Use of a label-free quantitative platform based on MS/MS average TIC to calculate dynamics of protein complexes in insulin signaling

Journal of Biomolecular Techniques

Volumn 20, Issue 5, 2009, Pages 272-277

  Yang, Xuemei ^a   Friedman, Adam ^b   Nagpal, Shailender ^c   Perrimon, Norbert ^b   Asara, John M ^a,b  

^a BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c Innovagene Informatics   (United States)

Author keywords

Average TIC; LC MS MS; Networks; Protein protein interaction; Proteomics; Quantification; Signal transduction; Spectral counting

Indexed keywords

INSULIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN; PROTEIN TAG;

AFFINITY LABELING; ANIMAL CELL; ARTICLE; BIOINFORMATICS; COMPLEX FORMATION; COMPUTER PROGRAM; DROSOPHILA; IMMUNOPRECIPITATION; ION CURRENT; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEOMICS; QUANTITATIVE ANALYSIS; SIGNAL TRANSDUCTION; TANDEM MASS SPECTROMETRY; TOTAL ION CURRENT;

ANIMALS; BIOCHEMISTRY; COMPUTATIONAL BIOLOGY; DROSOPHILA; FOURIER ANALYSIS; INSULIN; IONS; MAP KINASE SIGNALING SYSTEM; MODELS, BIOLOGICAL; PROTEIN INTERACTION MAPPING; PROTEOME; PROTEOMICS; SIGNAL TRANSDUCTION; SOFTWARE; TANDEM MASS SPECTROMETRY;

EID: 77952052526     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (3)

1. Gavin AC, Bösche M, Krause R, et al. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 2002;10:141-147.
2. Asara JM, Christofk HR, Freimark LM, Cantley LC. A label-free quantification method by MS/MS TIC compared to SILAC and spectral counting in a proteomics screen. Proteomics 2008;8:994-999.
3. Friedman A, Perrimon N. A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signaling. Nature 2006; 444:230-234.