Thiol-based, site-specific and covalent immobilization of biomolecules for single-molecule experiments

Nature Protocols

Volumn 5, Issue 6, 2010, Pages 975-985

  Zimmermann, Julia L ^a,b   Nicolaus, Thomas ^a   Neuert, Gregor ^a,c   Blank, Kerstin ^a,d  

^a UNIVERSITY OF MUNICH   (Germany)

^b MAX PLANCK INSTITUT FÜR EXTRATERRESTRISCHE PHYSIK   (Germany)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; GLYCINE; LIPASE B; MACROGOL; MALEIMIDE; N HYDROXYSUCCINIMIDE; THIOL; IMMOBILIZED ENZYME; IMMOBILIZED PROTEIN; MACROGOL DERIVATIVE; MALEIMIDE DERIVATIVE; N-HYDROXYSUCCINIMIDE; NUCLEIC ACID; SUCCINIMIDE DERIVATIVE; THIOL DERIVATIVE;

AMINO ACID SEQUENCE; ARTICLE; ATOMIC FORCE MICROSCOPY; CANDIDA ANTARCTICA; CARBOXY TERMINAL SEQUENCE; COVALENT BOND; ENZYME IMMOBILIZATION; FLUORESCENCE; HYDROGEN BOND; HYDROLYSIS; PRIORITY JOURNAL; SURFACE PROPERTY; BINDING SITE; CHEMISTRY; PROCEDURES;

BINDING SITES; ENZYMES, IMMOBILIZED; FLUORESCENCE; IMMOBILIZED PROTEINS; MALEIMIDES; MICROSCOPY, ATOMIC FORCE; NUCLEIC ACIDS; POLYETHYLENE GLYCOLS; SUCCINIMIDES; SULFHYDRYL COMPOUNDS;

EID: 77952176659     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.49     Document Type: Article

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