Vertical sandwich-type continuous/evaporative TLC with fixed mobile phase volume for separating sugars of clinical relevance in paper-borne urine and blood samples in newborn screening

Journal of Clinical Laboratory Analysis

Volumn 24, Issue 2, 2010, Pages 106-112

  Alonso Fernandez, J R ^a   Carpinteiro, M I ^a   Baleato, J ^a   Fidalgo, J ^a  

^a HOSPITAL CLÍNICO UNIVERSITARIO   (Spain)

Author keywords

Continuous TLC; Dried urine on paper; Galactosaemia; Glycosurias; Neonatal diabetes; Newborn screening; Oligosaccharides; Saccharides; Sandwich TLC; Sugars

Indexed keywords

EPIMERASE; GALACTO 4 EPIMERASE; GALACTOKINASE; UNCLASSIFIED DRUG;

ARTICLE; BLOOD SAMPLING; DIABETES MELLITUS; ENZYME DEFICIENCY; FALSE NEGATIVE RESULT; GALACTO 4 EPIMERASE DEFICIENCY; GALACTOKINASE DEFICIENCY; GALACTOSEMIA; GLUCOSURIA; HUMAN; NEWBORN; NEWBORN SCREENING; THIN LAYER CHROMATOGRAPHY; URINALYSIS;

CARBOHYDRATES; CHROMATOGRAPHY, THIN LAYER; DIABETES MELLITUS; GALACTOSE; GALACTOSEMIAS; HUMANS; INFANT, NEWBORN; INFANT, NEWBORN, DISEASES; NEONATAL SCREENING; PAPER;

EID: 77951600795     PISSN: 08878013     EISSN: 10982825     Source Type: Journal    
DOI: 10.1002/jcla.20371     Document Type: Article

References (26)

1. Berry HK, Sutherland B, Guest GM, Warkainy J. Simple method for detection of phenylketonuria. JAMA 1958;167:2189-2190.
2. Berry HK. Procedures for testing urine specimens dried on filter Paper. Clin Chem 1959;5:603-608.
3. Das AK, Sakker IB. Action of amines on quinolinic acid. J Ind Chem Soc 1934;11:707-710.
4. Woolf LI. Large scale screening for metabolic disease in the newborn in Great Britain. In: JA Anderson, KF Swaiman, editors. Phenylketonuria and Allied Metabolic Diseases. Washington: Children's Bureau. 1967. p 50-61.
5. Alonso-Fernández JR, Castiñeiras DE, Parrado C, Fraga JM, Peña J. Galactose newborn screening: test for reducing sugars in urine samples impregnated on paper. In: BL Jr Therrell, editor. Advances in Neonatal Screening. Amsterdam: Elsevier Science Publishers B.V. (Biomedical Division). 1987. p 233-238.
6. Giménez-Serrat M, Julve Pérez E. Protocolo de dos métodos de detección cualitativa de sustancias reductoras en orina impregnada en papel. Dispensario Médico, Diagnóstico y Prevención, Caja de Ahorros y Monte de Piedad de Zaragoza, Aragón y la Rioja. Personal communication, June 15th 1993.
7. Harper HA. Review of Physiologic Chemistry, Twelfth edition. Los Altos, CA: Lange Medical Publications. 1969. p 11.
8. In Shih VE. Anthrone Test for Carbohydrates in Laboratory Techniques for Detection of Hereditary Metabolic Disorders. Boston: CRC Press. 1973. p 78.
9. Auray-Blais C, Giguere R, Draper P, Shapcott D, Lenieux B. Simple and rapid system for screening and identification of reducing sugars in urine. Clin Biochem 1978;11:235-237.
10. Ng WG, Kamura M, Donnel GM. Galactosemia screening: Methodology and outcome from Worldwide data collection. In: BL Jr Therrell, editor. Advances in Neonatal Screening. Amsterdam: Elsevier Science Publishers B.V. (Biomedical Division). 1987. p 243-249.
11. Webster D, Allen DN. Laboratory methods for galactosemia testing in neonatal screening. In: BL Therrell Jr, editor. Laboratory Methods for Neonatal Screening. Washington: American Public Health Association. 1993. p 77-114.
12. Kaye CI, Committee on Genetics. Newborn screening fact sheets. Pediatrics 2006;118:934-963.
13. Alonso-Fernández JR. Tría Neonatal de Galactosemia: Situación del Ensayo en Orina. Rev Lab Clin 2008;1:133-134
14. Haldorsen KM. Quantitative thin-layer chromatography of carbohydrates using pentavalent vanadium in sulfuric acid. J Chromatogr 1978;150:485-490.
15. Geiss F. Fundamentals of Thin-layer Chromatography (Planar Chromatography). Basel and New York: Dr. Alfred Hüting Verlag, Heildelberg. 1987. p 129-144.
16. Alonso-Fernández JR, Quintas R, Peña J, Fraga JM. A method for the screening, separation, identification and semiquantitative assessment of sugars of metabolic interest in urine samples impregnated on paper. International Symposium on Inborn Errors of Metabolism in Humans. Interlaken (Switzerland). September 1980.
17. Alonso-Fernández JR, Bóveda MD, Parrado C, Peña J, Fraga JM. Continuous thin-layer chromatography of sugars of clinical interest in samples of urine impregnated on paper. J Chromatogr 1981;217:357-366.
18. Alonso-Fernández JR, Parrado C, Bóveda MD, Peña J, Fraga JM. Inborn errors of metabolism of carbohydrates: A new method for the detection and identification of sugars in urine and blood from samples impregnated on paper. In: H Naruse, W Irie, editors. Neonatal Screening. Amsterdam: Excerpta Medica. 1982. p 256-257.
19. Alonso-Fernández JR, Castiñeiras C, Villar P, Cocho JA, Bóveda MD. Continuous-evaporative chromatography with a discrete volumen of elution in the detection and classification of galactosemias. In: HL Levy, RJ Hermos, GF Grady, editors. Proceedings of the Third Meeting of the International Society for Neonatal Screening, Jamaica Plain, MA, USA. 1996. p 129-130.
20. Alonso-Fernández JR, Castiñeiras C, Cocho JA, Bóveda MD, Castiñeiras DE. Tría neonatal de galactosemias empleando la muestra de orina. III Congreso Nacional, Errores Innatos del Metabolismo. Santiago de Compostela (Spain), November 25-27, 1999. In Errores Innatos del Metabolismo52000, Edita: Servicio de Publicaciones e Intercambio Cientifico, Universidad de Santiago de Compostela. ISBN: 84-8121-794-8. 1999. p 149-150.
21. Alonso-Fernández JR, Carpintero MI, Baleato J, Fidalgo J. Continuous Evaporative TLC with Small Eluent Volumes for Saccharides and Oligosaccharides of Clinical Interest. VI Reunión Científica de la SECyTA, Vigo, Spain, November 8-10, 2006.
22. Menzies IS, Mount JN. Advantages of silica gel as a médium for rapid thin layer chromatography of neutral sugars. Med Lab Tech 1975;32:269-276.
23. Regis Technical Manual. Regis Chemical Co., 8210 Austin Avenue, Norton Grove, IL 60053. 1979.
24. Müthing J. High-resolution thin-layer chromatography of gangliosides. J Chromatogr A 1996;720:3-25.
25. Jensen NG, Brandt NJ, Christensen E, Skovby F, Nørgaard-Pedersen B, Simonsen H. Neonatal screening for galactosemias by quantitative analysis of hexose monophosphates using tandem mass spectrometry. A retrospective study. Clin Chem 2001;47: 1364-1372.
26. Erser RS, Smiths I. Aminoacids and related compounds. In Smith I, Seakins ., editors. Chromatography and Electrophoretic Techniques, Fourth edition. William Heinemann Medical Books Ltd. 1976, p 79.