Development of a conditional gene expression system using a zearalenone-inducible promoter for the ascomycete fungus gibberella zeae

Applied and Environmental Microbiology

Volumn 76, Issue 10, 2010, Pages 3089-3096

  Lee, Jungkwan ^a   Son, Hokyoung ^a   Lee, Seunghoon ^a   Park, Ae Ran ^a   Lee, Yin Won ^a  

^a Seoul National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MECHANISMS; CONCENTRATION-DEPENDENT; CONDITIONAL EXPRESSIONS; ESSENTIAL GENE; FUSARIUM HEAD BLIGHTS; GENE DELETION; GENE FUSION; GENE OVER-EXPRESSION; GFP EXPRESSION; GIBBERELLA ZEAE; GREEN FLUORESCENT PROTEIN; INDUCIBLE PROMOTER; METHIONINE BIOSYNTHESIS; MICROARRAY ANALYSIS; MOLECULAR METHODS; NORTHERN BLOT ANALYSIS; PLANT PATHOGEN; SEXUAL REPRODUCTION; SMALL GRAINS; TOXIN PRODUCTION; ZEARALENONE;

AGRICULTURAL PRODUCTS; BIOCHEMISTRY; BIOSENSORS; FUNGI; POLLUTION DETECTION;

GENE EXPRESSION;

GREEN FLUORESCENT PROTEIN; ZEARALENONE;

ESTROGENIC COMPOUND; FOOD QUALITY; FUNGAL DISEASE; FUNGUS; GENE EXPRESSION; GENETIC ANALYSIS; GROWTH RATE; METABOLITE; PATHOGENICITY; SEXUAL REPRODUCTION; TOXIN;

ARTICLE; DRUG EFFECT; GENE DELETION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; GIBBERELLA; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; PROMOTER REGION; REGULATORY SEQUENCE;

GENE DELETION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, FUNGAL; GIBBERELLA; GREEN FLUORESCENT PROTEINS; PROMOTER REGIONS, GENETIC; REGULATORY SEQUENCES, NUCLEIC ACID; ZEARALENONE;

ASCOMYCOTA; FUNGI; FUSARIUM; GIBBERELLA; GIBBERELLA ZEAE;

EID: 77952276909     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02999-09     Document Type: Article

References (39)

1. Bacon, C. W., J. D. Robbins, and J. K. Porter. 1977. Media for identification of Gibberella zeae and production of F-2-(Zearalenone). Appl. Environ. Microbiol. 33:445-449.
2. Chevanne, D., E. Bastiaans, A. Debets, S. J. Saupe, C. Clave, and M. Paoletti. 2009. Identification of the het-r vegetative incompatibility gene of Podospora anserina as a member of the fast evolving HNWD gene family. Curr. Genet. 55:93-102.
3. Desjardins, A. E. 2006. Fusarium mycotoxins: chemistry, genetics and biology. APS Press, St. Paul, MN.
4. Gaffoor, I., and F. Trail. 2006. Characterization of two polyketide synthase genes involved in zearalenone biosynthesis in Gibberella zeae. Appl. Environ. Microbiol. 72:1793-1799.
5. 5. Gebhart, D., A. K. Bahrami, and A. Sil. 2006. Identification of a copperinducible promoter for use in ectopic expression in the fungal pathogen Histoplasma capsulatum. Eukaryot. Cell 5:935-944. (Pubitemid 43895023)
6. Han, Y.-K., T. Lee, K.-H. Han, S.-H. Yun, and Y.-W. Lee. 2004. Functional analysis of the homoserine O-acetyltransferase gene and its identification as a selectable marker in Gibberella zeae. Curr. Genet. 46:205-212.
7. Harris, S. D. 2005. Morphogenesis in germinating Fusarium graminearum macroconidia. Mycologia 97:880-887.
8. Horwitz, B. A., A. Sharon, S. W. Lu, V. Ritter, T. M. Sandrock, O. C. Yoder, and B. G. Turgeon. 1999. A G protein alpha subunit from Cochliobolus heterostrophus involved in mating and appressorium formation. Fungal Genet. Biol. 26:19-32.
9. Hou, Z., C. Xue, Y. Peng, T. Katan, H. C. Kistler, and J.-R. Xu. 2002. A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection. Mol. Plant Microbe Interact. 15:1119-1127.
10. Jurgenson, J. E., R. L. Bowden, K. A. Zeller, J. F. Leslie, N. J. Alexander, and R. D. Plattner. 2002. A genetic map of Gibberella zeae (Fusarium graminearum). Genetics 160:1451-1460.
11. Kim, J.-E., J. Jin, H. Kim, J.-C. Kim, S.-H. Yun, and Y.-W. Lee. 2006. GIP2, a putative transcription factor that regulates the aurofusarin biosynthetic gene cluster in Gibberella zeae. Appl. Environ. Microbiol. 72:1645-1652.
12. Kim, Y.-T., Y.-R. Lee, J. Jin, K.-H. Han, H. Kim, J.-C. Kim, T. Lee, S.-H. Yun, and Y.-W. Lee. 2005. Two different polyketide synthase genes are required for synthesis of zearalenone in Gibberella zeae. Mol. Microbiol. 58:1102-1113.
13. Lee, J., J. E. Jurgenson, J. F. Leslie, and R. L. Bowden. 2008. Alignment of genetic and physical maps of Gibberella zeae. Appl. Environ. Microbiol. 74:2349-2359.
14. 14. Lee, J., J. F. Leslie, and R. L. Bowden. 2008. Expression and function of sex pheromones and receptors in the homothallic ascomycete Gibberella zeae. Eukaryot. Cell 7:1211-1221. (Pubitemid 351969335)
15. 15. Lee, T., Y.-K. Han, K.-H. Kim, S.-H. Yun, and Y.-W. Lee. 2002. Tri13 and Tri7 determine deoxynivalenol- and nivalenol-producing chemotypes of Gibberella zeae. Appl. Environ. Microbiol. 68:2148-2154. (Pubitemid 34477807)
16. Leslie, J. F. 1993. Fungal vegetative compatibility. Annu. Rev. Phytopathol. 31:127-150.
17. Leslie, J. F., and B. A. Summerell. 2006. The Fusarium laboratory manual. Blackwell Professional, Ames, IA.
18. Lysoe, E., K. R. Bone, and S. S. Klemsdal. 2009. Real-time quantitative expression studies of the zearalenone biosynthetic gene cluster in Fusarium graminearum. Phytopathology 99:176-184.
19. Lysoe, E., S. S. Klemsdal, K. R. Bone, R. J. Frandsen, T. Johansen, U. Thrane, and H. Giese. 2006. The PKS4 gene of Fusarium graminearum is essential for zearalenone production. Appl. Environ. Microbiol. 72:3924-3932.
20. 20. Mach, R. L., and S. Zeilinger. 2003. Regulation of gene expression in industrial fungi: Trichoderma. Appl. Microbiol. Biotechnol. 60:515-522. (Pubitemid 36169525)
21. Micali, C. O., and M. L. Smith. 2006. A nonself recognition gene complex in Neurospora crassa. Genetics 173:1991-2004.
22. Mirocha, C. J., and C. M. Christensen. 1974. Oestrogenic mycotoxins synthesized by Fusarium sp., p. 129-148. In I. F. H. Purchase (ed.), Mycotoxins. Elsevier Scientific Publishing, Amsterdam, Netherlands.
23. Munson, G. P., D. L. Lam, F. W. Outten, and T. V. O'Halloran. 2000. Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12. J. Bacteriol. 182:5864-5871.
24. Osusky, M., G. Zhou, L. Osuska, R. E. Hancock, W. W. Kay, and S. Misra. 2000. Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens. Nat. Biotechnol. 18:1162-1166.
25. Park, J. N., M. J. Sohn, D. B. Oh, O. Kwon, S. K. Rhee, C. G. Hur, S. Y. Lee, G. Gellissen, and H. A. Kang. 2007. Identification of the cadmium-inducible Hansenula polymorpha SEO1 gene promoter by transcriptome analysis and its application to whole-cell heavy-metal detection systems. Appl. Environ. Microbiol. 73:5990-6000.
26. Proctor, R. H., T. M. Hohn, and S. P. McCormick. 1995. Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Mol. Plant Microbe Interact. 8:593-601.
27. Romero, B., G. Turner, I. Olivas, F. Laborda, and J. R. De Lucas. 2003. The Aspergillus nidulans alcA promoter drives tightly regulated conditional gene expression in Aspergillus fumigatus permitting validation of essential genes in this human pathogen. Fungal Genet. Biol. 40:103-114.
28. Sambrook, J. S., and D. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Seong, K., Z. Hou, M. Tracy, H. C. Kistler, and J.-R. Xu. 2005. Random insertional mutagenesis identifies genes associated with virulence in the wheat scab fungus Fusarium graminearum. Phytopathology 95:744-750.
30. Shoji, J.-Y., J.-I. Maruyama, M. Arioka, and K. Kitamoto. 2005. Development of Aspergillus oryzae thiA promoter as a tool for molecular biological studies. FEMS Microbiol. Lett. 244:41-46.
31. Skadsen, R. W., P. Sathish, M. L. Federico, T. Abebe, J. Fu, and H. F. Kaeppler. 2002. Cloning of the promoter for a novel barley gene, Lem1, and its organ-specific promotion of GFP expression in lemma and palea. Plant Mol. Biol. 49:545-555.
32. Tada, S., K. Gomi, K. Kitamoto, K. Takahashi, G. Tamura, and S. Hara. 1991. Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli beta-glucuronidase gene and analysis of its expression in Aspergillus oryzae. Mol. Gen. Genet. 229:301-306.
33. Verma, N., and M. Singh. 2005. Biosensors for heavy metals. Biometals 18:121-129.
34. Waring, R. B., G. S. May, and N. R. Morris. 1989. Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulincoding genes. Gene 79:119-130.
35. Willyerd, K. L., A. M. Kemp, and A. L. Dawe. 2009. Controlled gene expression in the plant pathogen Cryphonectria parasitica by use of a copperresponsive element. Appl. Environ. Microbiol. 75:5417-5420.
36. Yevtushenko, D. P., V. A. Sidorov, R. Romero, W. W. Kay, and S. Misra. 2004. Wound-inducible promoter from poplar is responsive to fungal infection in transgenic potato. Plant Sci. 167:715-724.
37. Yu, H.-Y., J.-A. Seo, J.-E. Kim, K.-H. Han, W.-B. Shim, S.-H. Yun, and Y.-W. Lee. 2008. Functional analyses of heterotrimeric G protein G alpha and G beta subunits in Gibberella zeae. Microbiology 154:392-401.
38. Yu, J.-H., Z. Hamari, K.-H. Han, J.-A. Seo, Y. Reyes-Dominguez, and C. Scazzocchio. 2004. Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet. Biol. 41:973-981.
39. Zuo, J., P. D. Hare, and N. H. Chua. 2006. Applications of chemical-inducible expression systems in functional genomics and biotechnology. Methods Mol. Biol. 323:329-342.