A hydrophilic interaction ultraperformance liquid chromatography (HILIC-UPLC) method for genomic DNA methylation assessment by UV detection

Analytical and Bioanalytical Chemistry

Volumn 396, Issue 8, 2010, Pages 2937-2941

  Sotgia, Salvatore ^a   Zinellu, Angelo ^a,b,c   Pisanu, Elisabetta ^a,b   Murgia, Luciano ^a   Pinna, Gerard Aime ^a   Gaspa, Leonardo ^a   Deiana, Luca ^a   Carru, Ciriaco ^a,b  

^a UNIVERSITY OF SASSARI   (Italy)

^b NATIONAL INSTITUTE OF BIOSTRUCTURES AND BIOSYSTEMS   (Italy)

^c Porto Conte Ricerche Srl   (Italy)

Author keywords

1.7 m ethylene bridged hybrid (BEH) particle; DNA methylation; Hydrophilic interaction chromatography (HILIC); Ultraperformance liquid chromatography (UPLC)

Indexed keywords

AMMONIUM ACETATE; DETERMINATION COEFFICIENTS; DNA METHYLATION; ETHYLENE-BRIDGED; GENOMIC DNA; HYBRID PARTICLES; HYDROPHILIC INTERACTION; HYDROPHILIC INTERACTION CHROMATOGRAPHY; ISOCRATIC ELUTION; LIMIT OF DETECTION; LIMIT OF QUANTIFICATIONS; METHYLCYTOSINE; MOBILE PHASE; PACKED COLUMN; ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY; UV DETECTION;

ALKYLATION; AMMONIUM COMPOUNDS; COLUMN CHROMATOGRAPHY; DNA; ETHYLENE; GENES; HYDROPHILICITY; LIQUIDS; METHYLATION;

LIQUID CHROMATOGRAPHY;

CALF THYMUS DNA; DNA; WATER;

ANIMAL; ARTICLE; CHEMISTRY; DNA METHYLATION; HUMAN; HUMAN GENOME; LIQUID CHROMATOGRAPHY; METHODOLOGY; ULTRAVIOLET SPECTROPHOTOMETRY;

ANIMALS; CHROMATOGRAPHY, LIQUID; DNA; DNA METHYLATION; GENOME, HUMAN; HUMANS; SPECTROPHOTOMETRY, ULTRAVIOLET; WATER;

EID: 77951233523     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-010-3565-3     Document Type: Article

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