An improved strip FRAP method for estimating diffusion coefficients: Correcting for the degree of photobleaching

Journal of Microscopy

Volumn 238, Issue 3, 2010, Pages 240-253

  Yang, J ^a   KOhler K ^b   Davis, D M ^b   Burroughs, N J ^c  

^a QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

Bleached mass; Diffusion equation; Immobile fraction; Recovery curve; Series approximation; Strip bleaching

Indexed keywords

CELL CULTURE; CLEANING; DIFFUSION; FLUORESCENCE; IMAGE SEGMENTATION; PHOTOBLEACHING; PROTEINS; RECOVERY;

BLEACHED MASS; CURVE ANALYSIS; DIFFUSION EQUATIONS; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; IMMOBILE FRACTION; RECOVERY CURVES; REGION-OF-INTEREST; REGIONS OF INTEREST; SERIES APPROXIMATIONS; STRIP BLEACHING;

BLEACHING;

FLUORESCEIN ISOTHIOCYANATE; GLYCEROL; GREEN FLUORESCENT PROTEIN; IMMUNOGLOBULIN G; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; HLA C ANTIGEN; HLA-C*06 ANTIGEN; HYBRID PROTEIN;

ARTICLE; B LYMPHOCYTE; BLEACHING; CONTROLLED STUDY; DIFFUSION COEFFICIENT; ERROR; FLUORESCENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; VALIDATION PROCESS; CELL LINE; CELL MEMBRANE; CHEMISTRY; DIFFUSION; FLUORESCENCE MICROSCOPY; GENETICS; PROCEDURES; THEORETICAL MODEL;

CELL LINE; CELL MEMBRANE; DIFFUSION; GREEN FLUORESCENT PROTEINS; HLA-C ANTIGENS; HUMANS; MICROSCOPY, FLUORESCENCE; MODELS, THEORETICAL; PHOTOBLEACHING; RECOMBINANT FUSION PROTEINS;

EID: 77952662829     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03347.x     Document Type: Article

References (25)

1. Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E. Webb, W.W. (1976) Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16, 1055-1069.
2. Braekmans, K., Peeters, L., Sanders, N.N., De Smedt, S.C. Demeester, J. (2003) Three-dimensional fluorescence recovery after photobleaching with the confocal scanning laser microscope. Biophys. J. 85, 2240-2252.
3. Braekmans, K., Remaut, K., Vandenbroucke, R.E., Lucas, B., De Smedt, S.C. Demeester, J. (2007) Line FRAP with the confocal laser scanning microscope for diffusion measurements in small regions of 3-D samples. Biophys. J. 92, 2172-2183.
4. Braga, J., Desterro, J.M.P. Carmo-Fonseca, M. (2004) Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopes. Mol. Biol. Cell 15, 4749-4760.
5. Davis, D.M., Chiu, I., Fassett, M., Cohen, G.B., Mandelboim, O. Strominger, J.L. (1999) The human natural killer cell immune synapse. Proc. Nat. Acad. Sci. 96, 15062-15067.
6. Dushek, O. Coombs, D. (2008) Improving parameter estimation for cell surface FRAP data. J. Biochem. Biophys. Methods. 70, 1224-1231.
7. Dustin, M.L., Golan, D.E., Zhu, D.-M., Miller, J.M., Meier, W., Davies, E.A. van der Merwe, P.A. (1997) Low affinity interaction of human or rat T cell adhesion molecule CD2 with its ligand aligns adhering membranes to achieve high physiological affinity. J. Biol. Chem. 272, 30889-30898.
8. Ellenberg, J., Siggia, E.D., Moreira, J.E., Smith, C.L., Presley, J.F., Worman, H.J. Lippincott-Schwartz, J. (1997) Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis. J. Cell Biol. 138, 1193-1206.
9. Jonsson, P., Jonsson, M.P., Tegenfeldt, J.O. Hook, F. (2008) A method improving the accuracy of fluorescence recovery after photobleaching analysis. Biophys. J. 95, 5334-5348.
10. Kaufman, E.N. Jain, R.K. (1990) Quantification of transport and binding parameters using fluorescence recovery after photobleaching. Potential for in vivo applications. Biophys. J. 58, 873-885.
11. Kubitscheck, U., Kuckmann, O., Kues, T. Peters, R. (2000) Imaging and tracking of single GFP molecules in solution. Biophys. J. 78, 2170-2179.
12. Kubitscheck, U., Wedekind, P. Peters, R. (1994) Lateral diffusion measurement at high spatial resolution by scanning microphotolysis in a confocal microscope. Biophys. J. 67, 948-956.
13. Lubelski, A. Klafter, J. (2008) Fluorescence recovery after photobleaching: the case of anomalous diffusion. Biophys. J. 94, 4646-4653.
14. Mege, J.-L., Capo, C., Benoliel, A.-M., Foa, C., Galindo, R. Bongrand, P. (1986) Quantification of cell surface roughness; a method for studying cell mechanical and adhesive properties. J. Theor. Biol. 119, 147-160.
15. Mullineaux, C.W., Tobin, M.J. Jones, G.R. (1997) Mobility of photosynthetic complexes in thylakoid membranes. Nature 390, 421-424.
16. Seiffert, S. Oppermann, W. (2005) Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope. J. Microsc. 220, 20-30.
17. Smith, B.A. McConnell, H.M. (1978) Determination of molecular motion in membranes using periodic pattern photobleaching. Proc. Nat. Acad. Sci. 75, 2759-2763.
18. Soumpasis, D.M. (1983) Theoretical analysis of fluorescence photobleaching recovery experiments. Biophys. J. 41, 95-97.
19. Sprague, B.L., Pego, R.L., Stavreva, D.A. McNally, J.G. (2004) Analysis of binding reactions by fluorescence recovery after photobleaching. Biophys. J. 86, 3473-3495.
20. Tolentino, T.P., Wu, J., Zarnitsyna, V.I., Fang, Y., Dustin, M.L. Zhu, C. (2008) Measuring diffusion and binding kinetics by contact area FRAP. Biophys. J. 95, 920-930.
21. Tsay, T.T. Jacobson, K.A. (1991) Spatial Fourier analysis of video photobleaching measurements. Principles and optimization. Biophys. J. 60, 360-368.
22. Ulrich, M., Kappel, C., Beaudouin, J., Hezel, S., Ulrich, J. Eils, R. (2006) Tropical-parameter estimation and simulation of reaction-diffusion models based on spatio-temporal microscopy images. Bioinformatics 22, 2709-2710.
23. Vrljic, M., Nishimura, S.Y., Brasselet, S., Moerner, W.E. McConnell, H.M. (2002) Translational diffusion of individual class II MHC membrane proteins in cells. Biophys. J. 83, 2681-2692.
24. Weiss, M. (2004) Challenges and artifacts in Quantitative Photobleaching experiments. Traffic 5, 662-671.
25. Wu, J., Fang, Y., Zarnitsyna, V.I., Tolentino, T.P., Dustin, M.L. Zhu, C. (2008) A coupled diffusion-kinetics model for analysis of contact-area FRAP experiment. Biophys. J. 95, 910 919.