Isolation of cell wall mutants in Aspergillus nidulans by screening for hypersensitivity to Calcofluor White

Mycologia

Volumn 98, Issue 3, 2006, Pages 399-409

  Hill, Terry W ^a   Loprete, Darlene M ^a   Momany, Michelle ^b   Ha, Youngsil ^b   Harsch, Lisa M ^a   Livesay, Jennifer A ^a   Mirchandani, Amit ^a   Murdock, Jeremy J ^a   Vaughan, Michael J ^a   Watt, Mridula B ^a  

^a RHODES COLLEGE   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

Calcofluor hypersensitivity; Cell wall mutants; Swollen cell phenotype

Indexed keywords

GENETIC ISOLATION; MUTATION; SPORE; SWELLING; YEAST;

BACTERIA (MICROORGANISMS); EMERICELLA NIDULANS; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 77951621471     PISSN: 00275514     EISSN: 15572536     Source Type: Journal    
DOI: 10.3852/mycologia.98.3.399     Document Type: Article

References (54)

1. Aufauvre-Brown A, Mellado E, Gow NAR, Holden DW. 1997. Aspergillus fumigatus chsE: a gene related to CHS3 of Saccharomyces cerevisiae and important for hyphal growth and conidiophore development but not pathogenicity. Fungal Genet Biol 21:141-52.
2. Bainbridge BW, Valentine BP, Markham P. 1979. The use of temperature-sensitive mutants to study wall growth. In: Burnett JH, Trinci APJ, eds. Fungal walls and hyphal growth. London: Cambridge University Press. p 71-91.
3. 2+-type ATPase, is required for glycosylation and virulence. J Biol Chem 280:23408-23415.
4. Beauvais A, Latgé J-P. 2001. Membrane and cell wall targets in Aspergillus fumigatus. Drug Resist Updat 4:38-49.
5. Borkovich KA, Alex LA, Yarden O, Freitag M, Turner GE, Read ND, Seiler S, Bell-Pedersen D, Paietta J, Plesofsky N, Plamann M, Goodrich-Tanrikulu M, Schulte U, Mannhaupt G, Nargang FE, Radford A, Selitrennikoff C, Galagan JE, Dunlap JC, Loros JJ, Catcheside D, Inoue H, Aramayo R, Polymenis M, Selker EU, Sachs MS, Marzluf GA, Paulsen I, Davis R, Ebbole DJ, Zelter A, Kalkman ER, O'Rourke R, Bowring F, Yeadon J, Ishii C, Suzuki K, Sakai W, Pratt R. 2004. Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism. Microbiol Mol Biol Rev 68:1-108.
6. Bowman JC, Hicks PS, Kurtz MB, Rosen H, Schmatz DM, Liberator PA, Douglas CM. 2002. The antifungal echinocandin caspofungin acetate kills growing cells of Aspergillus fumigatus in vitro. Antimicrob Agents Chemother 46:3001-3012.
7. Carnero E, Ribas JC, Garcia B, Durán A, Sanchez Y. 2000. Schizosaccharomyces pombe ehs1p is involved in maintaining cell wall integrity and in calcium intake. Mol Gene Genet 264:173-183.
8. Clutterbuck AJ, Arst HN. 1995. Aspergillus nidulans. Trends Genet Supplement: Genetic nomenclature guide p 13-14.
9. d'Enfert C. 1997. Fungal spore germination: insights from the molecular genetics of Aspergillus nidulans and Neurospora crassa. Fungal Genet Biol 21:163-172.
10. Damveld RA, vanKuyk PA, Arentshorst M, Klis FM, van den Hondel CA, Ram AF. 2005. Expression of agsA, one of five 1,3-α-D-glucan synthase-encoding genes in Aspergillus niger, is induced in response to cell wall stress. Fungal Genet Biol 42:165-177.
11. de Groot PWJ, Ram AF, Klis FM. 2005. Features and functions of covalently linked proteins in fungal cell walls. Fungal Genet Biol 42:657-75.
12. _, Ruiz C, Vázquez de Aldana CR, Dueňas E, Cid VJ, Del Rey F, Rodríguiz-Peña JM, Pérez P, Andel A, Caubín J, Arroyo J, García JC, Gil C, Molina M, García LJ, Nombela C, Klis FM. 2001. A genomic approach for the identification, classification of genes involved in cell wall formation and its regulation in Saccharomyces cerevisiae. Comp Funct Genomics 2:124-142.
13. Durán A, Nombela C. 2004. Fungal cell wall biogenesis: building a dynamic interface with the environment. Microbiology 150:3099-3103.
14. Elorza MV, Rico H, Sentandreu R. 1983. Calcofluor White alters the assembly of chitin fibrils in Saccharomyces cerevisiae and Candida albicans cells. J Gen Microbiol 129:1577-1582.
15. Fujiwara M, Ichinomiya M, Motoyama T, Horiuchi H, Ohta A, Takagi M. 2000. Evidence that the Aspergillus nidulans class I and class II chitin synthase genes, chsC, chsA, share critical roles in hyphal wall integrity and conidiophore development. J Biochem 127:359-366.
16. Guest GM, Lin X, Momany M. 2004. Aspergillus nidulans RhoA is involved in polar growth, branching, and cell wall synthesis. Fungal Genet Biol 41:13-22.
17. _, Momany M. 2000. Analysis of cell wall sugars in the pathogen Aspergillus fumigatus and the saprophyte Aspergillus nidulans. Mycologia 92:1047-1050.
18. Harris SD, Morrell JL, Hamer JE. 1994. Identification and characterization of Aspergillus nidulans mutants defective in cytokinesis. Genetics 136:517-532.
19. Hill TW, Kafer E. 2001. Improved protocols for Aspergillus minimal medium: trace element and minimal medium salt stock solutions. Fungal Genet Newsletter 48:20-21.
20. Hoffmann JA, Kafatos FC, Janeway CA, Ezekowitz RAB. 1999. Phylogenetic perspectives in innate immunity. Science 284:1313-1318.
21. Horiuchi H, Fujiwara M, Yamashita S, Ohta A, Takagi M. 1999. Proliferation of intrahyphal hyphae caused by disruption of csmA, which encodes a class V chitin synthase with a myosin motor-like domain in Aspergillus nidulans. J Bacteriol 181:3721-3729.
22. Imazu H, Sakurai H. 2005. Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock. Eukaryot Cell 4:1050-1056.
23. Kafer E. 1977. Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet 19:33-131.
24. Kaminskyj SGW. 2001. Fundamentals of growth, storage, genetics and microscopy of Aspergillus nidulans. Fungal Genet Newsletter 48:25-31.
25. Kapteyn JC, Van Den Ende H, Klis FM. 1999. The contribution of cell wall proteins to the organization of the yeast cell wall. Biochim Biophys Acta 1426:373-83.
26. King J, Haase-Pettingell C, Gossard D. 2002. Protein folding and misfolding. Am Sci 90:445-453.
27. Levin DE. 2005. Cell wall integrity signaling in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 69:262-291.
28. Lussier M, White A-M, Sheraton J, di Paolo T, Treadwell J, Southard SB, Horenstein CI, Chen-Weiner J, Ram AFJ, Kapteyn JC, Roemer TW, Vo DH, Bondoc DC, Hall J, Zhong WW, Sdicu A-M, Davies J, Klis FM, Robbins PW, Bussey H. 1997. Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147:435-450.
29. Maeda H, Ishida N. 1967. Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener. J Biochem 62:276-8.
30. Markovich S, Yekutiel A, Shalit I, Shadkchan Y, Osherov N. 2004. Genomic approach to identification of mutations affecting caspofungin susceptibility in Saccharomyces cerevisiae. Antimicrob Agents Chemother 48:3871-3876.
31. Martin CE, DeBusk AG. 1975. Temperature-sensitive, osmotic-remedial mutants of Neurospora crassa: osmotic-pressure-induced alterations of enzyme stability. Mol Gen Genet 136:31-40.
32. Martín V, García B, Carnero E, Durán A, Sánchez Y. 2003. Bgs3p, a putative 1,3-b-glucan synthase subunit, is required for cell wall assembly in Schizosaccharomyces pombe. Eukaryot Cell 2:159-169.
33. Matsuoka H, Yang H-C, Homma T, Nemoto Y, Yamada S, Sumita O, Takatori K, Kurata H. 1995. Use of Congo red as a microscopic fluorescence indicator of hyphal growth. Appl Microbiol Biotechnol 43:102-108.
34. Momany M. 2005. Growth control and polarization. Med Mycol Supplement 1 43:23-25.
35. _, Westfall PJ, Abramowsky G. 1999. Aspergillus nidulans swo mutants show defects in polarity establishment, polarity maintenance and hyphal morphogenesis. Genetics 151:557-567.
36. Mulder HJ, Saloheimo M, Penttilä M, Madrid SM. 2004. The transcription factor HACA mediates the unfolded protein response in Aspergillus niger and up-regulates its own transcription. Mol Genet Genomics 271:130-140.
37. Müller C, Hjort CM, Hansen K, Nielsen J. 2002. Altering the expression of two chitin synthase genes differentially affects the growth and morphology of Aspergillus oryzae. Microbiology 148:4025-4033.
38. Murgui A, Elorza MV, Sentandreu R. 1985. Effect of papulacandin B, Calcofluor White on the incorporation of mannoproteins in the wall of Candida albicans blastospores. Biochim Biophys Acta 841:215-222.
39. Oka T, Hamaguchi T, Sameshima Y, Goto M, Furukawa K. 2004. Molecular characterization of protein O-mannosyltransferase and its involvement in cell-wall synthesis in Aspergillus nidulans. Microbiology 150:1973-1982.
40. Osherov N, Mathew J, May GS. 2000. Polarity-defective mutants of Aspergillus nidulans. Fungal Genet Biol 31:181-188.
41. Pancaldi S, Poli F, Dall'Olio G, Vannini GL. 1984. Morphological anomalies induced by Congo red in Aspergillus niger. Arch Microbiol 137:185-187.
42. Ram AFJ, Wolters A, Ten Hoopen R, Klis FM. 1994. A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to Calcofluor White. Yeast 10:1019-1030.
43. Raper KB, Fennell DI. 1965. The genus Aspergillus. Baltimore: Williams & Wilkins. 686 p.
44. Rementeria A, López-Molina N, Ludwig A, Vivanco AB, Bikandi J, Pontón J, Garaizar J. 2005. Genes and molecules involved in Aspergillus fumigatus virulence. Rev Iberoam Micol 22:1-23.
45. Roncero C, Durán A. 1985. Effect of Calcofluor White and Congo red on fungal cell wall morphogenesis: in vivo activation of chitin polymerization. J Bacteriol 163:1180-1185.
46. Selitrennikoff CP. 2001. Antifungal Proteins. Appl Environ Microbiol 67:2883-2894.
47. Shaw BD, Momany M. 2002. Aspergillus nidulans polarity mutant swoA is complemented by protein O-mannosyltransferase pmtA. Fungal Genet Biol 37:263-270.
48. _, Upadhyay S. 2005. Aspergillus nidulans swoK encodes an RNA binding protein that is important for cell polarity. Fungal Genet Biol 42:862-872.
49. Tkacz JS, Lampen O. 1975. Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes. Biochem Biophys Res Commun 65:248-257.
50. Wendland J. 2001. Comparison of morphogenetic networks of filamentous fungi and yeast. Fungal Genet Biol 34:63-82.
51. Wood PJ. 1980. Specificity in the interaction of direct dyes with polysaccharides. Carbohydr Res 85:271-287.
52. Yamada E, Ichinomiya M, Ohta A, Horiuchi H. 2005. The class V chitin synthase gene csmA is crucial for growth of the chsA chsC double mutant in Aspergillus nidulans. Biosci Biotechnol Biochem 69:87-97.
53. Yanai K, Kojima N, Takaya N, Horiuchi H, Ohta A, Takagi M. 1994. Isolation and characterization of two chitin synthase genes from Aspergillus nidulans. Biosci Biotechnol Biochem 58:1828-1835.
54. York WS, Darvill AG, McNeil M, Stevenson TT, Albersheim P. 1986. Isolation and characterization of cell walls and cell wall components. Methods Enzymol 118:3-40.