Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: A way for producing functional protein in prokaryotic microorganism

Journal of Biotechnology

Volumn 147, Issue 2, 2010, Pages 130-135

  Ren, Xiaofeng ^a   Wang, Mingcui ^a   Yin, Jiechao ^a   Ren, Yudong ^a   Li, Guangxing ^a  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

Author keywords

GP5 protein; PCR; Prokaryotic expression; PRRSV

Indexed keywords

BIOINFORMATICS ANALYSIS; FUNCTIONAL PROTEINS; FUSED GENES; GENE FRAGMENTS; HETEROLOGOUS EXPRESSION; IMMUNOREACTIVITIES; PCR; PROKARYOTIC EXPRESSION; PROKARYOTIC MICROORGANISM; PURIFIED PROTEIN; SIGNAL PEPTIDE; TRANSMEMBRANE SEQUENCES; WESTERN BLOTS;

ANTIBODIES; BIOINFORMATICS; ENCODING (SYMBOLS); GENE ENCODING; GLYCOPROTEINS; POLYMERASE CHAIN REACTION; VIRUSES;

GENE EXPRESSION;

ANTIGEN; GLYCOPROTEIN V; ISOPROPYL THIOGALACTOSIDE; POLYCLONAL ANTIBODY; PROTEIN ANTIBODY; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; VIRUS GLYCOPROTEIN;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTERIVIRUS; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE FUSION; GENE SEQUENCE; HETEROLOGOUS EXPRESSION; IMMUNOREACTIVITY; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; VIRUS DETECTION; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, VIRAL; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; IMMUNOBLOTTING; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; RABBITS; RECOMBINANT FUSION PROTEINS; VIRAL ENVELOPE PROTEINS;

ORYCTOLAGUS CUNICULUS; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; PROKARYOTA;

EID: 77952580827     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.03.013     Document Type: Article

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