Decreasing Txnip mRNA and protein levels in pancreatic MIN6 cells reduces reactive oxygen species and restores glucose regulated insulin secretion

Cellular Physiology and Biochemistry

Volumn 25, Issue 6, 2010, Pages 667-674

  Rani, Sweta ^a   Mehta, Jai Prakash ^b   Barron, Niall ^c   Doolan, Padraig ^c   Jeppesen, Per Bendix ^d   Clynes, Martin ^c   O'Driscoll, Lorraine ^a  

^a TRINITY COLLEGE DUBLIN   (Ireland)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

^c DUBLIN CITY UNIVERSITY   (Ireland)

^d AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

cDNA; Glucose stimulated insulin secretion; Immunoprecipitation; ROS; ShRNA; SiRNA; Txnip

Indexed keywords

COMPLEMENTARY DNA; GLUCOSE; INSULIN; MESSENGER RNA; REACTIVE OXYGEN METABOLITE; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; THIOREDOXIN INTERACTING PROTEIN; THIOREDOXIN;

ARTICLE; CONTROLLED STUDY; DIABETES MELLITUS; ENZYME LINKED IMMUNOSORBENT ASSAY; HORMONAL REGULATION; IMMUNOPRECIPITATION; INSULIN RELEASE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TARGETING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING; ANIMAL; CELL LINE; CELL PROLIFERATION; CYTOLOGY; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; METABOLISM; PANCREAS; PANCREAS ISLET BETA CELL;

ANIMALS; CELL LINE; CELL PROLIFERATION; GENE EXPRESSION REGULATION; GENE KNOCKDOWN TECHNIQUES; GLUCOSE; INSULIN; INSULIN-SECRETING CELLS; PANCREAS; REACTIVE OXYGEN SPECIES; RNA, MESSENGER; RNA, SMALL INTERFERING; THIOREDOXINS;

EID: 77952741810     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000315086     Document Type: Article

References (19)

1. Chen KS, DeLuca HF: Isolation and characterization of a novel cdna from hl-60 cells treated with 1,25-dihydroxyvitamin d-3. Biochim Biophys Acta 1994;1219:26-32.
2. Han SH, Jeon JH, Ju HR, Jung U, Kim KY, Yoo HS, Lee YH, Song KS, Hwang HM, Na YS, Yang Y, Lee KN, Choi I: Vdup1 upregulated by tgf-beta1 and 1,25-dihydorxyvitamin d3 inhibits tumor cell growth by blocking cell-cycle progression. Oncogene 2003;22:4035-4046.
3. Sheth SS, Bodnar JS, Ghazalpour A, Thipphavong CK, Tsutsumi S, Tward AD, Demant P, Kodama T, Aburatani H, Lusis AJ: Hepatocellular carcinoma in txnip-deficient mice. Oncogene 2006;25:3528-3536.
4. Chutkow WA, Patwari P, Yoshioka J, Lee RT: Thioredoxin-interacting protein (txnip) is a critical regulator of hepatic glucose production. J Biol Chem 2008;283:2397-2406.
5. Yoshioka J, Imahashi K, Gabel SA, Chutkow WA, Burds AA, Gannon J, Schul-ze PC, MacGillivray C, London RE, Murphy E, Lee RT: Targeted deletion of thioredoxin-interacting protein regulates cardiac dysfunction in response to pressure overload. Circ Res 2007;101:1328-1338.
6. Oka S, Liu W, Masutani H, Hirata H, Shinkai Y, Yamada S, Yoshida T, Nakamura H, Yodoi J: Impaired fatty acid utilization in thioredoxin binding protein-2 (tbp-2)-deficient mice: A unique animal model of reye syndrome. FASEB J 2006;20:121-123.
7. Lee KN, Kang HS, Jeon JH, Kim EM, Yoon SR, Song H, Lyu C Y, Piao ZH, Kim SU, Han YH, Song SS, Lee YH, Song KS, Kim YM, Yu DY, Choi I: Vdup1 is required for the development of natural killer cells. Immunity 2005;22:195-208.
8. Parikh H, Carlsson E, Chutkow WA, Johansson LE, Storgaard H, Poulsen P, Saxena R, Ladd C, Schulze PC, Mazzini MJ, Jensen CB, Krook A, Bjornholm M, Tornqvist H, Zierath JR, Ridderstrale M, Altshuler D, Lee RT, Vaag A, Groop LC, Mootha VK: Txnip regulates peripheral glucose metabolism in humans. PLoS Med 2007;4:e158.
9. Li X, Rong Y, Zhang M, Wang XL, LeMaire SA, Coselli JS, Zhang Y, Shen YH: Up-regulation of thioredoxin interacting protein (txnip) by p38 mapk and foxo1 contributes to the impaired thioredoxin activity and increased ros in glucose-treated endothelial cells. Biochem Biophys Res Commun 2009;381:660-665.
10. Gokulakrishnan K, Mohanavalli KT, Monickaraj F, Mohan V, Balasubrama-nyam M: Subclinical inflammation/oxidation as revealed by altered gene expression profiles in subjects with impaired glucose tolerance and type 2 diabetes patients. Mol Cell Biochem 2009;324:173-181.
11. Minn AH, Hafele C, Shalev A: Thioredoxin-interacting protein is stimulated by glucose through a carbohydrate response element and induces beta-cell apoptosis. Endo crinology 2005;146:2397-2405.
12. Chen J, Saxena G, Mungrue IN, Lusis AJ, Shalev A: Thioredoxin-interacting protein: A critical link between glucose toxicity and beta-cell apoptosis. Diabetes 2008;57:938-944.
13. Shaked M, Ketzinel-Gilad M, Ariav Y, Cerasi E, Kaiser N, Leibowitz G: Insulin counteracts glucotoxic effects by suppressing thioredoxin-interacting protein production in ins-1e beta cells and in psammomys obesus pancreatic islets. Diabetologia 2009;52:636-644.
14. Cha-Molstad H, Saxena G, Chen J, Shalev A: Glucose-stimulated expression of txnip is mediated by carbohydrate response element-binding protein, p300, and histone h4 acetylation in pancreatic beta cells. J Biol Chem 2009;284:16898-16905.
15. Miyazaki J, Araki K, Yamato E, Ikegami H, Asano T, Shibasaki Y, Oka Y, Yamamura K: Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: Special reference to expression of glucose transporter isoforms. Endocrinology 1990;127:126-132.
16. Lilla V, Webb G, Rickenbach K, Maturana A, Steiner DF, Halban PA, Irminger JC: Differential gene expression in well-regulated and dysregulated pancreatic beta-cell (min6) sublines. Endocrinology 2003;144:1368-1379.
17. Dowling P, O'Driscoll L, O'Sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M: Proteomic screening of glucose-responsive and glucose non-responsive min-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress. Proteomics 2006;6:6578-6587.
18. O'Driscoll L, Gammell P, Clynes M: Mechanisms associated with loss of glucose responsiveness in beta cells. Transplant Proc 2004;36:1159-1162.
19. O'Driscoll L, Gammell P, McKiernan E, Ryan E, Jeppesen PB, Rani S, Clynes M: Phenotypic and global gene expression profile changes between low passage and high passage min-6 cells. J Endocrinol 2006;191:665-676.