Two-dimensional NMR and all-atom molecular dynamics of cytochrome P450 CYP119 reveal hidden conformational substates

Journal of Biological Chemistry

Volumn 285, Issue 13, 2010, Pages 9594-9603

  Lampe, Jed N ^a   Brandman, Relly ^b   Sivaramakrishnan, Santhosh ^a   Ortiz De Montellano, Paul R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Simprota Corporation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; AFFINITY LIGANDS; ANTIBIOTIC SYNTHESIS; BIOLOGICAL PROCESS; CONFORMATIONAL SELECTION; CONFORMATIONAL SUBSTATES; CYTOCHROME P450; HETERONUCLEAR SINGLE-QUANTUM COHERENCES; HORMONAL REGULATION; HYDROPHOBIC LIGAND; INDUCED FIT; LIGAND BINDING; LIGAND-FREE; MOLECULAR DYNAMICS SIMULATIONS; NMR SPECTROSCOPY; SITE-SPECIFIC; TIGHT BINDING; WEAK BINDING;

AMINO ACIDS; CATALYSTS; ENZYMES; MOLECULAR DYNAMICS; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; ORGANIC ACIDS; QUANTUM THEORY; SUBSTRATES; TWO DIMENSIONAL;

LIGANDS;

4 METHOXYPHENYLALANINE C 13; AMINO ACID DERIVATIVE; CYTOCHROME P450; CYTOCHROME P450 119; UNCLASSIFIED DRUG; ARCHAEAL PROTEIN; CYP119 PROTEIN, SULFOLOBUS SOLFATARICUS; LAURIC ACID; LAURIC ACID DERIVATIVE; LIGAND; METIROSINE; O-METHYLTYROSINE; ORTHO METHYLTYROSINE;

ARTICLE; BINDING AFFINITY; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SUBSTRATE; HETERONUCLEAR SINGLE QUANTUM COHERENCE; HYDROPHOBICITY; LIGAND BINDING; MOLECULAR DYNAMICS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; SIMULATION; SULFOLOBUS ACIDOCALDARIUS; BINDING SITE; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; METHODOLOGY; MUTATION; PROTEIN CONFORMATION; SPECTROPHOTOMETRY; X RAY CRYSTALLOGRAPHY;

SULFOLOBUS ACIDOCALDARIUS;

ARCHAEAL PROTEINS; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME P-450 ENZYME SYSTEM; LAURIC ACIDS; LIGANDS; MAGNETIC RESONANCE SPECTROSCOPY; METHYLTYROSINES; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; SPECTROPHOTOMETRY; SULFOLOBUS ACIDOCALDARIUS;

EID: 77951240144     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.087593     Document Type: Article

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