Expression, purification of the UL3 protein of herpes simplex virus type 1, and production of UL3 polyclonal antibody

Journal of Virological Methods

Volumn 166, Issue 1-2, 2010, Pages 72-76

  Lin, Fusen ^a   Ren, Xiaoming ^b   Guo, Hong ^a   Ding, Qiong ^a   Zheng, Alan C ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b BEIJING UNIVERSITY OF AGRICULTURE   (China)

Author keywords

Herpes simplex virus type 1 (HSV 1); Immunofluorescent assay; Polyclonal antibody; Protein expression; Protein purification; UL3 protein

Indexed keywords

DODECYL SULFATE SODIUM; NICKEL; POLYCLONAL ANTIBODY; POLYCLONAL ANTIBODY UL3; PROTEIN UL3; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AFFINITY CHROMATOGRAPHY; ANTIBODY DETECTION; ANTIBODY PRODUCTION; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CONSTRUCT; HERPES SIMPLEX VIRUS 1; IMMUNOFLUORESCENCE TEST; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, VIRAL; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HERPESVIRUS 1, HUMAN; MICROSCOPY, FLUORESCENCE; PLASMIDS; RABBITS; RECOMBINANT FUSION PROTEINS; VIRAL PROTEINS;

ANIMALIA; ESCHERICHIA COLI; HUMAN HERPESVIRUS 1; PROKARYOTA;

EID: 77952671702     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.02.022     Document Type: Article

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