Use of a novel assay to evaluate enzymes that produce hydrogen sulfide in Fusobacterium nucleatum

Journal of Microbiological Methods

Volumn 80, Issue 3, 2010, Pages 313-315

  Yoshida, Yasuo ^a   Ito, Shuntaro ^a   Tamura, Haruki ^a   Kunimatsu, Kazushi ^a  

^a IWATE MEDICAL UNIVERSITY   (Japan)

Author keywords

Active staining; Fusobacterium nucleatum; Hydrogen sulfide; Polyacrylamide gel electrophoresis with renaturation

Indexed keywords

HYDROGEN SULFIDE;

ARTICLE; ENZYME ASSAY; ENZYME RENATURATION; FUSOBACTERIUM; FUSOBACTERIUM ANIMALIS; FUSOBACTERIUM NUCLEATUM; FUSOBACTERIUM POLYMORPHUM; FUSOBACTERIUM VINCENTII; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; STAINING; CHEMISTRY; ENZYMOLOGY; FUSOBACTERIUM INFECTION; HUMAN; METABOLISM; METHODOLOGY; MICROBIOLOGY; PERIODONTAL DISEASE; SPECIES DIFFERENCE;

FUSOBACTERIUM NUCLEATUM;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; FUSOBACTERIUM INFECTIONS; FUSOBACTERIUM NUCLEATUM; HUMANS; HYDROGEN SULFIDE; MOLECULAR WEIGHT; PERIODONTAL DISEASES; SPECIES SPECIFICITY; STAINING AND LABELING;

EID: 77952297668     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.01.015     Document Type: Article

References (17)

1. Awano N., Wada M., Mori H., Nakamori S., Takagi H. Identification and functional analysis of Escherichia coli cysteine desulfhydrases. Appl. Environ. Microbiol. 2005, 71:4149-4152.
2. Barrett J.F., Curtiss R. Renaturation of dextranase activity from culture supernatant fluids of Streptococcus sobrinus after sodium dodecylsulfate polyacrylamide gel electrophoresis. Anal. Biochem. 1986, 158:365-370.
3. Beauchamp R.O., Bus J.S., Popp J.A., Boreiko C.J., Andjelkovich D.A. A critical review of the literature on hydrogen sulfide toxicity. Crit. Rev. Toxicol. 1984, 13:25-97.
4. Claesson R., Edlund M.B., Persson S., Carlsson J. Production of volatile sulfur compounds by various Fusobacterium species. Oral Microbiol. Immunol. 1990, 5:137-142.
5. Dzink J.L., Sheenan M.T., Socransky S.S. Proposal of three subspecies of Fusobacterium nucleatum Knorr 1922: Fusobacterium nucleatum subsp. nucleatum subsp. nov., comb. nov.; Fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb. nov.; and Fusobacterium nucleatum subsp. vincentii subsp. nov., nom. rev., comb. nov. Int. J. Syst. Bacteriol. 1990, 40:74-78.
6. Gharbia S.E., Shah H.N. Fusobacterium nucleatum subsp. fusiforme subsp. nov. and Fusobacterium nucleatum subsp. animalis subsp. nov. as additional subspecies within Fusobacterium nucleatum. Int. J. Syst. Bacteriol. 1992, 42:296-298.
7. Gharbia S.E., Shah H.N., Lawson P.A., Haapasalo M. Distribution and frequency of Fusobacterium nucleatum subspecies in the human oral cavity. Oral Microbiol. Immunol. 1990, 5:324-327.
8. Ito S., Nagamune H., Tamura H., Yoshida Y. Identification and molecular analysis of βC-S lyase producing hydrogen sulfide in Streptococcus intermedius. J. Med. Microbiol. 2008, 57:1411-1419.
9. Kezuka Y., Yoshida Y., Nonaka T. Crystallization and preliminary X-ray analysis of βC-S lyases from two oral streptococci. Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 2009, 65:874-877.
10. Persson S., Edlund M.B., Claesson R., Carlsson J. The formation of hydrogen sulfide and methyl mercaptan by oral bacteria. Oral Microbiol. Immunol. 1990, 5:195-201.
11. Pianotti R., Lachette S., Dills S. Desulfuration of cysteine and methionine by Fusobacterium nucleatum. J. Dent. Res. 1986, 65:913-917.
12. Schmidt A. D-Cysteine desulfhydrase from spinach. Methods Enzymol. 1987, vol. 143:449-451. Academic Press, Inc, New York. W. Jakoby, O. Griffith (Eds.).
13. Yaegaki K., Qian W., Murata T., Imai T., Sato T., Tanaka T., Kamoda T. Oral malodorous compound causes apoptosis and genomic DNA damage in human gingival fibroblasts. J. Periodontal. Res. 2008, 43:391-399.
14. Yang G., Sun X., Wang R. Hydrogen sulfide-induced apoptosis of human aorta smooth muscle cells via the activation of mitogen-activated protein kinases and caspase-3. Faseb J. 2004, 18:1782-1784.
15. Yoshida Y., Nakano Y., Amano A., Yoshimura M., Fukamachi H., Oho T., Koga T. lcd from Streptoccus anginosus encodes a C-S lyase with α, β-elimination activity that degrades L-cysteine. Microbiology 2002, 148:3961-3970.
16. Yoshida Y., Negishi M., Amano A., Oho T., Nakano Y. Differences in the βC-S lyase activities of viridans group streptococci. Biochem. Biophys. Res. Commun. 2003, 300:55-60.
17. Yoshida Y., Sasaki T., Ito S., Tamura H., Kunimatsu K., Kato H. Identification and molecular characterization of tryptophanase encoded by tnaA in Porphyromonas gingivalis. Microbiology 2009, 155:968-978.