Design of a multiplex PCR assay for the simultaneous detection and confirmation of Neisseria gonorrhoeae

Journal of Clinical Pathology

Volumn 63, Issue 5, 2010, Pages 431-433

  O'Callaghan, Isabelle ^a   Corcoran, Daniel ^a   Lucey, Brigid ^a  

^a University College Cork and INFANT Centre   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GENE SEQUENCE; GENE TARGETING; MULTIPLEX POLYMERASE CHAIN REACTION; NEISSERIA GONORRHOEAE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIBRIO CHOLERAE;

DNA, BACTERIAL; GENES, BACTERIAL; GONORRHEA; HUMANS; NEISSERIA GONORRHOEAE; POLYMERASE CHAIN REACTION; PORINS; SENSITIVITY AND SPECIFICITY;

EID: 77951664284     PISSN: 00219746     EISSN: 14724146     Source Type: Journal    
DOI: 10.1136/jcp.2009.071431     Document Type: Article

References (16)

1. Cornelissen CN. Identification and characterization of gonococcal iron transport systems as potential vaccine antigens. Future Microbiol 2008;3:287-298
2. Diemert DJ, Libman MD, Lebel P. Confirmation by 16S rRNA PCR of the COBAS AMPLICOR CT/NG test for diagnosis of Neisseria gonorrhoeae infection in a low-prevalence population. J Clin Microbiol 2002;40:4056-4059 (Pubitemid 35285789)
3. Young H, Manavi K, Mc Millan A. Evaluation of ligase chain reaction for the non-cultural detection of rectal and pharyngeal gonorrhoea in men who have sex with men. Sex Transm Infec 2003;79:484-486 (Pubitemid 38021410)
4. Whiley DM, Buda PJ, Bayliss J, et al. A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene. Eur J Clin Microbiol Infect Dis 2004;23:705-710 (Pubitemid 39304326)
5. Whiley DM, Tapsall JW, Sloots TP. Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge. J Mol Diagn 2006;8:3-15.
6. Chaudhry U, Ray K, Bala M, et al. Multiplex polymerase chain reaction assay for the detection of Neisseria gonorrhoeae in urogenital specimens. Curr Sci 2002;83:634-640 (Pubitemid 135693196)
7. Farrell DJ. Evaluation of AMPLICOR Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR. J Clin Microbiol 1999;37:386-390
8. Palmer HM, Mallinson H, Wood RL, et al. Evaluation of the specificities of five DNA amplification methods for the detection of Neisseria gonorrhoeae. J Clin Microbiol 2003;41:835-837 (Pubitemid 36184209)
9. Martin IM, Foreman E, Hall V, et al. Non-cultural detection and molecular genotyping of Neisseria gonorrhoeae from a piece of clothing. J Med Microbiol 2007;56:487-490 (Pubitemid 46626565)
10. Unemo M, Palmer HM, Blackmore T, et al. Global transmission of prolyliminopeptidase-negative Neisseria gonorrhoeae strains: implications for changes in diagnostic strategies. Sex Transm Infect 2007;83:47-51. (Pubitemid 46293805)
11. Smith DW, Tapsall JW, Lum G. Guidelines for the use and interpretation of nucleic acid detection tests for Neisseria gonorrhoeae in Australia: a position paper on behalf of the Public Health Laboratory Network. Commun Dis Intell 2005;29:358-365
12. Bachmann LH, Johnson RE, Cheng H, et al. Nucleic acid amplification tests for diagnosis of Neisseria gonorrhoeae oropharyngeal infections. J Clin Microbiol 2009;47:902-907
13. Moncada J, Schachter J, Hook EW, et al. The effect of urine testing in evaluations of the sensitivity of the Gen-Probe Aptima Combo 2 assay on endocervical swab for Chlamydia trachomatis and Neisseria gonorrhoeae: the infected patient standard reduces sensitivity of single site evaluation. Sex Transm Dis 2004;31:273-277
14. Goire N, Nissen MD, Lecornec GM, et al. A duplex Neisseria gonorrhoeae real-time polymerase chain reaction assay targeting the gonococcal porA pseudogene and multicopy opa genes. Diagn Microbiol Infect Dis 2008;61:6-12. (Pubitemid 351509746)
15. Whiley DM, Anderson TP, Barratt K, et al. Evidence that the gonococcal porA pseudogene is presenting a broad range of Neisseria gonorrhoeae strains: suitability as a diagnostic target. Pathology 2006;38:445-448
16. Viscidi RP, Demma JC. Genetic diversity of Neisseria gonorrhoeae housekeeping genes. J Clin Microbiol 2003;41:197-204.