Functional comparison of hemoglobin purified by different methods and their biophysical implications

Biotechnology and Bioengineering

Volumn 106, Issue 1, 2010, Pages 76-85

  Elmer, Jacob ^a   Buehler, Paul W ^b   Jia, Yiping ^b   Wood, Francine ^b   Harris, David R ^a   Alayash, Abdu I ^b   Palmer, Andre F ^a  

^a Ohio State University   (United States)

^b CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

Anion exchange chromatography; Blood substitute; Hemoglobin; Hemoglobin based oxygen carriers; Purification; Red blood cell; Tangential flow filtration; Transfusion

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; BIOPHYSICAL PROPERTIES; CAMBRIDGE; CHROMATOGRAPHIC SEPARATIONS; COLUMN SEPARATION; HEMOGLOBIN-BASED OXYGEN CARRIERS; HIGH TEMPERATURE; LIGAND BINDING; MASS CHANGE; PHYSIOLOGICAL CONDITION; PROCESSING CONDITION; PROSTHETIC GROUPS; PROTEIN STRUCTURES; RED BLOOD CELL; SDS-PAGE; STRUCTURAL ALTERATIONS; TANGENTIAL FLOW; TORONTO , CANADA;

BLOOD; BLOOD SUBSTITUTES; CELLS; HEAT TREATING FURNACES; HEMOGLOBIN; HEMOGLOBIN OXYGEN SATURATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; OXIDATION; OXYGEN; PHYSIOLOGICAL MODELS; PORPHYRINS; STEADY FLOW;

PURIFICATION;

CARBON MONOXIDE; CATALASE; HEMOGLOBIN; NITRIC OXIDE; OXYGEN; SODIUM CHLORIDE;

ARTICLE; AUTOOXIDATION; BINDING KINETICS; CONTROLLED STUDY; DISSOCIATION; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ERYTHROCYTE; FILTRATION; GEL PERMEATION CHROMATOGRAPHY; LIGAND BINDING; MOLECULAR WEIGHT; OXYGEN AFFINITY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; TEMPERATURE SENSITIVITY;

ANIMALS; CANADA; CATTLE; ERYTHROCYTES; FILTRATION; HEMOGLOBINS; HUMANS;

BOVINAE;

EID: 77951547437     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22659     Document Type: Article

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