Implementation of adaptive optics in fluorescent microscopy using wavefront sensing and correction

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 7595, Issue , 2010, Pages

  Azucena, Oscar ^a   Crest, Justin ^b   Cao, Jian ^b   Sullivan, William ^b   Kner, Peter ^c   Gavel, Donald ^b   Dillon, Daren ^b   Olivier, Scot ^d   Kubby, Joel ^a  

^a University of California Santa Cruz   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

Author keywords

Adaptive optics; Biological imaging; Drosophila melanogaster; Fluorescent microscopy; Shack Hartmann wavefront sensor

Indexed keywords

BIOLOGICAL IMAGING; DROSOPHILA MELANOGASTER; FLUORESCENT MICROSCOPY; SHACK-HARTMANN WAVEFRONT SENSOR; SHACK-HARTMANN WAVEFRONT SENSORS;

ABERRATIONS; DEFORMATION; FLUORESCENCE; GEOMETRICAL OPTICS; MICROMETERS; MIRRORS; OPTOELECTRONIC DEVICES; REAL TIME SYSTEMS; SENSORS; TIME MEASUREMENT; WAVEFRONTS;

ADAPTIVE OPTICS;

EID: 77951658238     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.846380     Document Type: Conference Paper

References (19)

1. Van Helden, A., The Invention of the Telescope, Trans. Am. Phil. Soc. 67, pp. 20-21 (1977).
2. A. Dunn, Light scattering properties of cells, Dissertation, University of Texas, Austin http://www.nmr.mgh.harvard.edu/~adunn/papers/dissertation/ node7.html, 1998.
3. M. Schwertner, Specimen-induced distortions in light microscopy, J. Microscopy 228, pp. 97-102 (2007).
4. M. Schwertner, M. J. Booth, M. A.A. Neil & T. Wilson, Measurement of specimen-induced aberrations of biological samples using a phase stepping interferometer, 213, pp. 11-19 (2003).
5. Neil A. Campbell, Jane B. Reece , Biology, Benjamin Cummings, San Francisco. 2002.
6. Babcock, H. W., The possibility of compensating astronomical seeing, Pub. Astron. Soc. Pac., pp. 229-236 (1953).
7. Hardy, J. W., Adaptive Optics for Astronomical Telescopes, Oxford University Press, New York. 1998.
8. Liang J., B. Grim, S. Goelz, and J. F. Bille, Objective measurement of wave aberrations of the human eye with the use of a Hartmann-Shack wavefront sensor, J. Opt. Soc. of Am A 11, pp. 1949-1957, (1994).
9. J. Liang, D. R. Williams, D. T. Miller, Supernormal vision and high-resolution retinal imaging through adaptive optics, J. Opt. Soc. Am. A 14, pp. 2884-2892 (1997).
10. M. J. Booth, Adaptive optics in microscopy, Phil. Trans. A, Math Phys. Eng. Sci. 365, pp. 2829-2843 (2007).
11. Marcus Feierabend, Markus Ruckel, and Winfried Denk, Coherent-gated wavefront sensing in strongly scattering samples, Opt. Lett., 29, pp. 2255-2257 (2004).
12. L. D. S. Haro and J. C. Dainty, Single-pass measurements of the wave-front aberrations of the human eye by use of retinal lipofuscin autofluorescence, Opt. Lett. 24, pp. 61-63 (1999).
13. J. L. Beverage, R. V. Shack, and M. R. Descour, Measurement of the three-dimensional microscope point spread function using a Shack-Hartmann wavefront sensor, J. Microscopy 205, pp. 61-75 (2002).
14. Invitrogen Corporation, Fluorescence SpectraViewer, http://www. invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence- SpectraViewer.reg.us.html, Last Accessed: 8/15/2008.
15. Rothwell, W.F., and W. Sullivan, Fluorescent analysis of Drosophila embryos. In Drosophila Prot., W. Sullivan, M. Ashburner, and R.S. Hawley, editors. Cold Spring Harbor Lab. Press, Cold Spring Harbor, NY. pp. 141-157 (2000).
16. S. Thomas, T. Fusco, A. Tokovinin, M. Nicolle, V. Michau and G. Rousset, Comparison of centroid computation algorithms in a Shack-Hartmann sensor, MNRAS 371, pp. 323-336 (2006).
17. Lisa A. Poyneer, D. T. Gavel, J. M. Brase, Fast wave-front reconstruction in large adaptive optics systems with use of the Fourier transform, J. Opt. Soc. Am. A, 19, pp. 2100-2111 (2003).
18. J. Porter, H. Queener, J. Lin, K. Thorn, A. Awwal, Adaptive Optics for Vision Science, Wiley-Interscience, New Jersey, 2006.
19. Oscar Azucena, Joel Kubby, Justin Crest, Jian Cao, William Sullivan, Peter Kner, Donald Gavel, Daren Dillon, Scot Olivier, Implementation of a Shack-Hartmann wavefront sensor for the measurement of embryo-induced aberrations using fluorescent microscopy, Proc. SPIE 7209, p. 720906 (2009).