Regularized phase tomography enables study of mineralized and unmineralized tissue in porous bone scaffold

Journal of Microscopy

Volumn 238, Issue 3, 2010, Pages 230-239

  Langer, M ^a,b   Liu, Y ^c   Tortelli, F ^c   Cloetens, P ^a   Cancedda, R ^c   Peyrin, F ^a,b  

^a EUROPEAN SYNCHROTRON RADIATION FACILITY   (France)

^b UNIVERSITÉ DE LYON   (France)

^c UNIVERSITY OF GENOA   (Italy)

Author keywords

Bioceramic scaffold; Bone tissue engineering; Microtomography; Phase imaging; Synchrotron radiation

Indexed keywords

BONE; COMPUTER ARCHITECTURE; COMPUTERIZED TOMOGRAPHY; OSTEOBLASTS; SCAFFOLDS (BIOLOGY); SYNCHROTRONS; THICKNESS MEASUREMENT;

BIOCERAMIC SCAFFOLDS; BONE TISSUE ENGINEERING; FIBROUS MATRIX; IN-VITRO; MICRO-TOMOGRAPHY; PHASE IMAGING; PHASE TOMOGRAPHY; POROUS BONE SCAFFOLD; SKELITE; VITRO CELL;

SYNCHROTRON RADIATION;

ANIMAL CELL; ARTICLE; BONE; BONE MINERALIZATION; BONE SCAFFOLD; BONE STRUCTURE; CALCIFICATION; CELL CULTURE; COMPUTER ASSISTED TOMOGRAPHY; CONTROLLED STUDY; HISTOLOGY; HOLOGRAPHY; IMAGE RECONSTRUCTION; IN VITRO STUDY; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; OSTEOBLAST; OSTEOCLAST; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REGULARIZED PHASE TOMOGRAPHY; SYNCHROTRON RADIATION; THICKNESS; THREE DIMENSIONAL IMAGING; TOMOGRAPHY; BONE DEVELOPMENT; HUMAN; MICRO-COMPUTED TOMOGRAPHY; ORGAN CULTURE TECHNIQUE; PROCEDURES; ULTRASTRUCTURE;

BONE AND BONES; BONE DEVELOPMENT; HUMANS; ORGAN CULTURE TECHNIQUES; OSTEOBLASTS; OSTEOCLASTS; X-RAY MICROTOMOGRAPHY;

EID: 77952612952     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03345.x     Document Type: Article

References (39)

1. Bernhardt, R., Scharnweber, D., Müller, B., et al. H. (2004) Comparison of microfocus-and synchrotron X-ray tomography for the analysis of osteointegration around ti6al4v-implants. Eur. Cell. Mater. 7, 42 51.
2. Bernhardt, R., van den Dolder, J., Bierbaum, S., et al 2005) Osteoconductive modifications of Ti-implants in a goat defect model: characterization of bone growth with SR μCT and histology. Biomaterial 26, 3009 3019.
3. Cancedda, R., Cedola, A., Giuliani, A., et al 2007) Bulk and interface investigations of scaffolds and tissue-engineered bones by X-ray microtomography and X-ray microdiffraction. Biomaterial 28, 2505 2524.
4. Cartmell, S., Huynh, K., Lin, A., Nagaraja, S. Guldberg, R. (2003) Quantitative microcomputed tomography analysis of mineralization within three-dimensional scaffolds in vitro. J. Biomed. Mater. Res. A 69, 97 104.
5. Cloetens, P., Barrett, R., Baruchel, J., Guigay, J.P. Schlenker, M. (1996) Phase objects in synchrotron radiation hard X-ray imaging. J. Phys. D.: Appl. Phys. 29, 133 146.
6. Cloetens, P., Ludwig, W., Baruchel, J., Van Dyck, D., Van Landuyt, J., Guigay, J.P. Schlenker, M. (1999) Holotomography: quantitative phase tomography with micrometer resolution using hard synchrotron radiation X rays. Appl. Phys. Lett. 75, 2912 2914.
7. Cloetens, P., Mache, R., Schlenker, M. Lerbs-Mache, S. (2006) Quantitative phase tomography of Arabidopsis seeds reveals intercellular void network. PNAS 103, 14626 14630.
8. Glazer, P.A., Spencer, U.M., Alkalay, R.N. Schwardt, J. (2001) In vivo evaluation of calcium sulfate as a bone graft substitute for lumbar spinal fusion. Spine J. 1, 395 401.
9. Guigay, J. P. (1977) Fourier transform analysis of Fresnel diffraction patterns and in-line holograms. Optik 46, 121 125.
10. Guigay, J.P., Langer, M., Boistel, R. Cloetens, P. (2007) Mixed contrast transfer and transport of intensity approach for phase retrieval in the Fresnel region. Opt. Lett. 32, 1617 1619.
11. Gureyev, T.E., Paganin, D.M., Myers, G.R., Nesterets, Y.I. Wilkins, S.W. (2006) Phase-and-amplitude computer tomography. Appl. Phys. Lett. 89, 034-102.
12. Hansen, P.C. (1992) Analysis of discrete ill-posed problems by means of the L-curve. SIAM Rev. 34, 561 580.
13. Hansen, P.C. (2001) The L-curve and its use in the numerical treatment of inverse problems. Computational Inverse Problems in Electrocardiology (ed. by P. Jonston pp. 119 142. WIT Press
14. Hildebrand, T. Rüegsegger, P. (1997) A new method for the model-independent assessment of thickness in three-dimensional images. J. Microsc. 185, 67 75.
15. Ho, S.T. Hutmacher, D.W. (2006) A comparison of micro CT with other techniques used in the characterization of scaffolds. Biomaterials 27, 1362 1376.
16. Kak, A.C. Slaney, M. (1998) Principles of Computerized Tomographic Imaging. IEEE Press
17. Kofron, M.D., Li, X. Laurencin, C.T. (2004) Protein-and gene-based tissue engineering in bone repair. Curr. Opin. Biotechnol. 15, 399-405.
18. Komlev, V.S., Peyrin, F., Mastrogiacomo, M., Cedola, A., Papadimitropoulos, A., Rustichelli, F. Cancedda, R. (2006) Kinetics of in vivo bone deposition by bone marrow stromal cells into porous calcium phosphate scaffolds: an X-ray computed microtomography study. Tissue Eng. 12, 3449-3454.
19. Labiche, J.-C., Maton, O., Pascarelli, S., et al 2007) The FReLoN camera as a versatile X-ray detector for time resolved dispersive exafs and diffraction studies of dynamic problems in materials science, chemistry, and catalysis. Rev. Sci. Instrum. 78, 091-301.
20. Langer, M., Cloetens, P. Peyrin, F. (in press) Regularization of phase retrieval with phase-attenuation duality prior for 3D holotomography. IEEE Trans. Im. Proc.
21. Langstaff, S., Sayer, M., Smith, T. J.N. Pugh, S.M. (2001) Resorbable bioceramics based on stabilized calcium phosphates. part ii: evaluation of biological response. Biomaterials 22, 135-150.
22. Lin, A. S.P., Barrows, T.H., Cartmell, S.H. Guldberg, R.E. (2003) Microarchitectural and mechanical characterization of oriented porous polymer scaffolds. Biomaterials 24, 481-489.
23. Mastrogiacomo, M., Corsi, A., Francioso, E., et al 2006) Reconstruction of extensive long bone defects in sheep using resorbable bioceramics based on silicon stabilized tricalcium phosphate. Tissue Eng. 12, 1261-1273.
24. Mastrogiacomo, M., Papadimitropoulos, A., Cedola, A., et al 2007) Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: evidence for a coupling between bone formation and scaffold resorption. Biomaterials 28, 1376-1384.
25. Mayo, S., Davis, T., Gureyev, T., et al 2003) X-ray phase-contrast microscopy and microtomography. Opt. Express 11, 2289-2302.
26. Momose, A. Fukuda, J. (1995) Phase-contrast radiographs of nonstained rat cerebellar specimen. Med. Phys. 22, 375-379.
27. Nugent, K., Gureyev, T., Cookson, D., Paganin, D. Barnea, Z. (1996) Quantitative phase imaging using hard X rays. Phys. Rev. Lett. 77, 2961-2964. (Pubitemid 126624182)
28. Otsu, N. (1979) A threshold selection method from gray-level histograms. IEEE Trans. Sys. Man. Cyber. 9, 62-66.
29. Paganin, D., Mayo, S.C., Gureyev, T.E., Miller, P.R. Wilkins, S.W. (2002) Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object. J. Microsc. 206, 33-40.
30. Papadimitropoulos, A., Mastrogiacomo, M., Peyrin, F., Molinari, E., Komlev, V.S., Rustichelli, F. Cancedda, R. (2007) Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: an X-ray computed microtomography study. Biotechnol. Bioeng. 98, 271-281.
31. Reid, J.W., Pietak, A., Sayer, M., Dunfield, D. Smith, T. (2005) Phase formation and evolution in the silicon substituted tricalcium phosphate/apatite system. Biomaterials 26, 2887-2897.
32. Salomé, M., Peyrin, F., Cloetens, P., Odet, C., Laval-Jeantet, A.-M., Baruchel, J. Spanne, P. (1999) A synchrotron radiation microtomography system for the analysis of trabecular bone samples. Med. Phys. 26, 2194-2204.
33. Sanchez del Rio, M. Dejus, R.J. (2004) Xop 2.1: a new version of the X-ray optics software toolkit. Proc. Synchrotron Radiation Instrumentation: Eighth International Conference (ed. by T. Warwick et al. pp. 784-787. Americal Institute of Physics
34. Sayer, M., Stratilatov, A.D., Reid, J., et al 2003) Structure and composition of silicon-stabilized tricalcium phosphate. Biomaterials 24, 369-382.
35. Snigirev, A., Snigireva, I., Kohn, V., Kuznetsov, S. Schelokov, I. (1995) On the possibilities of X-ray phase contrast microimaging by coherent high-energy synchrotron radiation. Rev. Sci. Instrum. 66, 54-86.
36. Thomsen, J.S., Laib, A., Koller, B., Prohaska, S., Mosekilde, L. Gowin, W. (2005) Stereological measures of trabecular bone structure: comparison of 3D micro computed tomography with 2D histological sections in human proximal tibial bone biopsies. J. Microsc. 218, 171-179.
37. Tikhonov, A.N. Arsenin, V.A. (1977) Solution of Ill-posed Problems. Winston
38. Tortelli, F., Pujic, N., Liu, Y., Laroche, N., Vico, L. Cancedda, R. (2009) Osteoblast and osteoclast differentiation in an in vitro three-dimensional model of bone. Tissue Eng. 15, 2373-2383.
39. Wilkins, S.W., Gureyev, T.E., Gao, D., Pogany, A. Stevenson, A.W. (1996) Phase-contrast imaging using polychromatic hard X-rays. Nature 384, 335-338.