GC Losi S Ferrari E Sossi R Villa M Ferrari 2008 An alternative method to isoenzyme profile for cell line identification and interspecies cross-contaminations: cytochrome b PCR-RFLP analysis In Vitro Cell Dev Biol Animal 44 321 329 10.1007/S11626-008-9125-X 10.1007/s11626-008-9125-x 1:CAS:528:DC%2BD1cXhsFWkt7%2FK
15. O Markovic N Markovic 1998 Cell cross-contamination in cell cultures: the silent and neglected danger In Vitro Cell Dev Biol Anim 34 1 8 10.1007/S11626-998-0040-Y 10.1007/s11626-998-0040-y 1:CAS:528:DyaK1cXht12jsb0%3D
16. JR Masters 2000 Human cancer cell lines: facts and fantasy Nat Rev Mol Cell Biol 1 233 236 10.1038/35043102 1:CAS:528:DC%2BD3MXivVGjsbo%3D
17. OJ Miller DA Miller PW Allderdice VG Dev MS Grewal 1971 Quinacrine fluorescent karyotypes of human diploid and heteroploid cell lines Cytogenetics 10 338 346 10.1007/BF02633971 10.1159/000130152 1:STN:280:DyaE387gtVGnsg%3D%3D
18. W Nelson-Rees RR Flandermeyer 1977 Inter-and intraspecies contamination of human breast tumor cell lines HBC and BrCa5 and other cell cultures Science 195 1343 1344 10.1126/science.557237 1:STN:280:DyaE2s7islehsw%3D%3D
19. WA Nelson-Rees RR Flandermeyer PK Hawthorne 1974 Banded marker chromosome as indicators of intraspecies cellular contamination Science 184 1093 1096 10.1126/Science.184.4141.1093 10.1126/science.184.4141.1093 1:STN:280: DyaE287ivFOjtw%3D%3D
20. WA Nelson-Rees DW Daniels RR Flandermeyer 1989 Contamination of cells in culture Science 212 446 452 10.1126/science.6451928
21. RW Nims CJ Herbstritt 2005 Cell line authentication using isoenzyme analysis BioPharm Int 1 1 5
22. RW Nims AP Shoemaker MA Bauernschub LJ Rec JW Harbell 1998 Sensitivity of isoenzyme analysis for the detection of interspecies cell line cross-contamination In Vitro Cell Dev Biol Anim 34 35 39 10.1007/s11626-998- 0050-9 1:CAS:528:DyaK1cXht12jtrw%3D
23. SJ O'Brien JE Shannon MH Gail 1980 A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures In Vitro 16 119 135 10.1007/BF02831503 10.1007/BF02831503
24. CM O'Toole S Pavey P Hepburn LM Frank 1983 Identity of some human bladder cancer lines Nature 201 429 430 10.1038/301429a0 (Pubitemid 13160559)
25. K Ono M Satoh T Yoshida Y Ozawa A Kohara M Takeuchi 2007 Species identification of animal cells by nested PCR targeted to mitochondrial DNA In Vitro Cell Dev Biol Anim 10.1007/S11626-007-9033-5 (Pubitemid 350104809)
26. B Parodi O Aresu D Bini R Lorenzini F Schena P Visconti, et al. 2002 Species identification and confirmation of human and animal cell lines: a PCR-based method Biotechniques 32 432 440 1:CAS:528:DC%2BD38Xht1ChsLo%3D
27. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
28. GN Stacey BJ Bolton A Doyle 1992 DNA fingerprinting transforms the art of cell authentication Nature 357 261 262 10.1038/35726/a0 10.1038/357261a0 1:STN:280:DyaK383mvF2nsQ%3D%3D
29. GN Stacey H Hoelzl JR Stephenson A Doyle 1997 Authentication of animal cell cultures by direct visualization of repetitive DNA, aldolase gene PCR and isoenzyme analysis Biologicals 25 75 85 10.1006/biol.1996.0062 10.1006/biol.1996.0062 1:CAS:528:DyaK2sXjvVyjtb4%3D (Pubitemid 27230520)
30. GN Stacey JR Masters RJ Hay HG Drexler RAF MacLeod RI Freshney 2000 Cell contamination leads to inaccurate data: we must take action now Nature 403 356 10.1038/35000394 1:CAS:528:DC%2BD3cXps1amtg%3D%3D
31. KG Steube D Grunicke HG Drexler 1995 Isoenzyme analysis as a rapid method for the examination of the species identity of cell cultures In Vitro Cell Dev Biol Anim 31 115 119 10.1007/BF02633971 10.1007/BF02633971 1:CAS:528: DyaK2MXltlCrurc%3D
32. CS Stulberg WR Peterson Jr WF Simpson 1976 Identification of cells in culture Am J Hematol 1 237 242 10.1002/ajh.2830010208 1:STN:280: DyaE2s%2FmtlWgsw%3D%3D