Immobilization of glucose oxidase on Fe3O4/SiO2 magnetic nanoparticles

Biotechnology Letters

Volumn 32, Issue 6, 2010, Pages 817-821

  Huang, Jun ^a   Zhao, Rong ^a   Wang, Hai ^a   Zhao, Wenqi ^a   Ding, Liyun ^a  

^a WUHAN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Activity; Fe3O4 SiO2 magnetic nanoparticles; Glucose oxidase; Immobilization; Stability

Indexed keywords

3-AMINOPROPYLTRIETHOXYSILANE; ACTIVITY; FE3O4/SIO2 MAGNETIC NANOPARTICLES; FREE ENZYME; GLUTARALDEHYDES; INITIAL ACTIVITY; MAGNETIC NANOPARTICLES;

ALDEHYDES; ENZYMES; GLUCOSE; GLUCOSE OXIDASE; GLUCOSE SENSORS; NANOPARTICLES; SILICON COMPOUNDS;

NANOMAGNETICS;

GLUCOSE OXIDASE; IMMOBILIZED ENZYME; MAGNETITE; NANOPARTICLE; SILICON DIOXIDE;

ARTICLE; BIOTECHNOLOGY; CHEMISTRY; ENZYME STABILITY; METABOLISM; METHODOLOGY; PH; PROTEIN BINDING; TEMPERATURE;

BIOTECHNOLOGY; ENZYME STABILITY; ENZYMES, IMMOBILIZED; FERROSOFERRIC OXIDE; GLUCOSE OXIDASE; HYDROGEN-ION CONCENTRATION; NANOPARTICLES; PROTEIN BINDING; SILICON DIOXIDE; TEMPERATURE;

EID: 77952237681     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0217-9     Document Type: Article

References (14)

1. Ahmad A, Akhtar MD, Bhakuni V (2000) Monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme. Biochemistry 40: 1945-1955.
2. Aken BV, Ledent P, Naveau H, Agathos SN (2000) Co-immobilization of manganese peroxidase from Phlebia radiata and glucose oxidase from Aspergillus niger on porous silica beads. Biotechnol Lett 22: 641-646.
3. Cao SS, Liu BL (2009) The preparation and enzyme immobilization of hydrophobic polysiloxane supports. Macromol Biosci 9: 361-368.
4. Caseli L, dos Santos DS Jr, Aroca RF, Oliveira ON Jr (2009) Controlled fabrication of gold nanoparticles biomediated by glucose oxidase immobilized on chitosan layer-by-layer films. Mater Sci Eng C 29: 1687-1690.
5. Hou XH, Liu BL, Deng XB, Zhang BT, Chen HL, Luo R (2007) Covalent immobilization of glucose oxidase onto poly (styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization. Anal Biochem 368: 100-110.
6. Huang CP, Liu SW, Chen TM, Li YK (2008) A new approach for quantitative determination of glucose by using CdSe/ZnS quantum dots. Sens Actuators B 130: 338-342.
7. Kros A, Gerritsen M, Sprakel V, Sommerdijk N, Jansen J, Nolte R (2001) Silica based hybrid material as biocompatible coatings for glucose sensor. Sens Actuators B 81: 68-75.
8. Li QW, Luo GA, Wang YM, Zhang XR (2000) Immobilization of glucose oxidase in sol-gel matrix and its application to fabricate chemiluminescent glucose sensor. Mater Sci Eng C 11: 67-70.
9. Massart T (1981) Preparation of aqueous magnetic liquids in alkaline and acidic media. IEEE Trans Magn 17: 1247-1248.
10. Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzym Microb Technol 40: 1451-1463.
11. 2 magnetic nanoparticles as building blocks for construction of reagentless enzyme-based biosensors. Electrochem Commun 9: 2734-2738.
12. Rauf S, Ihsan A, Akhtar K, Ghauri MA, Rahman M, Anwar MA, Khalid AM (2006) Glucose oxidase immobilization on a novel cellulose acetate-polymethylmethacrylate membrane. J Biotechnol 121: 351-360.
13. Stöber W, Fink A, Bohn E (1968) Controlled growth of mono-disperse silica spheres in the micron size range. J Colloid Interface Sci 26: 62-69.
14. 2 particles enhanced glucose biosensor. Talanta 68: 569-574.