Detection of pathogenic Vibrio parahaemolyticus through biochemical and molecular-based methodologies in coastal waters of the Gulf of Trieste (North Adriatic Sea)

FEMS Microbiology Letters

Volumn 307, Issue 2, 2010, Pages 158-164

  Fabbro, Cinzia ^a   Cataletto, Bruno ^a   Del Negro, Paola ^a  

^a ISTITUTO NAZIONALE DI OCEANOGRAFIA E DI GEOFISICA SPERIMENTALE OGS   (Italy)

Author keywords

16S rRNA gene; Phenotypic based identification; Tdh and trh genes; ToxR and tlh genes; Vibrio parahaemolyticus

Indexed keywords

BACTERIAL PROTEIN; PROTEIN TLH; PROTEIN TOXR; RNA 16S; UNCLASSIFIED DRUG; DNA BINDING PROTEIN; TOXR PROTEIN, BACTERIA; TRANSCRIPTION FACTOR; VIRULENCE FACTOR;

ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; CONTROLLED STUDY; GENE AMPLIFICATION; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; VIBRIO PARAHAEMOLYTICUS; AGAR GEL ELECTROPHORESIS; BACTERIAL GENE; BACTERIUM IDENTIFICATION; GENETICS; ISOLATION AND PURIFICATION; ITALY; MICROBIOLOGY; PROCEDURES; SEA;

VIBRIO; VIBRIO PARAHAEMOLYTICUS;

BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; DNA-BINDING PROTEINS; ELECTROPHORESIS, AGAR GEL; GENES, BACTERIAL; ITALY; OCEANS AND SEAS; PHENOTYPE; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; TRANSCRIPTION FACTORS; VIBRIO PARAHAEMOLYTICUS; VIRULENCE FACTORS; WATER MICROBIOLOGY;

EID: 77952620749     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.01969.x     Document Type: Article

References (41)

1. Alsina M Blanch AR (1994a) A set of keys for biochemical identification of environmental Vibrio species. J Appl Bacteriol 76: 79-85. (Pubitemid 24205071)
2. Alsina M Blanch AR (1994b) Improvement and update of a set of keys for biochemical identification of environmental Vibrio species. J Appl Bacteriol 77: 719-721. (Pubitemid 24371578)
3. Altschul SF, Gish W, Miller W, Myers EW Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
4. Austin B Lee JV (1992) Aeromonadaceae and Vibrionaceae. Identification Methods in Applied and Environmental Microbiology (Board RG, Jones D Skinner FA, eds), pp. 163 182. Blackwell Scientific Publications
5. Austin B, Austin DA, Blanch AR et al 1997) A comparison of methods for the typing of fish-pathogenic Vibrio sp. Syst Appl Microbiol 20: 89-101.
6. Baumann L Baumann P (1981) The marine Gram negative eubacteria: genera Photobacterium, Becekea, Alteromonas, Pseudomonas, and Alcaligenes. The Prokaryotes, Vol. 2 (Starr MP, Stolp H, Truper HG, Balows A Scheglel HG, eds), pp. 516 550. Springer-Verlag
7. Bej AK, Patterson DP, Brasher CW, Vickery MCL, Jones DD Kaysner CA (1999) Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh. J Microbiol Meth 36: 215-225.
8. Caburlotto G, Ghidini V, Gennari M, Tafi MC Ileo MM (2008) Isolation of a Vibrio parahaemolyticus pandemic strain from a marine water sample obtained in the Northern Adriatic. Eurosurveillance 13 (1-3): 105-106.
9. Celussi M Cataletto B (2007) Annual dynamics of bacterioplankton assemblages in the Gulf of Trieste (Northern Adriatic Sea). Gene 406: 113-123.
10. Choopun N, Louis V, Huq A Colwell RR (2002) Simple procedure for rapid identification of Vibrio cholerae from the aquatic environment. Appl Environ Microb 68: 995-998.
11. Colodner R, Raz R, Meir I, Lazarovic T, Lerner L, Kopelowitz J, Keness Y, Sakran W, Ken-Dror S Bisharat N (2004) Identification of the emerging pathogen Vibrio vulnificus biotype 3 by commercially available phenotypic methods. J Clin Microbiol 42: 4137-4140.
12. Colwell RR (1984) Vibrios in the environment. Vibrios in the Environment (West PA Colwell RR, eds), pp. 1 634. John Wiley & Sons Inc.
13. Croci L, Serratore P, Cozzi L, Stacchini A, Milandri S, Suffredini E Toti L (2001) Detection of Vibrionaceae in mussels and in their seawater growing area. Microbiol Aliments Nutr 14: 161-165.
14. Croci L, Suffredini E The Vibrio parahaemolyticus Working Group et al 2007) Comparison of different biochemical and molecular methods for the identification of Vibrio parahaemolyticus. J Appl Microbiol 102: 229-237.
15. Dalsgaard A, Dalsgaard L, Høi L Larsen JL (1996) Comparison of a commercial biochemical kit and an oligonucleotide probe for identification of environmental isolates of Vibrio vulnificus. Lett Appl Microbiol 22: 184-188.
16. Daniels NA, MacKinnon L, Bishop R et al 2000) V. parahaemolyticus infection in the United States, 1973-1998. J Infect Dis 181: 1661-1666.
17. Deepanjali A, Sanath Kumar H, Karunasagar I Karunasagar I (2005) Seasonal variation in abundance of total and pathogenic Vibrio parahaemolyticus bacteria in oysters along the southwest coast of India. Appl Environ Microb 71: 3575-3580.
18. DePaola A, Hopkins LH, Peeler JT, Wentz B McPherson RM (1990) Incidence of Vibrio parahaemolyticus in United States coastal waters and oysters. Appl Environ Microb 56: 2299-2302.
19. DePaola A, Nordstrom JL, Bowers JC, Wells JC Cook DV (2003a) Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters. Appl Environ Microb 69: 1521-1526.
20. DePaola A, Ulaszek J, Kaysner CA, Tenge BJ, Nordstrom JL, Wells J, Puhur N Gendel SM (2003b) Molecular, serological and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food and clinical sources in North America and Asia. Appl Environ Microb 69: 3999-4005.
21. Don RH, Cox PT, Wainwright BJ, Baker K Mattick JS (1991) 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19: 4008.
22. Dorsch M, Lane D Stackebrandt E (1992) Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences. Int J Syst Bacteriol 42: 58-63.
23. Drake SL, De Paola A Jaykus LA (2007) An overview of Vibrio vulnificus and Vibrio parahaemolyticus. Compr Rev Food Sci F 6: 120-144.
24. Elliot EL, Kaysner AC, Jackson L Tamplin ML (2001) Vibrio cholerae, V. parahaemolyticus, V. vulnificus, and other Vibrio spp. USFDA Bacteriological Analytical Manual, 8th edn. pp. 901 927. AOAC International
25. Holt GJ, Krieg NR, Sneath PHA, Staley JT Williams ST (1994) Differentiation of Vibrio species and biovars. Bergey's Manual of Determinative Bacteriology (Hensyl WR, ed), pp. 260 274. Williams and Wilkins
26. Joseph SW, Colwell RR Kaper JB (1982) Vibrio parahaemolyticus and related halophilic Vibrios. Crit Rev Microbiol 10: 77-123.
27. Kaneko T Colwell RR (1975) Adsorption of Vibrio parahaemolyticus onto chitin and copepods. Appl Microbiol 29: 269-274.
28. Karunasagar I, Karunasagar I, Venugopal MN Nagesha CN (1987) Survival of Vibrio parahaemolyticus in estuarine water, seawater and in association with clams. Syst Appl Microbiol 9: 316-319.
29. Kim YB, Okuda J, Matsumoto C, Takahashi N, Hashimoto S Nishibuchi M (1999) Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J Clin Microbiol 37: 1173-1177.
30. Lane DJ (1991) 16S/23S rRNA sequencing. Nucleic Acid Techniques in Bacterial Systematics (Stackebrandt E Goodfellow M, eds), pp. 115 175. Wiley
31. Lemos ML, Toranzo AE Barja JL (1985) Modified medium for the oxidation-fermentation test 30 in the identification of marine bacteria. Appl Environ Microb 49: 1541-1543.
32. Martinez-Urtaza J, Lozano Leon A, De Paola A, Ishibashi M, Shimada K, Nishibuchi M Liebana E (2004) Characterization of pathogenic Vibrio parahaemolyticus isolates from clinical sources in Spain and comparison with Asian and North American pandemic isolates. J Clin Microbiol 42: 4672-4678.
33. O'Hara CM, Sowers EG, Bopp CA, Duda SB Strockbine NA (2003) Accuracy of six commercially available systems for identification of members of the family Vibrionaceae. J Clin Microbiol 41: 5654-5659.
34. Okuda J, Ishibashi M, Abbot SL, Janda JM Nishibuchi M (1997) Analysis of the thermostable direct hemolysin (tdh) gene and the tdh-related hemolysin (trh) genes in urease-positive strains of Vibrio parahaemolyticus isolated on the west coast of the United States. J Clin Microbiol 35: 1965-1971.
35. Ottaviani D, Masini L Bacchiocchi S (2003) A biochemical protocol for the isolation and identification of current species of Vibrio in seafood. J Appl Microbiol 95: 1277-1284.
36. Ottaviani D, Santarelli S, Bachoicchi S, Masini L, Ghittino C Bachiocchi I (2005) Presence of pathogenic Vibrio parahaemolyticus strains in mussels from the Adriatic Sea, Italy. Food Microbiol 22: 585-590.
37. Ottaviani D, Leoni F, Rocchegiani E, Santarelli S, Canonico C, Masini L, DiTrani V Carraturo A (2008) First clinical report of pandemic Vibrio parahaemolyticus 03: K6 infection in Italy. J Clin Microbiol 46: 2144-2145.
38. Thompson FL, Tetsuya I Swings J (2004) Biodiversity of vibrios. Microbiol Mol Biol R 68: 403-431.
39. Toti L, Croci L, Serratore P, Stacchini A, Milandri S Cozzi L (1996) Bacteria isolated from seawater and mussels: identification and toxin production. Lett Appl Microbiol 32: 57-61.
40. Wong HC, Chen MC, Liu SH Liu DP (1999) Incidence of highly genetically diversified Vibrio parahaemolyticus in seafood imported from Asia. Int J Food Microbiol 52: 181-188.
41. Yang Z, Jiao X, Zhou X, Cao G, Fang W Gu R (2008) Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China. Int J Food Microbiol 125: 279 285.