ScVEGF-PEG-HBED-CC and scVEGF-PEG-NOTA conjugates: comparison of easy-to-label recombinant proteins for [68Ga]PET imaging of VEGF receptors in angiogenic vasculature

Nuclear Medicine and Biology

Volumn 37, Issue 4, 2010, Pages 405-412

  Eder, Matthias ^a   Krivoshein, Arcadius V ^b   Backer, Marina ^b   Backer, Joseph M ^b   Haberkorn, Uwe ^c   Eisenhut, Michael ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b SIBTECH INC   (United States)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

68Ga; HBED CC; NOTA; PET imaging; VEGFR 2

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; CARBOXYL GROUP; MALEIMIDE; N HYDROXYSUCCINIMIDE; PEGYLATED SINGLE CHAIN VASCULOTROPIN 1,4,7 TRIAZACYCLONONANE 1,4,7 TRIACETIC ACID GA 68; PEGYLATED SINGLE CHAIN VASCULOTROPIN N,N' BIS [2 HYDROXY 5 (CARBOXYETHYL)BENZYL]ETHYLENEDIAMINE N,N' DIACETIC ACID GA 68; RADIOLIGAND; RECOMBINANT PROTEIN; TRACER; UNCLASSIFIED DRUG; VASCULOTROPIN RECEPTOR; VASCULOTROPIN RECEPTOR 2;

ANGIOGENESIS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL STRAIN HEK293; COMPLEX FORMATION; CONJUGATION; CONTROLLED STUDY; DRUG ACCUMULATION; DRUG DISTRIBUTION; DRUG LIVER LEVEL; DRUG STABILITY; DRUG SYNTHESIS; DRUG TARGETING; DRUG TUMOR LEVEL; FEMALE; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IN VIVO STUDY; ISOTOPE LABELING; MOLECULAR IMAGING; MOUSE; NONHUMAN; POSITRON EMISSION TOMOGRAPHY; PROTEIN EXPRESSION; RADIOCHEMISTRY; ROOM TEMPERATURE; TUMOR XENOGRAFT; VASCULARIZATION;

ACETIC ACIDS; ANIMALS; CELL LINE, TUMOR; CHELATING AGENTS; CYSTEINE; ETHYLENEDIAMINES; GALLIUM RADIOISOTOPES; HETEROCYCLIC COMPOUNDS; HUMANS; ISOTOPE LABELING; MICE; NEOVASCULARIZATION, PATHOLOGIC; POLYETHYLENE GLYCOLS; POSITRON-EMISSION TOMOGRAPHY; RECEPTORS, VASCULAR ENDOTHELIAL GROWTH FACTOR; RECOMBINANT PROTEINS; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 77952543436     PISSN: 09698051     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nucmedbio.2010.02.001     Document Type: Article

References (34)

1. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med 1995, 1:27-31.
2. Ferrara N. Vascular endothelial growth factor: basic science and clinical progress. Endocr Rev 2004, 25:581-611.
3. Ferrara N., Gerber H.P., LeCouter J. The biology of VEGF and its receptors. Nat Med 2003, 9:669-676.
4. Manley P.W., Bold G., Bruggen J., Fendrich G., Furet P., Mestan J., et al. Advances in the structural biology, design and clinical development of VEGF-R kinase inhibitors for the treatment of angiogenesis. Biochim Biophys Acta 2004, 1697:17-27.
5. Miller J.C., Pien H.H., Sahani D., Sorensen A.G., Thrall J.H. Imaging angiogenesis: applications and potential for drug development. J Natl Cancer Inst 2005, 97:172-187.
6. Haubner R., Wester H.J. Radiolabeled tracers for imaging of tumor angiogenesis and evaluation of anti-angiogenic therapies. Curr Pharm Des 2004, 10:1439-1455.
7. Cai W., Chen X. Multimodality molecular imaging of tumor angiogenesis. J Nucl Med 2008, 49(Suppl 2):113S-128S.
8. Cai W., Chen K., Mohamedali K.A., Cao Q., Gambhir S.S., Rosenblum M.G., et al. PET of vascular endothelial growth factor receptor expression. J Nucl Med 2006, 47:2048-2056.
9. Blankenberg F.G., Levashova L., Sarkar S.K., Pizzonia J., Backer M.V., Backer J.M. Noninvasive assessment of tumor VEGF receptors in response to treatment with pazopanib: a molecular imaging study. Transl Oncol 2010, 3:56-64.
10. 123I-VEGF165). Biodistribution, safety and radiation dosimetry in patients with pancreatic carcinoma. Q J Nucl Med Mol Imaging 2004, 48:198-206.
11. Li S., Peck-Radosavljevic M., Koller E., Koller F., Kaserer K., Kreil A., et al. Characterization of (123)I-vascular endothelial growth factor-binding sites expressed on human tumour cells: possible implication for tumour scintigraphy. Int J Cancer 2001, 91:789-796.
12. Li S., Peck-Radosavljevic M., Kienast O., Preitfellner J., Hamilton G., Kurtaran A., et al. Imaging gastrointestinal tumours using vascular endothelial growth factor-165 (VEGF165) receptor scintigraphy. Ann Oncol 2003, 14:1274-1277.
13. Lu E., Wagner W.R., Schellenberger U., Abraham J.A., Klibanov A.L., Woulfe S.R., et al. Targeted in vivo labeling of receptors for vascular endothelial growth factor: approach to identification of ischemic tissue. Circulation 2003, 108:97-103.
14. Chan C., Sandhu J., Guha A., Scollard D.A., Wang J., Chen P., et al. A human transferrin-vascular endothelial growth factor (hnTf-VEGF) fusion protein containing an integrated binding site for (111)In for imaging tumor angiogenesis. J Nucl Med 2005, 46:1745-1752.
15. Blankenberg F.G., Mandl S., Cao Y.A., O'Connell-Rodwell C., Contag C., Mari C., et al. Tumor imaging using a standardized radiolabeled adapter protein docked to vascular endothelial growth factor. J Nucl Med 2004, 45:1373-1380.
16. Backer M.V., Gaynutdinov T.I., Patel V., Bandyopadhyaya A.K., Thirumamagal B.T., Tjarks W., et al. Vascular endothelial growth factor selectively targets boronated dendrimers to tumor vasculature. Mol Cancer Ther 2005, 4:1423-1429.
17. Blankenberg F.G., Backer M.V., Levashova Z., Patel V., Backer J.M. In vivo tumor angiogenesis imaging with site-specific labeled (99m)Tc-HYNIC-VEGF. Eur J Nucl Med Mol Imaging 2006, 33:841-848.
18. Backer M.V., Levashova Z., Levenson R., Blankenberg F.G., Backer J.M. Cysteine-containing fusion tag for site-specific conjugation of therapeutic and imaging agents to targeting proteins. Methods Mol Biol 2008, 494:275-294.
19. Backer M.V., Patel V., Jehning B.T., Claffey K.P., Backer J.M. Surface immobilization of active vascular endothelial growth factor via a cysteine-containing tag. Biomaterials 2006, 27:5452-5458.
20. Backer M.V., Levashova Z., Patel V., Jehning B.T., Claffey K., Blankenberg F.G., et al. Molecular imaging of VEGF receptors in angiogenic vasculature with single-chain VEGF-based probes. Nat Med 2007, 13:504-509.
21. Lundqvist H., Tolmachev V. Targeting peptides and positron emission tomography. Biopolymers 2002, 66:381-392.
22. Eder M., Wangler B., Knackmuss S., Legall F., Little M., Haberkorn U., et al. Tetrafluorophenolate of HBED-CC: a versatile conjugation agent for (68)Ga-labeled small recombinant antibodies. Eur J Nucl Med Mol Imaging 2008, 35:1878-1886.
23. 68Ga-based PET-radiopharmaceuticals at room temperature. Bioconjug Chem 2008, 19:569-573.
24. Maecke H.R., Hofmann M., Haberkorn U. (68)Ga-labeled peptides in tumor imaging. J Nucl Med 2005, 46(Suppl 1):172S-178S.
25. Schuhmacher J., Maier-Borst W. A new Ge-68/Ga-68 radioisotope generator system for production of Ga-68 in dilute HCl. Int J Appl Radiat Isot 1981, 32:31-36.
26. Backer M.V., Backer J.M. Functionally active VEGF fusion proteins. Protein Expr Purif 2001, 23:1-7.
27. Levashova Z., Backer M., Backer J.M., Blankenberg F.G. Direct site-specific labeling of the Cys-tag moiety in scVEGF with technetium 99m. Bioconjug Chem 2008, 19:1049-1054.
28. Collingridge D.R., Carroll V.A., Glaser M., Aboagye E.O., Osman S., Hutchinson O.C., et al. The development of [(124)I]iodinated-VG76e: a novel tracer for imaging vascular endothelial growth factor in vivo using positron emission tomography. Cancer Res 2002, 62:5912-5919.
29. Nagengast W.B., de Vries E.G., Hospers G.A., Mulder N.H., de Jong J.R., Hollema H., et al. In vivo VEGF imaging with radiolabeled bevacizumab in a human ovarian tumor xenograft. J Nucl Med 2007, 48:1313-1319.
30. Wang H., Cai W., Chen K., Li Z.B., Kashefi A., He L., et al. A new PET tracer specific for vascular endothelial growth factor receptor 2. Eur J Nucl Med Mol Imaging 2007, 34:2001-2010.
31. 68Ga-PET: a powerful generator-based alternative to cyclotron-based PET radiopharmaceuticals. Contrast Media Mol Imaging 2008, 3:67-77.
32. Velikyan I., Beyer G.J., Langstrom B. Microwave-supported preparation of (68)Ga bioconjugates with high specific radioactivity. Bioconjug Chem 2004, 15:554-560.
33. Behr T.M., Goldenberg D.M., Becker W. Reducing the renal uptake of radiolabeled antibody fragments and peptides for diagnosis and therapy: present status, future prospects and limitations. Eur J Nucl Med 1998, 25:201-212.
34. Saban M.R., Backer J.M., Backer M.V., Maier J., Fowler B., Davis C.A., et al. VEGF receptors and neuropilins are expressed in the urothelial and neuronal cells in normal mouse urinary bladder and are upregulated in inflammation. Am J Physiol Renal Physiol 2008, 295:F60-F72.