Evaluation of the MTT lymphocyte proliferation assay for the diagnosis of neurocysticercosis

Journal of Microbiological Methods

Volumn 81, Issue 2, 2010, Pages 175-178

  Verma, Avantika ^a   Prasad, Kashi N ^a   Singh, Aloukick K ^a   Nyati, Kishan K ^a   Gupta, Rakesh K ^a   Paliwal, Vimal K ^a  

^a SANJAY GANDHI POSTGRADUATE INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Lymphocytes; Magnetic resonance imaging; MTT; Neurocysticercosis; Taenia solium

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; PARASITE ANTIGEN; TETRAZOLIUM;

ARTICLE; CELL ISOLATION; CLINICAL EVALUATION; COLORIMETRY; CONTROLLED STUDY; CYST FLUID; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; EPILEPSY; HUMAN; LYMPHOCYTE PROLIFERATION; MAJOR CLINICAL STUDY; NEUROCYSTICERCOSIS; NEUROIMAGING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE IMAGING; PRIORITY JOURNAL; PROCESS DEVELOPMENT; SENSITIVITY AND SPECIFICITY; SEROLOGY; TAENIA SOLIUM; TISSUE CULTURE;

ANIMALS; CELL PROLIFERATION; FEMALE; HUMANS; IMMUNOASSAY; LYMPHOCYTES; MALE; NEUROCYSTICERCOSIS; SENSITIVITY AND SPECIFICITY; TAENIA SOLIUM; TETRAZOLIUM SALTS; THIAZOLES;

TAENIA SOLIUM;

EID: 77951974553     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.03.001     Document Type: Article

References (39)

1. Arnould R., Dubois J. Comparison of two cytotoxicity assays, tetrazolium derivative reduction (MTT) and tritiated thymidine uptake on the malignant cell lines using chemotherapeutic agents. Cancer Res. 1999, 10:145.
2. Biswas R., Parija S.C., Narayan S.K. Dot ELISA for the diagnosis of neurocysticercosis. Rev. Inst. Med.Trop Sao. Paulo. 2004, 46:249-252.
3. Blanca R.I., Maria I.A., Peter C.M., Teale J.M. Analysis of the peripheral immune response in patients with neurocysticercosis: evidence for T cell reactivity to parasite glycoprotein and vesicular fluid antigens. Am. J. Trop. Med. Hyg. 2001, 65:366-370.
4. Bueno E.C., Vaz A.J., Machado L.R., Livramento J.A., Avila S.L.M., Ferreira A.W. Antigen-specific suppression of cultured lymphocytes from patients with neurocysticercosis. Clin. Exp. Immunol. 2001, 126:304-310.
5. Carpio A., Escobar A., Hauser W.A. Cysticercosis and epilepsy: a critical review. Epilepsia 1998, 39:1025-1040.
6. Comely J.C., Turner C.H. Potential of a soluble tetrazolium/formazan assay for the evaluation of filarial viability. Int. J. Parasitol. 1990, 20:251-255.
7. Correa D.A., Tovar B.E., Plancarte A., Flisser A. Cisticercosis humana: Relación inmunológica huésped-parásito. Cisticercosis humana y porcina, su conocimiento e investigación en México 1989, 31-43. Limusa-Noriega, México D.F, México. A. Flisser, F. Malagon (Eds.).
8. Cruz M.E., Schantz P.M., Cruz I., Espinosa P., Preux P.M., Cruz A., Benitez W., Tsang V.C.W., Fermosos J., Dumas M. Epilepsy and neurocysticercosis in an Andean community. Int. J. Epidemiol. 1999, 28:799-803.
9. Del Brutto O.H., Rajshekhar V., White A.C., Tsang V.C., Nash T.E., Takayanagui O.M., Schantz P.M., Evans C.A., Flisser A., Correa D., Botero D., Allan J.C., Sarti E., Gonzalez A.E., Gilman R.H., Garcia H.H. Proposed diagnostic criteria for neurocysticercosis. Neurology 2001, 57:177-183.
10. Ferrari M., Fornasiero M.C., Isetta A.M. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J. Immunol. Methods 1990, 131:165-172.
11. Garcia-Noval J., Allan J.C., Fledes C., Moreno E., DeMata F., Torres-Alvarez R., Soto de Alfaro H., Yurrita P., Higueros-Morales H., Mencos F., Craig P.S. Epidemiology of Taenia solium taeniasis and cysticercosis in two rural Guatemalan communities. Am. J. Trop. Med. Hyg. 1996, 55:282-289.
12. 3H] thymidine incorporation with MTT and MTS based bioassays for human and murine IL-2 and IL-4 analysis. Tetrazolium assays provide markedly enhanced sensitivity. J. Immunol. Methods 1995, 187:85-93.
13. Gonzalez A.E., Gauci C.G., Barber D., Gilman R.H., Tsang V.C., Garcia H.H., Verastegui M., Lightowlers M.W. Vaccination of pigs to control human neurocysticercosis. Am. J. Trop. Med. Hyg. 2005, 72:837-839.
14. Hayon T., Dvilansky A., Shpilberg O., Nathan I. Appraisal of the MTT-based assay as a useful tool for predicting drug chemosensitivity in leukemia. Leuk. Lymphoma. 2003, 44:1957-1962.
15. Leslie H., Hay F.C. Practical Immunology 1989, 429. Blackwell Scientific Publication, Oxford. third ed.
16. Meeusen E., Gorrell M.D., Rickard M.D., Brandon M.R. Lymphocyte subpopulations of sheep in protective immunity to Taenia hydatigena. Parasite Immunol. 1989, 11:69-81.
17. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65:55-63.
18. Nikes M., Otto M. Towards an optimized MTT assay. J. Immunol. Methods 1990, 130:149-151.
19. Nyati K.K., Prasad K.N., Rizwan A., Verma A., Paliwal V.K., Pradhan S. Lymphocyte transformation test detects a response to Campylobacter jejuni antigens in patients with Guillain-Barré syndrome. Med. Microbiol. Immunol. 2010, 10.1007/s00430-010-0144-3.
20. Pardini A.X., Vaz A.J., Machado L.D.R., Livramento J.A. Cysticercus antigens in cerebrospinal fluid samples from patients with neurocysticercosis. J. Clin. Microbiol. 2001, 39:3368-3372.
21. Prabhakaran V., Rajshekhar V., Murrell K.D., Oommen A. Taenia solium metacestode glycoproteins as diagnostic antigens for solitary cysticercus granuloma in Indian patients. Trans. R. Soc. Trop. Med. Hyg. 2004, 98:478-484.
22. Prasad A., Prasad K.N., Yadav A., Gupta R.K., Pradhan S., Jha S., Tripathi M., Husain M. Lymphocyte transformation test: a new method for diagnosis of neurocysticercosis. Diagn. Microbiol. Infect. Dis. 2008, 61:198-202.
23. Price P., McMiller T.J. Use of the tetrazolium assay in measuring the response of human tumors cell to ionizing radiation. Cancer Res. 1990, 50:1392-1396.
24. Rahalkar M.D., Shetty D.D., Kelkar A.B., Kelkar A.A., Kinare A.S., Ambardekar S.T. The many faces of cysticercosis. Clin. Radiol. 2000, 55:668-674.
25. Rajshekhar V., Raghava M.V., Prabhakaran V., Oommen A., Muliyil J. Active epilepsy as an index of burden of neurocysticercosis in Vellore district, India. Neurology 2006, 67:2135-2139.
26. Ramos-Kuri M., Montoya R.M., Padilla A., Govezensky T., Díaz M.L., Sciutto E., Sotelo J., Larralde C. Immunodiagnosis of neurocysticercosis: disappointing performance of serology (enzyme-linked immunosorbent assay) in an unbiased sample of neurological patients. Arch. Neurol. 1992, 49:633-636.
27. Restrepo B.I., Aguilar M.I., Melby P.C., Teale J.M. Analysis of the peripheral immune response in patients with neurocysticercosis: evidence for T cell reactivity to parasite glycoprotein and vesicular fluid antigens. Am. J. Trop. Med. Hyg. 2001, 65:366-370.
28. Robinson P., Atmar R.L., Lewis D.E., White A.C. Granuloma cytokines in murine cysticercosis. Infect. Immun. 1997, 65:2925-2931.
29. Rodriguez S., Dorny P., Tsang V.C., Pretell E.J., Brandt J., Lescano A.G., Gonzalez A.E., Gilman R.H., Garcia H.H. Detection of Taenia solium antigens and anti-T. solium antibodies in paired serum and cerebrospinal fluid samples from patients with intraparenchymal or extraparenchymal neurocysticercosis. J. Infec. Dis. 2009, 199:1345-1352. Cysticercosis Working Group in Peru.
30. Sahu P.S., Parija S.C., Narayan S.K., Kumar D. Evaluation of an IgG-ELISA strategy using Taenia solium metacestode somatic and excretory-secretory antigens for diagnosis of neurocysticercosis revealing biological stage of the larvae. Acta Trop. 2009, 110:38-45.
31. Sanchez A.L., Ljungstrom I., Medina M.T. Diagnosis of human neurocysticercosis in endemic countries: a clinical study in Honduras. Parasitol. Int. 1999, 48:81-89.
32. Sciutto E., Fragoso G., Baca M., De la Cruz V., Lemus L., Lamoyi E. Depressed T-cell proliferation associated with susceptibility to experimental Taenia crassiceps infection. Infect. Immun. 1995, 63:2277-2281.
33. Shimoyama Y., Kubota T., Watanabe M., Ishibiki K., Abe O. Predictability of in vivo chemosensitivity by in vitro MTT assay with reference to the clonogenic assay. J. Surg. Oncol. 1989, 41:12-18.
34. Singh G., Kaushal V., Ram S., Kaushal R.K., Dhanuka A.K., Khurana S. Cysticercus immunoblot assay in patients with single small enhancing lesions and multilesional cysticercosis. J. Assoc. Physicians India 1999, 47:476-479.
35. Tsang V.C., Brand J.A., Boyer A.E. An enzyme-linked immunoeletrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J. Infect. Dis. 1989, 159:50-59.
36. Ulukaya E., Ozdikicioglu F., Oral A.Y., Demirci M. The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested. Toxicol. In Vitro 2008, 22:232-239.
37. Weichert H., Blechshmidt I., Schroder S., Ambrosius H. The MTT assay as a rapid test for cell proliferation and cell killing: application to human peripheral blood lymphocytes (PBL). Allerg. Immunol. 1991, 37:139-144.
38. White A.C. Neurocysticercosis: a major cause of neurological disease worldwide. Clin. Infect. Dis. 1997, 24:101-103.
39. Wilson M., Bryan R.T., Fried J.A., Ware D.A., Schantz P.M., Pilcher J.B., Tsang V.C. Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J. Infect. Dis. 1991, 164:1007-1009.