A five-minute DNA extraction method for expedited detection of Phytophthora ramorum following prescreening using Phytophthora spp. lateral flow devices

Journal of Microbiological Methods

Volumn 81, Issue 2, 2010, Pages 116-120

  Tomlinson, J A ^a   Dickinson, M ^b   Hobden, E ^a   Robinson, S ^a   Giltrap, P M ^a   Boonham, N ^a  

^a FOOD AND ENVIRONMENT RESEARCH AGENCY   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Diagnostic sensitivity; Diagnostic specificity; DNA isolation; Real time PCR; Sudden oak death

Indexed keywords

ACCURACY; ANALYTICAL EQUIPMENT; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CONTROLLED STUDY; DNA EXTRACTION; INTERMETHOD COMPARISON; LATERAL FLOW DEVICE; NONHUMAN; PHYTOPHTHORA; PHYTOPHTHORA RAMORUM; PREDICTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE;

DNA; FILTRATION; MICROBIOLOGICAL TECHNIQUES; PHYTOPHTHORA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

PHYTOPHTHORA; PHYTOPHTHORA RAMORUM;

EID: 77951978475     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.02.006     Document Type: Article

References (16)

1. Anonymous Commission decision (2002/757/EC) of 19 September 2002 on provisional emergency phytosanitary measures to prevent the introduction into and the spread within the Community of Phytophthora ramorum Werres. Off. J. Eur. Communities 2002-09-20 2002, L 252:37-39.
2. Anonymous EPPO diagnostic protocol for regulated pests: Phytophthora ramorum. EPPO Bull. 2006, 36:145-155.
3. Bilodeau G.J., Lévesque C.A., de Cock A.W.A.M., Duchaine C., Brière S., Uribe P., et al. Molecular detection of Phytophthora ramorum by real-time polymerase chain reaction using TaqMan, SYBR Green, and molecular beacons. Phytopathology 2007, 97:632-642.
4. Danks, C., Boonham, N., 2007. Purification method and kits. Patent WO/2007/104962.
5. Hayden K.J., Rizzo D., Tse J., Garbelotto M. Detection and quantification of Phytophthora ramorum from Californian forests using a real-time polymerase chain reaction assay. Phytopathology 2004, 94:1075-1083.
6. Hayden K., Ivors K., Wilkinson C., Garbelotto M. TaqMan chemistry for Phytophthora ramorum detection and quantification, with a comparison of diagnostic methods. Phytopathology 2006, 96:846-854.
7. Hughes K.J.D., Tomlinson J.A., Griffin R.L., Boonham N., Inman A.J., Lane C.R. Development of a one-step real-time PCR assay for diagnosis of Phytophthora ramorum. Phytopathology 2006, 96:975-981.
8. Jeffers S.N., Martin S.B. Comparison of two media selective for Phytophthora and Pythium species. Plant Dis. 1986, 70:1038-1043.
9. Kox L.F.F., van Brouwershaven I.R., van de Vossenberg B.T.L.H., van den Beld H.E., Bonants P.J.M., de Gruyter J. Diagnostic values and utility of immunological, morphological, and molecular methods for in planta detection of Phytophthora ramorum. Phytopathology 2007, 97:1119-1129.
10. Lane C.R., Hobden E., Walker L., Barton V.C., Inman A.J., Hughes K.J.D., et al. Evaluation of a rapid diagnostic field test kit for identification of Phytophthora species including P. ramorum and P. kernoviae at the point of inspection. Plant Pathol 2007, 56:828-835.
11. Rizzo D.M., Garbelotto M., Davidson J.M., Slaughter G.W., Koike S.T. Phytophthora ramorum as the cause of extensive mortality of Quercus spp. and Lithocarpus densiflorus in California. Plant Dis 2002, 86:205-214.
12. Schena L., Hughes K.J.D., Cooke D.E.L. Detection and quantification of Phytophthora ramorum, P. kernoviae, P. citricola and P. quercina in symptomatic leaves by multiplex real-time PCR. Mol. Plant Pathol 2006, 7:365-379.
13. Tomlinson J.A., Boonham N., Hughes K.J.D., Griffin R.L., Barker I. On-site DNA extraction and real-time PCR for detection of Phytophthora ramorum in the field. Appl. Environ. Microbiol. 2005, 71:6702-6710.
14. Tooley P.W., Martin F.N., Carras M.M., Frederick R.D. Real-time fluorescent polymerase chain reaction detection of Phytophthora ramorum and Phytophthora pseudosyringae using mitochondrial gene regions. Phytopathology 2006, 96:336-345.
15. Vettraino A.M., Sukno S., Vannini A., Garbelotto M. Diagnostic sensitivity and specificity of different methods used by two laboratories for the detection of Phytophthora ramorum on multiple natural hosts. Plant Pathol. doi: 2009, 10.1111/j.1365-3059.2009.02209.x.
16. Werres S., Marwitz R., Man in 't Veld W.A., De Cock W.A.M., Bonants P.J.M., De Weerdt M., et al. Phytophthora ramorum sp. nov., a new pathogen on Rhododendron and Viburnum. Mycol. Res. 2001, 105:1155-1165.