Use of 2-D electrophoresis and ESI mass spectrometry techniques to characterize Fusobacterium nucleatum proteins up-regulated after oxidative stress

Anaerobe

Volumn 16, Issue 2, 2010, Pages 179-182

  Silva, Vania L ^a   Diniz, Claudio G ^a   Santos, Simone G ^a   Carvalho, Maria Auxiliadora R ^a   Farias, Luiz M ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PROTEOME; VIRULENCE FACTOR;

ADAPTATION; AEROBIC METABOLISM; ARTICLE; BACTERIAL GENOME; BACTERIAL GROWTH; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; FAMILIA; FUSOBACTERIACEAE; FUSOBACTERIUM NUCLEATUM; GENE SEQUENCE; GENUS; GRAM NEGATIVE ANAEROBIC BACTERIA; HOST PATHOGEN INTERACTION; MOUTH FLORA; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; TWO DIMENSIONAL ELECTROPHORESIS; UPREGULATION;

BACTERIAL PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FUSOBACTERIUM NUCLEATUM; GENE EXPRESSION PROFILING; OXIDATIVE STRESS; PROTEOME; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STRESS, PHYSIOLOGICAL;

BACTERIA (MICROORGANISMS); FUSOBACTERIACEAE; FUSOBACTERIUM; FUSOBACTERIUM NUCLEATUM; NEGIBACTERIA;

EID: 77951768637     PISSN: 10759964     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2009.08.001     Document Type: Article

References (20)

1. Garrity G.M., Bell J.A., Lilburn T.G. Taxonomic outline of the prokaryotes Bergey's manual of systematic bacteriology 2004, Springer, New York, pp. 320-321. 2nd ed.
2. Brook I. The role of anaerobic bacteria in sinusitis. Anaerobe 2006, 12:5-12.
3. Perea E.J. Oral flora in the age of molecular biology. Med Oral Patol Oral Cir Bucal 2004, 9(Suppl.):6-10.
4. Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., et al. Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586. J Bacteriol 2002, 184:2005-2018.
5. Farias F.F., Lima F.L., Carvalho M.A.R., Nicoli J.R., Farias L.M. Influence of isolation site, laboratory handling and growth stage on oxygen tolerance of Fusobacterium strains. Anaerobe 2001, 7:271-276.
6. Rocha E.R., Owens J.R.G., Smith C.J. The redox-sensitive transcriptional activator OxyR regulates the peroxide response regulon in the obligate anaerobe Bacteroides fragilis. J Bacteriol 2000, 182:5059-5069.
7. Imlay J.A. Pathways of oxidative damage. Annu Rev Microbiol 2003, 57:395-418.
8. Silva V.L., Diniz C.G., Cara D.C., Santos S.G., Nicoli J.R., Carvalho M.A.R., et al. Enhanced pathogenicity of Fusobacterium nucleatum adapted to oxidative stress. Microb Pathog 2005, 39:131-138.
9. Silva V.L., Diniz C.G., Santos S.G., Gomes R.M.F., Nicoli J.R., Magalhães P.P., et al. Physiological alterations of a Fusobacterium nucleatum strain exposed to oxidative stress. J Appl Microbiol 2007, 103:20-26.
10. Shah H.N., Keys C.J., Schmid O., Gharbia S.E. Matrix-assisted laser desorption/ionization time-of-flight mass spectometry and proteomics: a new era in anaerobic microbiology. Clin Infect Dis 2002, 35(Suppl. 1):59-64.
11. Tyers M., Mann M. From genomics to proteomics. Nature 2003, 422:193-197.
12. O'Farrell P.H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975, 250:4007-4021.
13. Diniz C.G., Farias L.M., Carvalho M.A.R., Rocha E.R., Smith C.J. Differential gene expression in a Bacteroides fragilis metronidazole-resistant mutant. J Antimicrob Chemother 2004, 54:100-108.
14. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
15. Pimenta A.M.C., Rates B., Bloch C., Gomes P.C., Santoro M.M., De Lima M.E., et al. Electrospray ionization quadrupole time-of-flight and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometric analyses to solve micro-heterogeneity in post-translationally modified peptides from Phoneutria nigriventer (Aranea, Ctenidae) venom. Rapid Commun Mass Spectrom 2005, 19:31-37.
16. Wemekamp-Kamphuis H.H., Wouters J.A., de Leeuw P.L.A., Hain T., Chakraborty T., Abee T. Identification of sigma factor B-controlled genes and their impact on acid stress, high hydrostatic pressure and freeze survival in Listeria monocytogenes. Appl Environ Microbiol 2004, 70:3457-3466.
17. Botsford J.L., Alvarez M., Hernandez R., Nichols R. Accumulation of glutamate by Salmonella typhimurium in response to osmotic stress. Appl Environ Microbiol 1994, 60:2568-2574.
18. Spector M.P., DiRusso C.C., Pallen M.J., del Portillo F.G., Dougan G., Finlay B.B. The medium/long-chain fatty acyl-CoA dehydrogenase (fadF) gene of Salmonella typhimurium is a phase 1 starvation-stress response (SSR) locus. Microbiology 1999, 145:15-31.
19. Paron I., D'Élia A., D'Ambrosio C., Sacaloni A., D'Aurizio F., Prescott A., et al. A proteomic approach to identify early molecular targets of oxidative stress in human epithelial lens cells. Biochem J 2004, 378:929-937.
20. Kilstrup M., Jacobsen M., Hammer K., Vogensen F.K. Induction of heat shock proteins DnaK, GroEL and GroES by salt stress in Lactococcus lactis. Appl Environ Microbiol 1997, 63:1826-1837.