Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 16, 2010, Pages 7377-7382

  Shtutman, Michael ^a   Maliyekkel, Anil ^a,b   Shao, Yu ^c   Carmack, C Steven ^c   Baig, Mirza ^a   Warholic, Natalie ^a   Cole, Kelly ^a   Broude, Eugenia V ^a   Harkins, Timothy T ^d   Ding, Ye ^c   Roninson, Igor B ^a  

^a ORDWAY RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^c WADSWORTH CENTER   (United States)

^d 454 Life Sciences ^*  (United States)

Author keywords

Cancer targets; Functional genomics; Lentiviral vectors; RNA interference; siRNA design

Indexed keywords

COMPLEMENTARY DNA; FIREFLY LUCIFERASE; LENTIVIRUS VECTOR; SHORT HAIRPIN RNA;

APOPTOSIS; ARTICLE; BREAST CARCINOMA; CANCER INHIBITION; CELL CYCLE PROGRESSION; CLONE; GENE IDENTIFICATION; GENE LIBRARY; GENE SEQUENCE; GENE TARGETING; HUMAN; HUMAN CELL; ONCOGENE; PRIORITY JOURNAL; RNA INTERFERENCE; VALIDATION PROCESS;

BREAST NEOPLASMS; CARCINOMA; CELL LINE, TUMOR; DNA; DNA, COMPLEMENTARY; FEMALE; GENE LIBRARY; GENETIC ENGINEERING; GENETIC TECHNIQUES; HUMANS; LENTIVIRUS; MODELS, GENETIC; RNA, SMALL INTERFERING; SEQUENCE ANALYSIS, DNA;

EID: 77952117231     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1003055107     Document Type: Article

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