Intestinal absorption of fibrinolytic and proteolytic lumbrokinase extracted from earthworm, Eisenia andrei

Korean Journal of Physiology and Pharmacology

Volumn 14, Issue 2, 2010, Pages 71-75

  Yan, Xiang Mei ^a   Kim, Chung Hyo ^a   Lee, Chul Kyu ^b   Shin, Jang Sik ^b   Cho, Il Hwan ^b   Sohn, Uy Dong ^a  

^a Chung Ang University   (South Korea)

^b Shinpoong Pharmaceutical Co   (South Korea)

Author keywords

Eisenia andrei; Fibrinolytic enzymes; Gut sac; Intestinal proteinase absorption; Recirculation intestinal perfusion

Indexed keywords

ISOENZYME; LUMBROKINASE; POLYCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD; DOSE RESPONSE; DRUG ABSORPTION; EARTHWORM; EISENIA ANDREI; FIBRINOLYSIS; IMMUNOLOGY; INTESTINE; INTESTINE ABSORPTION; INTESTINE EPITHELIUM; MALE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PROTEIN DEGRADATION; RAT; SEROSA; TIME; WESTERN BLOTTING;

EID: 77952593904     PISSN: 12264512     EISSN: None     Source Type: Journal    
DOI: 10.4196/kjpp.2010.14.2.71     Document Type: Article

References (17)

1. Mihara H, Sumi H, Yoneta T, Mizumoto H, Ikeda R, Seiki M, Maruyama M. A novel fibrinolytic enzyme extracted from the earthworm, lumbricus rubellus. Jpn J Physiol. 1991;41:461-472.
2. Cho IH, Choi ES, Lim HG, Lee HH. Purification and characterization of six fibrinolytic serine-proteases from earthworm lumbricus rubellus. J Biochem Mol Biol. 2004;37:199-205.
3. Castell JV, Friedrich G, Kuhn CS, Poppe GE. Intestinal absorption of undegraded proteins in men: Presence of bromelain in plasma after oral intake. Am J Physiol. 1997;273:G139-146. (Pubitemid 27366382)
4. Fan Q, Wu C, Li L, Fan R, Hou Q, He R. Some features of intestinal absorption of intact fibrinolytic enzyme iii-1 from lumbricus rubellus. Biochim Biophys Acta. 2001;1526:286-292. (Pubitemid 32532133)
5. Fujita M, Hong K, Ito Y, Misawa S, Takeuchi N, Kariya K, Nishimuro S. Transport of nattokinase across the rat intestinal tract. Biol Pharm Bull. 1995;18:1194-1196.
6. Vilhardt H, Lundin S. In vitro intestinal transport of vasopressin and its analogues. Acta Physiol Scand. 1986;126:601-607.
7. Lee CK, Shin JS, Kim BS, Cho IH, Kim YS, Lee EB. Antith-rombotic effects by oral administration of novel proteinase fraction from earthworm eisenia andrei on venous thrombosis model in rats. Arch Pharm Res. 2007;30:475-480.
8. Choi NS, Kim BY, Lee JY, Yoon KS, Han KY, Kim SH. Relationship between acrylamide concentration and enzymatic activity in an improve single fibrin zymogram gel system. J Biochem Mol Biol. 2002;35:236-238. (Pubitemid 34389494)
9. Zhou J, Fan R, Wu C, He RQ. Assay of lumbrokinase with a chromophoric substrate. Protein Pept Lett. 1997;4:409-414.
10. Kim SH, Choi NS, Lee WY. Fibrin zymography: a direct analysis of fibrinolytic enzymes on gels. Anal Biochem. 1998;263:115-116.
11. Schanker LS, Tocco DJ, Brodie BB, Hogben CA. Absorption of drugs from the rat small intestine. J Pharmacol Exp Ther. 1958;123:81-88.
12. Mortz E, Krogh TN, Vorum H, Gorg A. Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis. Proteomics. 2001;1:1359-1363.
13. Landry F, Lombardo CR, Smith JW. A method for application of samples to matrix-assisted laser desorption ionization time-3 of-flight targets that enhances peptide detection. Anal Biochem. 2000;279:1-8. (Pubitemid 30148460)
14. Shevchenko A, Jensen ON, Podtelejnikov AV, Sagliocco F, Wilm M, Vorm O, Mortensen P, Boucherie H, Mann M. Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels. Proc Natl Acad Sci USA 1996;93:14440-14445. (Pubitemid 26418985)
15. Nakajima N, Ishihara K, Sugimoto M, Sumi H, Mikuni K, Hamada H. Chemical modification of earthworm fibrinolytic enzyme with human serum albumin fragment and characterization of the protease as a therapeutic enzyme. Biosci Biotechnol Biochem. 1996;60:293-300. (Pubitemid 3042641)
16. Gardner ML. Gastrointestinal absorption of intact proteins. Annu Rev Nutr. 1988;8:329-350.
17. Sanderson IR, Walker WA. Uptake and transport of macro-molecules by the intestine: Possible role in clinical disorders (an update). Gastroenterology. 1993;104:622-639.