Effect of neutrase, alcalase, and papain hydrolysis of whey protein concentrates on iron uptake by Caco-2 cells

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 8, 2010, Pages 4894-4900

  Ou, Keqin ^a   Liu, Yunzhu ^a   Zhang, Liebing ^a   Yang, Xiaoguang ^b   Huang, Zhenwu ^b   Nout, M J Robert ^c   Liang, Jianfen ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b BEIJING HOSPITAL   (China)

^c WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Caco 2 cells; Enzymatic hydrolysates; In vitro digestion; Iron absorption

Indexed keywords

IRON; METALLOPROTEINASE; MICROBIAL METALLOPROTEINASES; PAPAIN; SUBTILISIN;

ARTICLE; CELL STRAIN CACO 2; HUMAN; HYDROLYSIS; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS;

CACO-2 CELLS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROLYSIS; IRON; METALLOENDOPEPTIDASES; PAPAIN; SUBTILISINS;

EID: 77951283238     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf100055y     Document Type: Article

References (31)

1. Liang, J.; Han, B.; Nout, M. J. R.; Hamer, R. J. Effect of soaking and phytase treatment on phytic acid, calcium, iron and zinc in rice fractions Food Chem. 2009, 115, 789-794
2. Liang, J.; Han, B.; Nout, M. J. R.; Hamer, R. J. Effects of soaking, germination and fermentation on phytic acid, total and in vitro soluble zinc in brown rice Food Chem. 2008, 110, 821-828
3. Yu, B. Attention on iron fortification-the application of NaFeEDTA in food. Sci. Technol. Food Ind. 2007, 4) 34-35 (in Chinese).
4. Ishikawa, S. I.; Tamaki, S.; Arihara, K.; Itoh, M. Egg yolk protein and egg yolk phosvitin inhibit calcium, magnesium, and iron absorptions in rats J. Food Sci. 2007, 72, S412-S419
5. Etcheverry, P.; Miller, D. D.; Glahn, R. P. A low-molecular-weight factor in human milk whey promotes iron uptake by Caco-2 cells J. Nutr. 2004, 134, 93-98
6. Lynch, S. R. Interaction of iron with other nutrients Nutr. Rev. 1997, 55, 102-110
7. De Souza Nogueira, C.; Colli, C.; Maria Silverio Amancio, O. Infant formula iron dialysability related to other nutrients Food Chem. 2005, 90, 779-783
8. Sugiarto, M.; Ye, A.; Singh, H. Characterisation of binding of iron to sodium caseinate and whey protein isolate Food Chem. 2009, 114, 1007-1013
9. Remondetto, G. E.; Beyssac, E.; Subirade, M. Iron availability from whey protein hydrogels: an in vitro study J. Agric. Food Chem. 2004, 52, 8137-8143
10. Storcksdieck genannt Bonsmann, S.; Hurrell, R. F. Iron-binding properties, amino acid composition, and structure of muscle tissue peptides from in vitro digestion of different meat sources J. Food Sci. 2007, 72, S019-S029
11. Kim, M.; Lee, D. T.; Lee, Y. S. Iron absorption and intestinal solubility in rats are influenced by dietary proteins Nutr. Res. (N.Y.) 1995, 15, 1705-1716
12. Mulvihill, B.; Morrissey, P. A. Influence of the sulphydryl content of animal proteins on in vitro bioavailability of non-haem iron Food Chem. 1998, 61, 1-7 (Pubitemid 28152149)
13. Kim, S. B.; Seo, I. S.; Khan, M. A.; Ki, K. S.; Nam, M. S.; Kim, H. S. Separation of iron-binding protein from whey through enzymatic hydrolysis Int. Dairy J. 2007, 17, 625-631
14. Lee, S. H.; Song, K. B. Purification of an iron-binding nona-peptide from hydrolysates of porcine blood plasma protein Process Biochem. 2009, 44, 378-381
15. Kim, S. B.; Seo, I. S.; Khan, M. A.; Ki, K. S.; Lee, W. S.; Lee, H. J.; Shin, H. S.; Kim, H. S. Enzymatic hydrolysis of heated whey: Iron-binding ability of peptides and antigenic protein fractions J. Dairy Sci. 2007, 90, 4033-4042
16. Smithers, G. W. Whey and whey proteins-from 'gutter-to-gold' Int. Dairy J. 2008, 18, 695-704
17. Adler-Nissen, J. Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid J. Agric. Food Chem. 1979, 27, 1256-1262
18. Kiers, J. L.; Van Laeken, A.; Rombouts, F. M.; Nout, M. In vitro digestibility of Bacillus fermented soya bean Int. J. Food Microbiol. 2000, 60, 163-169
19. Glahn, R. P.; Lee, O. A.; Yeung, A.; Goldman, M. I.; Miller, D. D. Caco-2 cell ferritin formation predicts nonradiolabeled food iron availability in an in vitro digestion/Caco-2 cell culture model J. Nutr. 1998, 128, 1555-1561
20. Glahn, R. P.; Gangloff, M. B.; van Campen, D. R.; Miller, D. D.; Wien, E. M.; Norvell, W. A. Bathophenanthrolene disulfonic acid and sodium dithionite effectively remove surface-bound iron from Caco-2 cell monolayers J. Nutr. 1995, 125, 1833-1840
21. Gargari, B. P.; Razavieh, S. V.; Mahboob, S.; Niknafs, B.; Kooshavar, H. Effect of retinol on iron bioavailability from Iranian bread in a Caco-2 cell culture model Nutrition 2006, 22, 638-644
22. Schagger, H.; Von Jagow, G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa Anal. Biochem. 1987, 166, 368-379
23. Kim, M.; Lee, D. T.; Lee, Y. S. Iron absorption and intestinal solubility in rats are influenced by dietary proteins Nutr. Res. (N.Y.) 1995, 15, 1705-1716
24. Hurrell, R. F.; Lynch, S. R.; Trinidad, T. P.; Dassenko, S. A.; Cook, J. D. Iron absorption in humans as influenced by bovine milk proteins Am. J. Clin. Nutr. 1989, 49, 546-552
25. Perales, S.; Barber, R.; Lagarda, M. J.; Farr, R. Availability of iron from milk-based formulas and fruit juices containing milk and cereals estimated by in vitro methods (solubility, dialysability) and uptake and transport by Caco-2 cells Food Chem. 2007, 102, 1296-1303
26. Kim, S. B.; Seo, I. S.; Khan, M. A.; Ki, K. S.; Nam, M. S.; Kim, H. S. Separation of iron-binding protein from whey through enzymatic hydrolysis Int. Dairy J. 2007, 17, 625-631
27. Sukan, G.; Andrews, A. T. Application to the plastein reaction to casein and skim-milk powder J. Dairy Res 1982, 49, 265-78
28. Chaud, M. V.; Izumi, C.; Nahaal, Z.; Shuhama, T.; Bianchi, M.; Freitas, O. Iron derivatives from casein hydrolysates as a potential source in the treatment of iron deficiency J. Agric. Food Chem. 2002, 50, 871-877
29. Argyri, K.; Miller, D. D.; Glahn, R. P.; Zhu, L.; Kapsokefalou, M. Peptides isolated from in vitro digests of milk enhance iron uptake by Caco-2 cells J. Agric. Food Chem. 2007, 55, 10221-10225
30. 26Ala Protein Sci. 1998, 7, 1875-1883
31. Yaron, A.; Naider, F.; Scharpe, S. Proline-dependent structural and biological properties of peptides and proteins Crit. Rev. Biochem. Mol. Biol. 1993, 28, 31-81