Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P

Virology Journal

Volumn 7, Issue 1, 2010, Pages

  Zhang, Wen Jun ^a,b   Li, Hong Jian ^a   Li, Yue Qin ^a   Zeng, Zhi Feng ^a   Li, Shi Qian ^a   Zhang, Xin ^a   Zou, Yi ^a   Zhou, Tian Hong ^a  

^a JINAN UNIVERSITY   (China)

^b GUANGDONG PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; EXTERNAL GUIDE SEQUENCES; MESSENGER RNA; OLIGONUCLEOTIDE; RIBONUCLEASE P; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CODON; CONTROLLED STUDY; EXTERNAL GUIDE SEQUENCE; GENE EXPRESSION; GENE SEQUENCE; GENE TARGETING; GENETIC TRANSFECTION; HELA CELL LINE; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; IN VITRO STUDY; MALE; NONHUMAN; PREPUCE; PROTEIN EXPRESSION; RNA CLEAVAGE; RNA DEGRADATION; RNA SEQUENCE; SKIN FIBROBLAST; TRANSLATION INITIATION; UL49 GENE; VIROGENESIS; VIRUS GENE; VIRUS INHIBITION; VIRUS PURIFICATION; VIRUS REPLICATION; WESTERN BLOTTING; CELL LINE; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; DOWN REGULATION; DRUG EFFECT; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHYSIOLOGY; SYNTHESIS; VIROLOGY;

CELL LINE; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DOWN-REGULATION; GENE EXPRESSION REGULATION, VIRAL; HUMANS; OLIGONUCLEOTIDES; RIBONUCLEASE P; VIRAL STRUCTURAL PROTEINS; VIRUS REPLICATION;

EID: 77952264214     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-7-100     Document Type: Article

References (29)

1. Mocarski ES, Courcelle CT: Cytomegaloviruses and their replication. In Fields virology Edited by: Knipe DM, Howley PM. Philadelphia PA: Lippincott-William & Wilkins; 2001:2629-2673.
2. Baldanti F, Lurain N, Gerna G: Clinical and biologic aspects of human cytomegalovirus resistance to antiviral drugs. Human Immunology 2004, 65:403-409.
3. Stein CA, Cheng Y: Antisense oligonucleotides as therapeutic agents-is the bullet really magical? Science 1993, 261:1004-1012.
4. Margraf S, Bittoova M, Vogel JU, Kotchekov R, Doerr HW, Cinatl JJ: Antisense oligonucleotide ISIS 2922 targets IE-expression and prevents HCMV-IE-induced suppression of TSP-1 and TSP-2 expression. Nucleotides Nucleic Acids 2001, 20:1425-1428.
5. Zhang G, Raghavan B, Kotur M, Cheatham J, Sedmak D, Cook C, Waldman J, Trgovcich J: Antisense transcription in the human cytomegalovirus transcriptome. J Virol 2007, 81:11267-11281.
6. Forster AC, Altman S: External guide sequences for an RNA enzyme. Science 1990, 249:783-786.
7. Werner M, Rosa E, Nordstrom JL, Goldberg AR, George ST: Short oligonucleotides as external guide sequences for site-specific cleavage of RNA molecules with human RNase P. RNA 1990, 4:847-855.
8. Zhang WJ, Li HJ, Li YQ, He HQ, Tang DS, Zhang X, Zhou TH: Construction of an effective M1GS ribozyme targeting HCMV UL97 mRNA segment in vitro. Yi Chuan Xue Bao 2005, 32:1205-1212.
9. Su YZ, Li HJ, Li YQ, Chen HJ, Tang DS, Zhang X, Jiang H, Zhou TH: In vitro construction of effective M1GS ribozymes targeting HCMV UL54 RNA segments. Acta Biochim Biophys Sin 2005, 37:210-214.
10. Frank DN, Pace NR: Ribonuclease P: unity and diversity in a tRNA processing ribozyme. Annu Rev Biochem 1998, 67:153-180.
11. Yuan Y, Hwang ES, Altman S: Targeted cleavage of mRNA by human RNase P. Proc Natl Acad Sci USA 1992, 89:8006-8010.
12. Guerrier-Takada C, Li Y, Altman S: Artificial regulation of gene expression in Escherichia coli by RNase P. Proc Natl Acad Sci USA 1995, 92:11115-11119.
13. Kawa D, Wang J, Yuan Y, Liu F: Inhibition of viral gene expression by human ribonuclease P. RNA 1998, 4:1397-1406.
14. Plehn-Dujowich D, Altman S: Effective inhibition of influenza virus production in cultured cells by external guide sequences and ribonuclease P. Proc Natl Acad Sci USA 1998, 95:7327-7332.
15. Ma M, Benimetskaya L, Lebedeva I, Dignam J, Takle G, Stein CA: Intracellular mRNA cleavage induced through activation of RNase P by nuclease-resistant external guide sequences. Nat Biotechnol 2000, 18:58-61.
16. Dunn W, Chou C, Li H, Hai R, Patterson D, Stolc V, Zhu H: Functional profiling of a human cytomegalovirus genome. Proc Natl Acad Sci USA 2003, 100:14223-14228.
17. Zhu J, Trang P, Kim K, Zhou T, Deng H, Liu F: Effective inhibition of Rta expression and lytic replication of Kaposi's sarcoma-associated herpesvirus by human RNase P. Proc Natl Acad Sci USA 2004, 101:9073-9078.
18. Yang Y, Li H, Zhou T, Kim K, Liu F: Engineered external guide sequences are highly effective in inducing RNase P for inhibition of gene expression and replication of human cytomegalovirus. Nucleic Acids Res 2006, 34:575-583.
19. Trang P, Lee M, Nepomuceno E, Kim J, Zhu H, Liu F: Effective inhibition of human cytomegalovirus gene expression and replication by a ribozyme derived from the catalytic RNA subunit of RNase P from Escherichia coli. Proc Natl Acad Sci USA 2000, 97:5812-5817.
20. Zeng Z, Li H, Li Y, Cui Y, Zhou Q, Zou Y, Yang G, Zhou T: Effective inhibition of human cytomegalovirus gene expression by DNA-based external guide sequences. Acta Biochim Biophys Sin 2009, 41:389-398.
21. Liu F, Altman S: Requirements for cleavage by a modified RNase P of a small model substrate. Nucleic Acids Res 1996, 24:2690-2096.
22. Yuan Y, Altman S: Selection of guide sequences that direct efficient cleavage of mRNA by human ribonuclease P. Science 1994, 263:1269-1273.
23. Altman S, Kirsebom LA: Ribonuclease P. In The RNA world Edited by: Gesteland RF. New York: Cold Spring Harbor Laboratory Press; 1999:351-380.
24. Gopalan V, Vioque A, Altman S: RNase P: variations and uses. J Biol Chem 2002, 277:6759-6762.
25. Trang P, Kim K, Liu F: Developing RNase P ribozymes for gene-targeting and antiviral therapy. Cell Microbiol 2004, 6:499-508.
26. Verma S, Eckstein F: Modified oligonucleotides: synthesis and strategy for users. Annu Rev Biochem 1998, 67:99-134.
27. Kim K, Liu F: Inhibition of gene expression in human cells using RNase P-derived ribozymes and external guide sequences. Biochim Biophys Acta 2007, 1769:603-612.
28. Li H, Trang P, Kim K, Zhou T, Liu F: Effective inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external guide sequence RNA. RNA 2006, 12:63-72.
29. Yang YH, Li H, Zhou T, Kim K, Liu F: Engineered external guide sequences are highly effective in inducing RNase P for inhibition of gene expression and replication of human cytomegalovirus. Nucleic Acids Res 2006, 34:575-583.