Assessment of chemical effects on aromatase activity using the H295R cell line

Environmental Science and Pollution Research

Volumn 17, Issue 5, 2010, Pages 1137-1148

  Higley, Eric B ^a   Newsted, John L ^b   Zhang, Xiaowei ^a   Giesy, John P ^a,c,d,e,f   Hecker, Markus ^a,b  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b ENTRIX INC   (United States)

^c CITY UNIVERSITY OF HONG KONG   (Hong Kong)

^d MICHIGAN STATE UNIVERSITY   (United States)

^e NANJING TECH UNIVERSITY   (China)

^f XIAMEN UNIVERSITY   (China)

Author keywords

Aminoglutethimide; Atrazine; Compensation; CYP19; Endocrine; Estradiol; Forskolin; Hormone; Ketoconazole; Letrozole; Prochloraz; Prometon; Steroidogenesis; Testosterone

Indexed keywords

CHEMICAL COMPOUND; DOSE-RESPONSE RELATIONSHIP; ECOSYSTEM HEALTH; ENDOCRINE SYSTEM; ENZYME ACTIVITY; PUBLIC HEALTH; SEX HORMONE; TOXICITY TEST;

EID: 77952671420     PISSN: 09441344     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11356-009-0285-3     Document Type: Article

References (37)

1. Almeida MQ, Brito VN, Linst TS, Guerra-Junior G, Castro M, Antonini SR, Arnhold IJP, Mendonca BB, Latronico AC (2008) Long-term treatments of familial male-limited precocious puberty (testotoxicosis) with cyproterone acetate of ketoconazole. Clin Endocrinol 69: 93-98.
2. Ankley G, Mihaich E, Stahl R, Tillitt D, Colborn T, McMaster S, Miller R, Bantle J, Campbell P, Denslow N, Dickerson R, Folmar L, Fry M, Giesy J, Gray LE, Guiney P, Hutchinson T, Kennedy S, Kramer V, LeBlanc G, Mayes M, Nimrod A, Patino R, Peterson R, Purdy R, Ringer R, Thomas P, Touart L, van Der Kraak G, Zacharewski T (1998) Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife. Environ Toxicol Chem 17: 68-87.
3. Ayub M, Stitch S (1986) Effect of ketoconazole on placental aromatase, 3-beta-hydroxysteroid dehydrogenase-isomerase and 17-beta-hydroxysteroid dehydrogenase. J Steroid Biochem Mol Bio 25: 981-984.
4. Blystone C, Lambright C, Howdeshell K, Furr J, Sternberg R, Butterworth B, Durhan E, Makynen E, Ankley G, Wilson V, LeBlanc G, Gray E (2007) Sensitivity of fetal rat testicular steroidogenesis to maternal prochloraz exposure and the underlying mechanism of inhibition. Toxicol Sci 97(2): 512-519.
5. Brooks J, Thompson L (2005) Mammalian ligands and genistein decrease the activities of aromatase and 17β-hydroysteroid dehydrogenase in MCF-7 cells. J Steroid Biochem Mol Bio 94: 461-467.
6. Fassnacht M, Beuschlein F, Vay S, Mora P, Allolio B, Reincke M (1998) Aminoglutethimide suppresses adrenocorticotropin receptor expression in the NCI-h295 adrenocortical tumor cell line. J Endocrinol 159: 35-42.
7. Foster A, Jarman M, Leung C, Rowlands M, Taylor G (1983) Analogs of aminoglutethimide-selective-inhibition of cholesterol side-chain cleavage. J Med Chem 26: 50-54.
8. Gazdar AF, Oie HK, Shackleton CH, Chen TR, Triche TJ, Myers CE, Chrousos GP, Brennan MF, Stein CA, La Rocca RV (1990) Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res 50: 5488-5496.
9. Gracia T, Hilscherova K, Jones P, Newsted J, Zhang X, Hecker M, Higley E, Sanderson J, Yu R, Wu R, Giesy J (2006) The H295R system for evaluation of endocrine-disrupting effects. Ecotoxicol Environ Saf 65: 293-305.
10. Hecker M, Giesy JP (2008) Novel trends in endocrine disruptor testing: the H295R steroidogenesis assay for identification of inducers and inhibitors of hormone production. Anal Bioanal Chem 390: 287-291.
11. Hecker M, Park J-W, Murphy M, Jones P, Solomon K, van Der Kraak G, Carr J, Smith E, du Preez L, Kendall R, Giesy JP (2005) Effects of atrazine on CYP19 gene expression and aromatase activity in testes and on plasma sex steroid concentrations of male African clawed frogs (Xenopus laevis). Toxicol Sci 86(2): 273-280.
12. Hecker M, Newsted J, Murphy M, Higley E, Jones P, Wu R, Giesy J (2006) Human adrenocarcinoma (H295R) cells for rapid in vitro determination of effect on steroidogenesis: hormone production. Toxicol Appl Pharmacol 217: 114-124.
13. Hecker M, Hollert H, Cooper R, Vinggard A-M, Akahori Y, Murphy M, Nellemann C, Higley E, Newsted J, Wu R, Lam P, Laskey J, Buckalew A, Grund S, Nakai M, Timm G, Giesy J (2007) The OECD validation program of the H295R steroidogenesis assay for the identification of in vitro inhibitors and inducers of testosterone and estradiol production, phase 2: inter-laboratory pre-validation studies. Env Sci Pollut Res 14(1): 23-30.
14. Hilscherova K, Jones PD, Gracia T, Newsted JL, Zhang XW, Sanderson JT, Yu RMK, Wu RSS, Giesy JP (2004) Assessment of the effects of chemicals on the expression of ten steroidogenic genes in the H295R cell line using real-time PCR. Toxicol Sci 81: 78-89.
15. Jobling S, Williams R, Johnson A, Taylor A, Gross-Sorokin M, Nolan M, Tyler C, van Aerle R, Santos E, Brighty G (2006) Predicted exposures to steroid estrogens in U. K. Rivers correlate with widespread sexual disruption in wild fish populations. Environ Health Perspect 114(1): 32-39.
16. Kumru S, Yildiz A, Yilmaz B, Sandal S, Gurates B (2007) Effects of aromatase inhibitors letrozole and anastrazole on bone metabolism and steroid hormone levels in intact female rats. Gynecol Endocrinol 23(10): 556-561.
17. Laier P, Metzdorff SB, Borch J, Hagen ML, Hass U, Christiansen S, Axelstad M, Kledal T, Dalgarrd M, McKinnell C, Brokken L, Vinggaard AM (2006) Mechanisms of action underlying the antiandrogenic effects of the fungicide prochloraz. Toxicol Appl Pharmacol 213: 160-171.
18. Laignelet L, Narbonne J-F, Lhuguenot J-C, Riviere J-L (1989) Induction and inhibition of rat liver cytochrome(s) P-450 by an imidiazole fungicide (prochloraz). Toxicology 59: 271-284.
19. Lephart E, Simpson E (1991) Assay of aromatase activity. Methods Enzymol 206: 477-483.
20. Loves S, Ruinemans-Koerts J, de Boer H (2008) Letrozole once a week normalizes serum testosterone in obesity-related male hypogonadism. Euro J Endo 158: 741-747.
21. Mosman T (1983) Rapid colometric assay for growth and survival: application to proliferation and cytotoxicity. J Immunol Methods 65: 55-63.
22. Naville D, Penhoat A, Durand P, Begeot M (1999) Three steroidogenic factor-1 binding elements are required for constitutive and cAMP regulated expression of the human adrenocorticotropin receptor gene. Biochem Biophys Res Commun 255: 28-33.
23. Rainey WE, Bird IM, Sawetawan C, Hanley NA, Mccarthy JL, Mcgee EA, Wester R, Mason JI (1993) Regulation of human adrenal carcinoma cell (Nci-H295) production of C19 steroids. J Clin Endocrinol Metab 77: 731-737.
24. Ryan KJ (1982) Biochemistry of aromatase-significance to female reproductive physiology. Cancer Res 42: 3342-3344.
25. Sanderson JT (2006) The steroid hormone biosynthesis pathway as a target for endocrine-disrupting chemicals. Toxicol Sci 94(1): 3-21.
26. Sanderson JT, Letcher R, Heneweer M, Giesy J, van den Berg M (2001) Effects of chloro-s-triazine herbicides and metabolites on aromatase activity in various cell lines and on vitellogenin production in male carp hepatocytes. Environ Health Perspect 109: 1027-1031.
27. Sanderson JT, Boerma J, Lansbergen G, van den Berg M (2002) Induction and inhibition of aromatase (CYP19) activity by various classes of pesticides in H295R human adrenocortical carcinoma cells. Toxicol Appl Pharmacol 182: 44-54.
28. Seamon KB, Padgett W, Daly JW (1981) Forskolin-unique diterpene activator of adenylate-cyclase in membranes and in intact-cells. Proc Natl Acad Sci 78: 3363-3367.
29. Simpson E, Clyne C, Rubin G, Boon W, Robertson K, Britt K, Speed C, Jones M (2002) Aromatase-a brief overview. Annu Rev Physiol 64: 93-127.
30. Staels B, Hum DW, Miller WL (1993) Regulation of steroidogenesis in NCI-H295 cells: a cellular model of the human fetal adrenal. Mol Endocrinol 7: 423-433.
31. Sumpter J, Johnson A (2005) Lessons from endocrine disruption and their application to other issues concerning trace organics in the aquatic environment. Environ Sci Technol 39(12): 4321-4332.
32. Ung D, Nager S (2009) Trans-resveratrol-mediated inhibition of β-estradiol conjugation in MCF-7 cells stably expressing human sulfotransferases SULT1A1 or SULT1E1, and human liver mirosomes. Xenobiotica 39(1): 72-79.
33. Villeneuve D, Murphy M, Kahl M, Jensen K, Butterworth B, Makynen E, Durhan E, Linnum A, Leino R, Curtis L, Giesy J, Ankley G (2006) Evaluation of the methoxytriazine herbicide prometon using a short-term fathead minnow reproduction test and a suite of in vitro bioassays. Environ Toxicol Chem 25: 8: 2143-2153.
34. Villeneuve D, Ankley G, Makynen A, Blake L, Greene K, Higley E, Newsted J, Giesy J, Hecker M (2007) Comparison of fathead minnow ovary explant and H295R cell-based steroidogenesis assays for identifying endocrine-active chemicals. Ecotoxicol Environ Saf 68: 20-32.
35. Vinggaard AM, Christiansen S, Laier P, Poulsen ME, Breinholt V, Jarfelt K, Jacobsen H, Dalgaard M, Nellemann C, Hass U (2005) Perinatal exposure to the fungicide prochloraz feminizes the male rat offspring. Toxicol Sci 85: 886-897.
36. Watanabe M, Nakajin S (2004) Forskolin up-regulates aromatase (CYP19) activity and gene transcripts in the human adrenocortical carcinoma cell line H295R. J Endocrinol 180: 125-133.
37. Weber MM, Will A, Aldermann B, Engelhart D (1991) Effect of ketoconazole on human ovarian C17, 20-desmolase and aromatase. J Steroid Biochem Mol Biol 38: 213-218.