Alternative splicing in exon 9 of glucocorticoid receptor pre-mRNA is regulated by SRp40

Molecular Biology Reports

Volumn 37, Issue 3, 2010, Pages 1427-1433

  Yan, Xue Bo ^a,b   Tang, Chen Hong ^a   Huang, Yan ^a   Fang, Hui ^b   Yu, Zhi Qiang ^b   Wu, Li Min ^b   Liu, Rong Yu ^a  

^a ANHUI MEDICAL UNIVERSITY   (China)

^b UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

Alternative splicing; Exon; Glucocorticoid receptor; Minigene; SR proteins; Transfection

Indexed keywords

ARGININE RICH PROTEIN; CELL PROTEIN; GENOMIC DNA; GLUCOCORTICOID RECEPTOR; GLUCOCORTICOID RECEPTOR ALPHA; GLUCOCORTICOID RECEPTOR BETA; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1; MESSENGER RNA PRECURSOR; PROTEIN SRP40; SERINE RICH PROTEIN; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; NUCLEAR PROTEIN; PRIMER DNA; RNA BINDING PROTEIN; RNA PRECURSOR; SERINE ARGININE RICH SPLICING PROTEINS; SERINE-ARGININE-RICH SPLICING PROTEINS;

ALTERNATIVE RNA SPLICING; ARTICLE; CONTROLLED STUDY; EXON; GENE SILENCING; GENETIC REGULATION; GENETIC TRANSFECTION; GENOME SIZE; HELA CELL; HUMAN; PROTEIN ANALYSIS; PROTEIN INDUCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; CELL LINE; GENETICS; METABOLISM; PHYSIOLOGY;

ALTERNATIVE SPLICING; CELL LINE; DNA PRIMERS; EXONS; HUMANS; NUCLEAR PROTEINS; RECEPTORS, GLUCOCORTICOID; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA PRECURSORS; RNA, SMALL INTERFERING; RNA-BINDING PROTEINS; TRANSFECTION;

EID: 77951882147     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9529-z     Document Type: Article

References (22)

1. Bamberger CM, Bamberger AM, de Castro M, Chrousos GP (1995) Glucocorticoid receptor beta, a potential endogenous inhibitor of glucocorticoid action in humans. J Clin Invest 95(6):2435-2441
2. Fruchter O, Kino T, Zoumakis E, Alesci S, De Martino M, Chrousos G, Hochberg Z (2005) The human glucocorticoid receptor (GR) isoform beta differentially suppresses GR{alpha}- induced transactivation stimulated by synthetic glucocorticoids. J Clin Endocrinol Metab 90(6):3505-3509
3. Leung DY, Hamid Q, Vottero A, Szefler SJ, Surs W, Minshall E, Chrousos GP, Klemm DJ (1997) Association of glucocorticoid insensitivity with increased expression of glucocorticoid receptor beta. J Exp Med 186(9):1567-1574
4. Oakley RH, Jewell CM, Yudt MR, Bofetiado DM, Cidlowski JA (1999) The dominant negative activity of the human glucocorticoid receptor beta isoform. Specificity and mechanisms of action. J Biol Chem 274(39):27857-27866
5. Oakley RH, Sar M, Cidlowski JA (1996) The human glucocorticoid receptor beta isoform. Expression, biochemical properties, and putative function. J Biol Chem 271(16):9550-9559
6. Encio IJ, Detera-Wadleigh SD (1991) The genomic structure of the human glucocorticoid receptor. J Biol Chem 266(11):7182-7188
7. Hauk PJ, Goleva E, Strickland I, Vottero A, Chrousos GP, Kisich KO, Leung DY (2002) Increased glucocorticoid receptor Beta expression converts mouse hybridoma cells to a corticosteroidinsensitive phenotype. Am J Respir Cell Mol Biol 27(3):361-367
8. Christodoulopoulos P, Leung DY, Elliott MW, Hogg JC, Muro S, Toda M, Laberge S, Hamid QA (2000) Increased number of glucocorticoid receptor-beta-expressing cells in the airways in fatal asthma. J Allergy Clin Immunol 106(3):479-484
9. HamidQA, Wenzel SE,Hauk PJ, TsicopoulosA,WallaertB, Lafitte JJ, Chrousos GP, Szefler SJ, Leung DY (1999) Increased glucocorticoid receptor beta in airway cells of glucocorticoid-insensitive asthma. Am J Respir Crit Care Med 159(5 Pt 1):1600-1604
10. Hamilos DL, Leung DY, Muro S, Kahn AM, Hamilos SS, Thawley SE, Hamid QA (2001) GRbeta expression in nasal polyp inflammatory cells and its relationship to the anti-inflammatory effects of intranasal fluticasone. J Allergy Clin Immunol 108(1):59-68
11. Honda M, Orii F, Ayabe T, Imai S, Ashida T, Obara T, Kohgo Y (2000) Expression of glucocorticoid receptor beta in lymphocytes of patients with glucocorticoid-resistant ulcerative colitis. Gastroenterology 118(5):859-866
12. Caceres JF, Stamm S, Helfman DM, Krainer AR (1994) Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. Science 265(5179):1706-1709
13. Hanamura A, Caceres JF, Mayeda A, Franza BR Jr, Krainer AR (1998) Regulated tissue-specific expression of antagonistic premRNA splicing factors. RNA 4(4):430-444
14. Pollard AJ, Krainer AR, Robson SC, Europe-Finner GN (2002) Alternative splicing of the adenylyl cyclase stimulatory G-protein G alpha(s) is regulated by SF2/ASF and heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) and involves the use of an unusual TG 30-splice Site. J Biol Chem 277(18):15241-15251
15. Tyson-Capper AJ, Bailey J, Krainer AR, Robson SC, Europe- Finner GN (2005) The switch in alternative splicing of cyclic AMP-response element modulator protein CREM{tau}2{alpha} (activator) to CREM{alpha} (repressor) in human myometrial cells is mediated by SRp40. J Biol Chem 280(41):34521-34529
16. Xu Q, Leung DY, Kisich KO (2003) Serine-arginine-rich protein p30 directs alternative splicing of glucocorticoid receptor premRNA to glucocorticoid receptor beta in neutrophils. J Biol Chem 278(29):27112-27118
17. Ladd AN, Nguyen NH, Malhotra K, Cooper TA (2004) CELF6, a member of the CELF family of RNA-binding proteins, regulates muscle-specific splicing enhancer-dependent alternative splicing. J Biol Chem 279(17):17756-17764
18. Lee KY, Chamberlin ME, Horodyski FM (2002) Biological activity of Manduca sexta allatotropin-like peptides, predicted products of tissue-specific and developmentally regulated alternatively spliced mRNAs. Peptides 23(11):1933-1941
19. Watanuki T, Funato H, Uchida S, Matsubara T, Kobayashi A, Wakabayashi Y, Otsuki K, Nishida A, Watanabe Y (2008) Increased expression of splicing factor SRp20 mRNA in bipolar disorder patients. J Affect Disord 110(1-2):62-69
20. Zhu J, Gong JY, Goodman OB Jr, Cartegni L, Nanus DM, Shen R (2007) Bombesin attenuates pre-mRNA splicing of glucocorticoid receptor by regulating the expression of serine-arginine protein p30c (SRp30c) in prostate cancer cells. Biochim Biophys Acta 1773(7):1087-1094
21. Turner JD, Muller CP (2005) Structure of the glucocorticoid receptor (NR3C1) gene 50 untranslated region: Identification, and tissue distribution of multiple new human exon 1. J Mol Endocrinol 35(2):283-292
22. Russcher H, Dalm VA, de Jong FH, Brinkmann AO, Hofland LJ, Lamberts SW, Koper JW (2007) Associations between promoter usage and alternative splicing of the glucocorticoid receptor gene. J Mol Endocrinol 38(1-2):91-98