A simple adhesion assay for studying interactions between platelets and endothelial cells in vitro

Cytotechnology

Volumn 62, Issue 1, 2010, Pages 17-22

  Xu, Xian Xiang ^a   Gao, Xing Hua ^a   Pan, Rong ^a   Lu, Dan ^a   Dai, Yue ^a  

^a CHINA PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Adhesion assay; Endothelial cell; Methodology; Platelet; Sudan Black B

Indexed keywords

ACIDIC FIBROBLAST GROWTH FACTOR; HEPARIN;

ABSORPTION; ARTICLE; CELL ASSAY; CELL INTERACTION; CELL LYSATE; CONTROLLED STUDY; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; MONOLAYER CULTURE; SPECTROPHOTOMETER; STAINING; THROMBOCYTE ADHESIVENESS; THROMBOCYTE COUNT;

EID: 77951878998     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-010-9256-2     Document Type: Article

References (22)

1. GA Ackerman 1952 A modification of the Sudan Black B technique for the possible cytochemical demonstration of masked lipids Science 115 629 663 10.1126/science.115.2997.629 1:CAS:528:DyaG38XksFGqtQ%3D%3D
2. RK Andrews MC Berndt 2004 Platelet physiology and thrombosis Thromb Res 114 447 453 10.1016/j.thromres.2004.07.020 1:CAS:528:DC%2BD2cXovVKms7o%3D (Pubitemid 39422578)
3. P Bellavite G Andrioli P Guzzo P Arigliano S Chirumbolo F Manzato C Santonastaso 1994 A colorimetric method for the measurement of platelet adhesion in microtiter plates Anal Biochem 216 444 450 10.1006/abio.1994.1066 1:CAS:528:DyaK2cXhtVWjs7c%3D (Pubitemid 24076032)
4. L Belyanskaya P Manser P Spohn A Bruinink P Wick 2007 The reliability and limits of the MTT reduction assay for carbon nanotubes-cell interaction Carbon 45 2643 2648 10.1016/j.carbon.2007.08.010 1:CAS:528:DC%2BD2sXht1ShtL7K (Pubitemid 47588185)
5. DB Cines ES Pollak CA Buck J Loscalzo GA Zimmerman RP McEver JS Pober TM Wick BA Konkle BS Schwartz ES Barnathan KR McCrae BA Hug AM Schmidt DM Stern 1998 Endothelial cells in physiology and in the pathophysiology of vascular disorders Blood 91 3527 3561 1:CAS:528:DyaK1cXjtFersr8%3D
6. KD Curwen HY Kim M Vazquez RI Handin MA Gimbrone Jr 1982 Platelet adhesion to cultured vascular endothelial cells. A quantitative monolayer adhesion assay J Lab Clin Med 100 425 436 1:STN:280:DyaL383mslGmuw%3D%3D
7. RL Czervionke JC Hoak GL Fry 1978 Effect of aspirin on thrombin induced adherence of platelets to cultured endothelial cells from the vessel wall J Clin Invest 62 841 856 10.1172/JCI109197
8. JA Davies VC Menys 1982 Effect of heparin on platelet monolayer adhesion, aggregation and production of malondialdehyde Thromb Res 26 31 41 10.1016/0049-3848(82)90148-7 1:CAS:528:DyaL38XktFKqsrw%3D (Pubitemid 12123206)
9. RJ Gonzalez JB Tarlor 2001 Evaluation of hepatic subcellular fractions for Almar blue and MTT reductase activity Toxicol In Vitro 15 257 259 10.1016/S0887-2333(01)00014-5 1:CAS:528:DC%2BD3MXjslSitrk%3D (Pubitemid 32476835)
10. S Holme A Heaton J Roodt 1993 Concurrent label method with 111In and 51Cr allows accurate evaluation of platelet viability of stored platelet concentrates Br J Haematol 84 717 723 10.1111/j.1365-2141.1993.tb03151.x 1:STN:280:DyaK2c%2FjtFygsQ%3D%3D
11. EA Jaffe RL Nachman CG Becker CR Minick 1973 Culture of human endothelial cells derived from umbilical veins. Identification by morphologic criteria J Clin Invest 52 2745 10.1172/JCI107470 1:STN:280:DyaE2c%2FitFKgug%3D%3D
12. JE Kaplan DG Moon LK Weston FL Minnear PJ Del Vecchio JM Shepard JW Fenton 2nd 1989 Platelets adhere to thrombin treated endothelial cells in vitro Am J Physiol 257 H423 H433 1:CAS:528:DyaL1MXlslWlsbY%3D
13. CH Liao JT Cheng CM Teng 2005 Interference of neutrophil-platelet interaction by YC-1: a cGMP-dependent manner on heterotypic cell-cell interaction Eur J Pharmacol 519 157 158 10.1016/j.ejphar.2005.06.041 1:CAS:528:DC%2BD2MXpslyms78%3D (Pubitemid 43181179)
14. S Liebner U Cavallaro E Dejana 2006 The multiple languages of endothelial cell-to-cell communication Arterioscler Thromb Vasc Biol 26 1431 1438 10.1161/01.ATV.0000218510.04541.5e 1:CAS:528:DC%2BD28XmvFyjtL8%3D (Pubitemid 44288966)
15. S Massberg G Enders R Leiderer S Eisenmenger D Vestweber F Krombach K Messmer 1998 Platelet-endothelial cell interactions during ischemia/reperfusion: the role of P-selectin Blood 92 507 515 1:CAS:528:DyaK1cXksFaru7Y%3D
16. Rose NR, Friedman H, Fahey JL (1986) Manual of clinical laboratory immunology, 3rd ed edn. American Society for Microbiology, Washington, DC, pp 226-235
17. WI Rosenblum 1997 Platelet adhesion and aggregation without endothelial denudation or exposure of basal lamina and/or collagen J Vasc Res 34 409 417 10.1159/000159251 1:CAS:528:DyaK2sXotFShtrw%3D
18. ZM Ruggeri 2009 Platelet adhesion under flow Microcirculation 16 58 83 10.1080/10739680802651477 1:CAS:528:DC%2BD1MXjvVGjsrc%3D
19. T Szuwart T Brzoska TA Luger T Filler E Peuker R Dierichs 2000 Vitamin E reduces platelet adhesion to human endothelial cells in vitro Am J Hematol 65 1 4 10.1002/1096-8652(200009)65:1<1::AID-AJH1>3.0.CO;2-8 1:CAS:528:DC%2BD3cXmsFSksLc%3D (Pubitemid 30659967)
20. MS Thakur SG Prapulla NG Karanth 1989 Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B Enzyme Microb Technol 11 252 254 10.1016/0141-0229(89)90101-4 1:CAS:528:DyaL1MXhvFWisLs%3D (Pubitemid 19097698)
21. Warkentin TE, Aird WC, Rand JH (2003) Platelet-endothelial interactions: sepsis, HIT, and antiphospholipid syndrome. Hematology 2003:497-519
22. M Wei G Tai Y Gao N Li B Huang Y Zhou S Hao X Zeng 2004 Modified heparin inhibits P-selectin-mediated cell adhesion of human colon carcinoma cells to immobilized platelets under dynamic flow conditions J Biol Chem 279 29202 29210 10.1074/jbc.M312951200 1:CAS:528:DC%2BD2cXlsVCmsrc%3D (Pubitemid 38915794)