Exploring different strategies to express Dengue virus envelope protein in a plant system

Biotechnology Letters

Volumn 32, Issue 6, 2010, Pages 867-875

  Martínez, Carolina Andrea ^a   Topal, Emel ^b   Giulietti, Ana María ^a   Talou, Julián Rodríguez ^a   Mason, Hugh ^b  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b ARIZONA STATE UNIVERSITY   (United States)

Author keywords

Dengue virus; Envelope protein; Magnifection; Nicotiana benthamiana; Transient expression

Indexed keywords

A PLANTS; CRITICAL DOMAIN; DENGUE VIRUS; DIAGNOSTIC KIT; ENVELOPE GLYCOPROTEINS; ENVELOPE PROTEINS; HEPATITIS B CORE ANTIGENS; NICOTIANA BENTHAMIANA; PLANT SYSTEMS; RECOMBINANT PROTEIN; STRUCTURAL PROTEINS; SUBUNIT VACCINE; TRANSIENT EXPRESSION; TRUNCATED VERSIONS;

ANTIGENS; PLANTS (BOTANY); VIRUSES;

ANTIBODIES;

E GLYCOPROTEIN, DENGUE VIRUS TYPE 2; E-GLYCOPROTEIN, DENGUE VIRUS TYPE 2; HEPATITIS B CORE ANTIGEN; RECOMBINANT PROTEIN; VIRUS ANTIBODY; VIRUS ENVELOPE PROTEIN;

ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; GENE DELETION; GENE EXPRESSION; GENE FUSION; GENETICS; IMMUNOLOGY; METABOLISM; METHODOLOGY; TOBACCO; TRANSGENE;

ANTIBODIES, VIRAL; ARTIFICIAL GENE FUSION; BIOTECHNOLOGY; GENE EXPRESSION; HEPATITIS B CORE ANTIGENS; RECOMBINANT PROTEINS; SEQUENCE DELETION; TOBACCO; TRANSGENES; VIRAL ENVELOPE PROTEINS;

DENGUE VIRUS; NICOTIANA BENTHAMIANA;

EID: 77952239645     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0236-6     Document Type: Article

References (18)

1. AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N (2005) A custom-designed recombinant multiepitope protein as a dengue diagnostic reagent. Protein Expr Purif 41: 136-147.
2. Bisht H, Chugh DA, Raje M, Swaminathan S, Khanna N (2002) Recombinant dengue virus type 2 envelope/hepatitis B surface antigen hybrid protein expressed in Pichia pastoris can function as a bivalent immunogen. J Biotechnol 99: 97-110.
3. Gleba Y, Klimyuk V, Marillonnet S (2005) Magnifection-a new platform for expressing recombinant vaccines in plants. Vaccine 23: 2042-2048.
4. Guirakhoo F, Heinz FX, Kunz C (1989) Epitope model of tick-borne encephalitis virus envelope glycoprotein E: analysis of structural properties, role of carbohydrate side chain and conformational changes occurring at acidic pH. Virology 169: 90-99.
5. Huang Z, Santi L, LePore K, Kilbourne J, Arntzen CJ, Mason HS (2006) Rapid, high-level production of hepatitis B core antigen in plant leaf and its immunogenicity in mice. Vaccine 24: 2506-2513.
6. Kelly EP, Greene JJ, King AD, Innis BL (2000) Purified dengue 2 virus envelope glycoprotein aggregates produced by baculovirus are immunogenic in mice. Vaccine 18: 2549-2559.
7. Kumar GBS, Ganapathi TR, Srinivas L, Bapat VA (2007) Plant molecular farming: host systems, technology and products. In: Verpoorte R et al (eds) Applications of plant metabolic engineering. Springer, Netherlands, pp 45-77.
8. Marillonnet S, Giritch A, Gils M, Kandzia R, Klimyuk V, Gleba Y (2004) In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium. PNAS USA 101(18): 6852-6857.
9. Modis Y, Ogata S, Clements D, Harrison SC (2003) A ligand-binding pocket in the dengue virus envelope glycoprotein. PNAS USA 100: 6986-6991.
10. Qi R, Zhang L, Chi C (2008) Biological characteristics of dengue virus and potential targets for drug design. Acta Biochim Biophys Sin 40(2): 91-101.
11. Saejung W, Puttikhunt C, Prommool T, Sojikul P, Tanaka R, Fujiyama K, Malasit P, Seki T (2006) Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production in Escherichia coli trxB and gor double mutant. J Biosci Bioeng 102(4): 333-339.
12. Saejung W, Fujiyama K, Takasaki T, Ito M, Hori K, Malasit P, Watanabe Y, Kurane I, Seki T (2007) Production of Dengue 2 envelope domain III in plant using TMV-based vector system. Vaccine 25: 6646-6654.
13. Sugrue RJ, Cui T, Xu Q, Fu J, Chan Y (1997a) The production of recombinant dengue virus E protein using Escherichia coli and Pichia pastoris. J Virol Methods 69: 159-169.
14. Sugrue RJ, Fu J, Howe J, Chan Y (1997b) Expression of the dengue virus structural protein in Pichia pastoris leads to the generation of virus-like particles. J Gen Virol 78: 1861-1866.
15. Talarico LB, Damonte EB (2007) Interference in Dengue virus adsorption and uncoating by carrageenans. Virology 363: 473-485.
16. Twyman RM, Stoger E, Schillberg S, Christou P, Fischer R (2003) Molecular farming in plants: host systems and expression technology. Trends Biotechnol 21(12): 570-578.
17. Wengler G, Wengler G, Nowak T, Wahn K (1987) Analysis of the influence of proteolytic cleavage on the structural organisation of the surface of the West Nile flavivirus leads to the isolation of a protease-resistant E protein oligomer from the viral surface. Virology 160: 210-219.
18. Werner S, Marillonet S, Hause G, Klimyuk V, Gleba Y (2006) Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A. Proc Natl Acad Sci (USA) 103(47): 17678-17683.