Low-Level Expression of a Folding-Incompetent Protein in Escherichia coli: Search for the Molecular Determinants of Protein Aggregation In Vivo

Journal of Molecular Biology

Volumn 398, Issue 4, 2010, Pages 600-613

  Winkelmann, Julia ^a   Calloni, Giulia ^b   Campioni, Silvia ^a   Mannini, Benedetta ^a   Taddei, Niccolò ^a   Chiti, Fabrizio ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Heterologous; Intrinsic aggregation propensity; Oligomers; Protein misfolding; Simulations

Indexed keywords

AMYLOID; ESCHERICHIA COLI PROTEIN; PROTEIN HYPF; UNCLASSIFIED DRUG; HYPF PROTEIN, E COLI; MUTANT PROTEIN; TRANSFERASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BETA SHEET; CONTROLLED STUDY; ESCHERICHIA COLI; HYDROPHOBICITY; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR RECOGNITION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN FOLDING; WESTERN BLOTTING; WILD TYPE; CHEMISTRY; GENE EXPRESSION; GENETICS; METABOLISM; METHODOLOGY; PROTEIN BINDING; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

AMYLOID; BLOTTING, WESTERN; CARBOXYL AND CARBAMOYL TRANSFERASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; MUTANT PROTEINS; PROTEIN BINDING; PROTEIN FOLDING; SOLUBILITY;

EID: 77951887037     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.03.030     Document Type: Article

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