Visualization of initial bacterial colonization on dentine and enamel in situ

Journal of Microbiological Methods

Volumn 81, Issue 2, 2010, Pages 166-174

  Jung, David Jonathan ^a   Al Ahmad, Ali ^a   Follo, Marie ^a   Spitzmüller, Bettina ^a   Hoth Hannig, Wiebke ^b   Hannig, Matthias ^b   Hannig, Christian ^a,c  

^a UNIVERSITY OF FREIBURG   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

^c DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

CLSM; DAPI; Dentine; Enamel; FISH; In situ; Pellicle

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE;

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL COUNT; BACTERIUM ADHERENCE; BACTERIUM DETECTION; COLONY FORMING UNIT; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DENTIN; DESORPTION; ENAMEL; EPIFLUORESCENCE MICROSCOPE; EUBACTERIUM; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; INTERMETHOD COMPARISON; LONG TERM EXPOSURE; NONHUMAN; NORMAL HUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SCANNING ELECTRON MICROSCOPY; STREPTOCOCCUS; TOOTH FLORA; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALS; BACTERIA; BACTERIAL ADHESION; BACTERIAL PHYSIOLOGICAL PHENOMENA; CATTLE; DENTAL ENAMEL; DENTIN; FLUORESCENT DYES; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INDOLES; MICROSCOPY, ELECTRON, SCANNING; STAINING AND LABELING; TIME FACTORS;

BACTERIA (MICROORGANISMS); BOVINAE;

EID: 77951977713     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.03.002     Document Type: Article

References (42)

1. Al-Ahmad A., Wunder A., Auschill T.M., Follo M., Braun G., Hellwig E., Arweiler N.B. The in vivo dynamics of Streptococcus spp., Actinomyces naeslundii, Fusobacterium nucleatum and Veillonella spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence in situ hybridization. J. Med. Microbiol. 2007, 56:681-687.
2. Al-Ahmad A., Follo M., Selzer A.C., Hellwig E., Hannig M., Hannig C. Bacterial colonisation of enamel in situ investigated with fluorescence in situ hybridization (FISH). J. Med. Microbiol. 2009, 58(Pt 10):1359-1366.
3. Amann R.I., Ludwig W., Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 1995, 59:143-169.
4. Baume L.J. The biology of pulp and dentine. A historic, terminologic-taxonomic, histologic-biochemical, embryonic and clinical survey. Monogr. Oral. Sci. 1980, 8:1-220.
5. Beckers H.J., van der Hoeven J.S. The effects of mutual interaction and host diet on the growth rates of the bacteria Actinomyces viscosus and Streptococcus mutans during colonization of tooth surfaces in di-associated gnotobiotic rats. Arch. Oral Biol. 1984, 29:231-236.
6. Butler W.T. Matrix macromolecules of bone and dentin. Coll. Relat. Res. 1984, 4:297-307.
7. Deimling D., Breschi L., Hoth-Hannig W., Ruggeri A., Hannig C., Nekrashevych Y., Prati C., Hannig M. Electron microscopic detection of salivary alpha-amylase in the pellicle formed in situ. Eur. J. Oral Sci. 2004, 112:503-509.
8. Dige I., Nilsson H., Kilian M., Nyvad B. In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization. Eur. J. Oral Sci. 2007, 115:459-467.
9. Douglas C.W. Bacterial-protein interactions in the oral cavity. Adv. Dent. Res. 1994, 8:254-262.
10. Esser M.T.J., Marx R. Material characteristics of the hard tissues of bovine versus human teeth. Dtsch. Zahnärztl. Z. 1998, 53:713-717.
11. Fagrell T.G., Lingstrom P., Olsson S., Steiniger F., Noren J.G. Bacterial invasion of dentinal tubules beneath apparently intact but hypomineralized enamel in molar teeth with molar incisor hypomineralization. Int. J. Paediatr. Dent. 2008, 18:333-340.
12. Foster J.S., Kolenbrander P.E. Development of a multispecies oral bacterial community in a saliva-conditioned flow cell. Appl. Environ. Microbiol. 2004, 70:4340-4348.
13. Garberoglio R., Brannstrom M. Scanning electron microscopic investigation of human dentinal tubules. Arch. Oral Biol. 1976, 21:355-362.
14. Hannig M. Transmission electron microscopic study of in vivo pellicle formation on dental restorative materials. Eur. J. Oral Sci. 1997, 105:422-433.
15. Hannig M. Ultrastructural investigation of pellicle morphogenesis at two different intraoral sites during a 24-h period. Clin. Oral Investig. 1999, 3:88-95.
16. Hannig M., Hannig C. Does a biofilm free of bacteria, exist in situ?. J. Parodontol. Implantol. Orale 2007, 26:187-200.
17. Hannig C., Hannig M. The oral cavity - a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin. Oral Investig. 2009, 13:123-139.
18. Hannig M., Joiner A. The structure, function and properties of the acquired pellicle. Monogr. Oral Sci. 2006, 19:29-64.
19. Hannig C., Attin T., Hannig M., Henze E., Brinkmann K., Zech R. Immobilisation and activity of human alpha-amylase in the acquired enamel pellicle. Arch. Oral Biol. 2004, 49:469-475.
20. Hannig M., Fiebiger M., Güntzer M., Döbert A., Zimehl R., Nekrashevych Y. Protective effect of the in situ formed short-term salivary pellicle. Arch. Oral Biol. 2004, 49:903-910.
21. Hannig C., Hoch J., Becker K., Hannig M., Attin T. Lysozyme activity in the initially formed in situ pellicle. Arch. Oral Biol. 2005, 50:821-828.
22. Hannig M., Khanafer A.K., Hoth-Hannig W., Al-Marrawi F., Acil Y. Transmission electron microscopy comparison of methods for collecting in situ formed enamel pellicle. Clin. Oral Investig. 2005, 9:30-37.
23. Hannig C., Becker K., Häusler N., Hoth-Hannig W., Attin T., Hannig M. Protective effect of the in situ pellicle on dentin erosion - an ex vivo pilot study. Arch. Oral Biol. 2007, 52:444-449.
24. Hannig C., Hannig M., Rehmer O., Braun G., Hellwig E., Al-Ahmad A. Fluorescence microscopic visualization and quantification of initial bacterial colonization on enamel in situ. Arch. Oral Biol. 2007, 52:1048-1056.
25. Jones I.L., Leaver A.G. Studies on the minor components of the organic matrix of human dentine. Arch. Oral Biol. 1974, 19:371-380.
26. Kolenbrander P.E., Andersen R.N., Blehert D.S., Egland P.G., Foster J.S., Palmer R.J. Communication among oral bacteria. Microbiol. Mol. Biol. Rev. 2002, 66:486-505.
27. La Fleche R.G., Frank R.M., Steuer P. The extent of the human odontoblast process as determined by transmission electron microscopy: the hypothesis of a retractable suspensor system. J. Biol. Buccale 1985, 13:293-305.
28. Love R.M. The effect of tissue molecules on bacterial invasion of dentine. Oral Microbiol. Immunol. 2002, 17:32-37.
29. Love R.M. Bacterial adhesins - their role in tubule invasion and endodontic disease. Aust. Endod. J. 2002, 28:25-28.
30. Marsh P.D. Antimicrobial strategies in the prevention of dental caries. Caries Res. 1993, 27(Suppl 1):72-76.
31. Marsh P.D. Dental plaque: biological significance of a biofilm and community life-style. J. Clin. Periodontol. 2005, 32(Suppl 6):7-15.
32. Moore W.E., Moore L.V. The bacteria of periodontal diseases. Periodontology 2000 1994, 5:66-77.
33. Nakamichi I., Iwaku M., Fusayama T. Bovine teeth as possible substitutes in the adhesion test. J. Dent. Res. 1983, 62:1076-1081.
34. Nyvad B., Fejerskov O. Transmission electron microscopy of early microbial colonization of human enamel and root surfaces in vivo. Scand. J. Dent. Res. 1987, 95:297-307.
35. Nyvad B., Fejerskov O. Scanning electron microscopy of early microbial colonization of human enamel and root surfaces in vivo. Scand. J. Dent. Res. 1987, 95:287-296.
36. Nyvad B., Kilian M. Microbiology of the early colonization of human enamel and root surfaces in vivo. Scand. J. Dent. Res. 1987, 95:369-380.
37. Nyvad B., Kilian M. Comparison of the initial streptococcal microflora on dental enamel in caries-active and in caries-inactive individuals. Caries Res. 1990, 24:267-272.
38. Paster B.J., Boches S.K., Galvin J.L., Ericson R.E., Lau C.N., Levanos V.A., Sahasrabudhe A., Dewhirst F.E. Bacterial diversity in human subgingival plaque. J. Bacteriol. 2001, 183:3770-3783.
39. Scannapieco F.A. Saliva-bacterium interactions in oral microbial ecology. Crit. Rev. Oral Biol. Med. 1994, 5:203-248.
40. Schwartz T., Hoffmann S., Obst U. Formation of natural biofilms during chlorine dioxide and u.v. disinfection in a public drinking water distribution system. J. Appl. Microbiol. 2003, 95:591-601.
41. Teughels W., Van Assche N., Sliepen I., Quirynen M. Effect of material characteristics and/or surface topography on biofilm development. Clin. Oral Implants. Res. 2006, 17(Suppl 2):68-81.
42. Thomas H.F. The dentin-predentin complex and its permeability: anatomical overview. J. Dent. Res. 1985, 64:607-612. Spec No.